FlyBase Allele Report: Dmel\Oli[Δ9]

General Information

Symbol

Dmel\Oli^Δ9

Species

D. melanogaster

Name

FlyBase ID

FBal0284100

Feature type

allele

Associated gene

Dmel\Oli

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Oyallon et al., 2012)

Progenitor genotype

P{GSV2}Oli^GS5080

(Oyallon et al., 2012)

Cytology

Description

Imprecise excision of the progenitor insertion resulting in a 1.53kb deletion extending from 802bp upstream to 732bp downstream of the Oli translation start site.

(Oyallon et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:17,589,438..17,590,972 [+]

Comment:

Reported as a 1.53 kb deletion resulting from the imprecise excision of P{GSV2}Oli^GS5080, which extends from 802 bp upstream to 732 bp downstream of the Oli translation start.

(Oyallon et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal intersegmental bidendritic neuron isbp | embryonic stage

(Oyallon et al., 2012)

larval intersegmental nerve branch ISNb of A1-7 | embryonic stage

(Oyallon et al., 2012)

larval transverse nerve | embryonic stage

(Oyallon et al., 2012)

Detailed Description

Statement

Reference

Approximately 80.4% of homozygous embryos survive to the larval stage. Under non-crowded conditions, only 42% of adults emerge from the pupal case. Under normal population conditions, only 3.4% of homozygotes emerge as adults.

Homozygous wandering stage third instar larvae remain largely on the surface on agar plates, in contrast to wild-type larvae which burrow into the agar at this stage. The mutant larvae show irregular, slower crawling behaviour compared to wild type and frequently appear to drag their abdominal segments. Homozygous adult escapers are unable to fly and have slow, uncoordinated leg movements, circling and wobbling.

The distribution and number of ventral nerve cord glia and the organisation of the ventral nerve cord scaffold appear normal in mutant embryos. The number and positions of eve-positive neurons also appear normal.

Btau\MAPT^{isl.H.T:Hsap\MYC}-positive motorneuron axons show abnormal trajectories at the ventral nerve cord exit in mutant embryos; they fail to exit via the transverse nerve (TN) in 54.6% of hemisegments and ectopically extend axons through the SN branch in 58.8% of hemisegments. ISNb and TN motorneurons show muscle targeting defects in 51.7% of hemisegments. In 19.3% of hemisegments, ISNb motorneuron axons do not correctly defasciculate from each other, resulting in the stalling of thicker axon bundles between muscles 6 and 13 and a failure to innervate the muscle 12/13 cleft. In 27.8% of hemisegments, ISNb or TN axons extend processes that form abnormal contacts between them and in 14.2% of hemisegments, lateral bipolar dendrite /TN neurons have ectopic processes onto ventral muscles.

(Oyallon et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Oli^Δ9 has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by exex^Gal4/exex^KK30

(Oyallon et al., 2012)

Suppressed by

Statement

Reference

Oli^Δ9 has lethal phenotype, suppressible | partially by Scer\GAL4^elav-C155/Ggal\Olig2^UAS.cOa

(Oyallon et al., 2012)

Oli^Δ9 has abnormal locomotor behavior | adult stage phenotype, suppressible | partially by Scer\GAL4^elav-C155/Ggal\Olig2^UAS.cOa

(Oyallon et al., 2012)

Enhancer of

Statement

Reference

Oli^Δ9/Oli^Δ9 is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of exex^Gal4/exex^KK30

(Oyallon et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

Oli^Δ9 has larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype, enhanceable by exex^Gal4/exex^KK30

(Oyallon et al., 2012)

Enhancer of

Statement

Reference

Oli^Δ9/Oli^Δ9 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of exex^Gal4/exex^KK30

(Oyallon et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of the phenotype seen in both Oli^Δ9 (19.3%) and exex^KK30/exex^Gal4 (34.4%) single mutant embryos where ISNb motorneuron axons do not correctly defasciculate from each other and fail to innervate the muscle 12/13 cleft is increased in double mutants to 68.5%.

(Oyallon et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of Ggal\Olig2^Scer\UAS.cOa under the control of Scer\GAL4^elav-C155 partially rescues eclosion rates and walking defects in Oli^Δ9 adults.

(Oyallon et al., 2012)

Complementation and Rescue Data

Partially rescued by

Oli^Δ9 is partially rescued by Scer\GAL4^elav-C155/Oli^UAS.cOa

(Oyallon et al., 2012)

Oli^Δ9 is partially rescued by Scer\GAL4^sca.PU/Oli^UAS.cOa

(Oyallon et al., 2012)

Oli^Δ9 is partially rescued by Scer\GAL4^VGlut-OK371/Oli^UAS.cOa

(Oyallon et al., 2012)

Not rescued by

Oli^Δ9 is not rescued by Oli^UAS.cOa/Scer\GAL4^repo

(Oyallon et al., 2012)

Comments

Expression of Oli^Scer\UAS.cOa under the control of Scer\GAL4^elav-C155 fails to rescue the motorneuron axon targeting defects seen in Oli^Δ9 embryos. Survival to adulthood and adult locomotor defects are significantly rescued.

Expression of Oli^Scer\UAS.cOa under the control of Scer\GAL4^sca.PU partially rescues the motorneuron axon targeting defects seen in Oli^Δ9 embryos. Survival to adulthood and adult locomotor defects are significantly rescued.

Expression of Oli^Scer\UAS.cOa under the control of Scer\GAL4^repo fails to rescue eclosion rates or walking defects in Oli^Δ9 adults.

Expression of Oli^Scer\UAS.cOa under the control of Scer\GAL4^VGlut-OK371 partially rescues eclosion rates and walking defects in Oli^Δ9 adults.

(Oyallon et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Oli^Δ9

(Guan et al., 2022)

oli^Δ9

(Oyallon et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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