FlyBase Allele Report: Dmel\lola[ORE120]

General Information

Symbol

Dmel\lola^ORE120

Species

D. melanogaster

Name

FlyBase ID

FBal0137145

Feature type

allele

Associated gene

Dmel\lola

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Crowner et al., 2002)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T9922C. Amino acid replacement: A107V.

(Horiuchi et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:10,533,366..10,533,366 [-]

Nucleotide change:

C10533366T

Reported nucleotide change:

C9922T

Amino acid change:

Reported amino acid change:

A107V

(Horiuchi et al., 2003, Crowner et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Horiuchi et al., 2003, Crowner et al., 2002)

lethal

(Crowner et al., 2002)

lethal (with Df(2R)stan2)

(Horiuchi et al., 2003)

lethal (with lola^c46)

(Horiuchi et al., 2003)

lethal | recessive

(Horiuchi et al., 2003)

partially lethal (with lola^ORC4)

(Horiuchi et al., 2003)

partially lethal (with lola^ORE50)

(Horiuchi et al., 2003)

viable (with lola⁶²⁹)

(Bass et al., 2007)

viable (with lola^ORE119)

(Horiuchi et al., 2003)

Phenotype Manifest In

egg chamber | germline clone

(Bass et al., 2007)

larval longitudinal connective

(Crowner et al., 2002)

nurse cell | germline clone

(Bass et al., 2007)

Detailed Description

Statement

Reference

Females containing homozygous germline clones show defects in stage 14 egg chambers, including dumpless egg chambers (egg chambers in which the nurse cells have failed to transfer their cytoplasmic contents to the oocyte, have intact nuclei and do not appear to be undergoing programmed cell death) and egg chambers with persisting nurse cell nuclei (egg chambers in which nurse cells have transferred their cytoplasmic contents to the oocyte, but their nuclei have failed to undergo programmed cell death including nuclear condensation).

(Bass et al., 2007)

Fas2-expressing axons cross the midline in only 25% of homozygous stage 16/17 embryos, with one or two segments per embryo usually being affected.

(Crowner et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

lola^ORE120 has abnormal neuroanatomy phenotype, enhanceable by robo[+]/robo1³

(Crowner et al., 2002)

lola^ORE120 has abnormal neuroanatomy phenotype, enhanceable by sli²/sli[+]

(Crowner et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

lola^ORE120 has larval longitudinal connective phenotype, enhanceable by robo[+]/robo1³

(Crowner et al., 2002)

lola^ORE120 has larval longitudinal connective phenotype, enhanceable by sli²/sli[+]

(Crowner et al., 2002)

Enhancer of

Statement

Reference

lola^ORE120/lola[+] is an enhancer of wing margin phenotype of ct^53d

(Krupp et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

98% of lola^ORE120 sli²/lola^ORE120 embryos show Fas2-expressing axons crossing the midline. There are an average of 4.8 crossovers per affected embryo. 93% of lola^ORE120 robo³/lola^ORE120 embryos show Fas2-expressing axons crossing the midline. There are an average of 4.4 crossovers per affected embryo.