FlyBase Allele Report: Dmel\RanGAP[EP1173]

General Information

Symbol

Dmel\RanGAP^EP1173

Species

D. melanogaster

Name

FlyBase ID

FBal0130612

Feature type

allele

Associated gene

Dmel\RanGAP

Associated Insertion(s)

P{EP}RanGAP^EP1173

Carried in Construct

Also Known As

EP(2)1173

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}RanGAP^EP1173

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:19,445,136..19,445,136 [+]

(Dubey et al., 2024, Maybeck and Röper, 2009, Tanji et al., 2006, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | female limited | progressive | third instar larval stage

(Wu et al., 2019)

abnormal neuroanatomy | prepupal stage

(Wu et al., 2019)

abnormal neuroanatomy | prepupal stage (with RanGAP³²⁷)

(Wu et al., 2019)

abnormal neuroanatomy | progressive | third instar larval stage (with RanGAP³²⁷)

(Wu et al., 2019)

decreased cell number | female limited | progressive | third instar larval stage

(Wu et al., 2019)

decreased cell number | prepupal stage

(Wu et al., 2019)

decreased cell number | prepupal stage (with RanGAP³²⁷)

(Wu et al., 2019)

decreased cell number | progressive | third instar larval stage (with RanGAP³²⁷)

(Wu et al., 2019)

decreased occurrence of cell division | female | third instar larval stage

(Wu et al., 2019)

lethal - all die during pupal stage | female

(Wu et al., 2019)

lethal - all die during pupal stage | female (with RanGAP³²⁷)

(Wu et al., 2019)

lethal - all die during pupal stage | male (with RanGAP³²⁷)

(Wu et al., 2019)

partially lethal - majority die | male

(Wu et al., 2019)

partially lethal - majority live | maternal effect

(Virágh et al., 2012)

some die during embryonic stage | maternal effect

(Virágh et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

RanGAP^EP1173 homozygous third instar larvae display brain lobes with a normal glossy physical appearance, but there is a significant decrease in the number of central brain neuroblasts only in females, which does not show at second instar or early third instar (i.e. 48h and 72h after larval hatching, respectively), as compared to controls. At the pre-pupal stage (i.e. at 120h afer larval hatching) males also start showing a significant decrease in the number of central brain neuroblasts, as compared to controls. Female third instar neuroblasts show a severe decrease in the frequency of cell division (phospho-H3 and EdU stainings), despite of identical levels of apoptosis (Caspase 3 staining) in the brain.

Both male and female RanGAP^EP1173/RanGAP³²⁷ show a progressive decrease in the number of central brain neuroblasts from early third instar larval stage into prepupal stage (i.e. at 72h-120h after larval hatching, but not at 48h after larval hatching).

(Wu et al., 2019)

Expression via Scer\GAL4^fkh.PH results in strongly penetrant gland shape/lumen ('shepherd's crook') and positioning ('hook') phenotypes in the embryonic salivary gland.

(Maybeck and Röper, 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

RanGAP^EP1173 has increased mortality during development | maternal effect phenotype, enhanceable by Fs(2)Ket^RE34

(Virágh et al., 2012)

Suppressed by