FB2024_03 , released June 25, 2024
Allele: Dmel\MycPG45
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General Information
Symbol
Dmel\MycPG45
Species
D. melanogaster
Name
FlyBase ID
FBal0130426
Feature type
allele
Associated gene
Dmel\Myc
Associated Insertion(s)
P{GawB}MycPG45
Carried in Construct
Also Known As
dmPG45, PG45, dmycPG45
Key Links
Allele class

hypomorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - molecular evidence

(Benassayag et al., 2005)
Mutagen
Delta2-3 transposase
(Bourbon et al., 2002)
Progenitor genotype
Associated Insertion(s)
P{GawB}MycPG45
Cytology
Description

Insertion near the putative transcription start site.

(Benassayag et al., 2005)
Allele components
Component
Use(s)
Inserted element
P{GawB}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The majority of hemizygous males die early in the pupal stage. Development is delayed compared to wild-type controls.

      The wing discs of mutant third instar larvae are reduced in size by approximately 30% relative to wild type, due to a reduction in cell size.

      Homozygous clones are on average only approximately 10% of the size of their wild-type twin spot.

      (Benassayag et al., 2005)

      dm1/dmPG45 females produce eggs with a 2-3% hatching rate.

      (Moberg et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Expression of either Hsap\MYChs.1 or Hsap\MYChs.2 retards the lethal phase of dmPG45 males.

      Expression of Hsap\MYCScer\UAS.1 under the control of Scer\GAL4dm-PG45 fails to rescue the lethality of dmPG45 males (at either 18, 22 or 26[o]C).

      Expression of Hsap\MYCScer\UAS.2 under the control of Scer\GAL4dm-PG45 partially rescues the lethality of dmPG45 males. Surviving adult males have normal bristles, but are sterile. The size of the wings and of the wing cells is almost normal.

      Expression of Hsap\MYCScer\UAS.S under the control of Scer\GAL4dm-PG45 partially rescues the lethality of dmPG45 males. Surviving adult males are fertile, but have thin bristles. The size of the wings and of the wing cells is reduced approximately 15% compared to wild type.

      (Benassayag et al., 2005)
      Complementation and Rescue Data
      Rescued by

      MycPG45 is rescued by Scer\GAL4Myc-PG45/MycΔN.UAS.Tag:HA

      (Schwinkendorf and Gallant, 2009)

      MycPG45 is rescued by Scer\GAL4Myc-PG45/MycMB2A.UAS.Tag:HA

      (Schwinkendorf and Gallant, 2009)

      MycPG45 is rescued by Scer\GAL4Myc-PG45/MycΔMB2.UAS.Tag:HA

      (Schwinkendorf and Gallant, 2009)
      Partially rescued by

      MycPG45 is partially rescued by Mychs.PSA

      (Benassayag et al., 2005)
      Not rescued by

      MycPG45 is not rescued by Scer\GAL4Myc-PG45/MycWT.UAS.Tag:HA

      (Schwinkendorf and Gallant, 2009)

      MycPG45 is not rescued by Scer\GAL4Myc-PG45/MycΔMB3.UAS.Tag:HA

      (Schwinkendorf and Gallant, 2009)

      MycPG45 is not rescued by Scer\GAL4Myc-PG45/MycUAS.cZa

      (Benassayag et al., 2005)
      Comments

      dmhs.PSA partially rescues the lethality of dmPG45 mutants, with pharate adults being obtained.

      Expression of dmScer\UAS.cZa under the control of Scer\GAL4dm-PG45 fails to rescue the lethality of dmPG45 males (at either 18, 22 or 26[o]C).

      The reduced size of homozygous dmPG45 clones in the imaginal disc is completely suppressed if the clones are expressing Hsap\MYCScer\UAS.2 under the control of Scer\GAL4dm-PG45.

      The reduced size of homozygous dmPG45 clones in the imaginal disc is partially suppressed if the clones are expressing Hsap\MYCScer\UAS.S under the control of Scer\GAL4dm-PG45 (the double mutant clones are approximately 70% the size of the wild-type twin spots).

      (Benassayag et al., 2005)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Re-mobilisation of the transposon causes the lethality phenotype to be lost.

      (Bourbon et al., 2002)

      Precise excision of the insertion reverts the lethal phenotype.

      (Benassayag et al., 2005)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      MycPG45
      dmPG45
      (Baumgardt et al., 2014, Schwinkendorf and Gallant, 2009, Hulf et al., 2005)
      dmycPG45
      (Rhiner et al., 2009, Benassayag et al., 2005)
      mycPG45
      (Moreno and Basler, 2004)
      Name Synonyms
      Secondary FlyBase IDs
        References (9)