FlyBase Allele Report: Dmel\Ptp52F[18.3]

General Information

Symbol

Dmel\Ptp52F^18.3

Species

D. melanogaster

Name

FlyBase ID

FBal0128128

Feature type

allele

Associated gene

Dmel\Ptp52F

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schindelholz et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: S941P.

(Schindelholz et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:16,173,022..16,173,022 [-]

Nucleotide change:

T16173022C

Amino acid change:

S940P | Ptp52F-PB

Reported amino acid change:

S941P

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement (Figure 1 sequence).

(Schindelholz et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

central nervous system

(Schindelholz et al., 2001)

central nervous system (with Df(2R)Jp4)

(Schindelholz et al., 2001)

gastric caecum | prepupal stage

(Santhanam et al., 2014)

gastric caecum | prepupal stage (with Ptp52F^8.10.3)

(Santhanam et al., 2014)

larval abdominal segmental nerve

(Schindelholz et al., 2001)

larval abdominal segmental nerve (with Df(2R)Jp4)

(Schindelholz et al., 2001)

larval abdominal segmental nerve (with Df(2R)Jp8)

(Schindelholz et al., 2001)

larval intersegmental nerve

(Schindelholz et al., 2001)

larval intersegmental nerve (with Df(2R)Jp4)

(Schindelholz et al., 2001)

larval intersegmental nerve (with Df(2R)Jp8)

(Schindelholz et al., 2001)

larval RP5 motor neuron

(Schindelholz et al., 2001)

larval RP5 motor neuron (with Df(2R)Jp4)

(Schindelholz et al., 2001)

larval RP5 motor neuron (with Df(2R)Jp8)

(Schindelholz et al., 2001)

larval VL3/4 motor neuron

(Schindelholz et al., 2001)

larval VL3/4 motor neuron (with Df(2R)Jp4)

(Schindelholz et al., 2001)

larval VL3/4 motor neuron (with Df(2R)Jp8)

(Schindelholz et al., 2001)

larval VO1 motor neuron

(Schindelholz et al., 2001)

larval VO1 motor neuron (with Df(2R)Jp4)

(Schindelholz et al., 2001)

larval VO1 motor neuron (with Df(2R)Jp8)

(Schindelholz et al., 2001)

larval VO2 motor neuron

(Schindelholz et al., 2001)

larval VO2 motor neuron (with Df(2R)Jp4)

(Schindelholz et al., 2001)

larval VO2 motor neuron (with Df(2R)Jp8)

(Schindelholz et al., 2001)

midgut | prepupal stage

(Santhanam et al., 2014)

midgut | prepupal stage (with Ptp52F^8.10.3)

(Santhanam et al., 2014)

motor neuron

(Ratnaparkhi and Zinn, 2007)

pCC neuron

(Schindelholz et al., 2001)

Detailed Description

Statement

Reference

Ptp52F^18.3 heterozygotes and, more severely, Ptp52F^18.3/Ptp52F^8.10.3 transheterozygotes exhibit a significant decrease in the apoptosis levels in gastric caecum cells, and delays in both the histolysis of larval midgut and the removal of the gastric caeca during metamorphosis, as compared to controls.

(Santhanam et al., 2014)

Ptp52F^18.3 mutants exhibit defects in motor neuron projection in late stage 16 embryos.

(Ratnaparkhi and Zinn, 2007)

Homozygous mutants, and hemizygous mutants (over Df(2R)Jp4 or Df(2R)Jp8) show a range of phenotypes in the SNa branch of the segmental nerve. Additional branches are seen at random position, either the posterior of anterior branch is missing, or the SNa stalls near the bifurcation point. Ptp52F^18.3/Df(2R)Jp8 mutant embryos also occasionally have completely missing SNa branches. In homozygous mutant embryos, growth cones of the pCC neuron that should grow onto lateral G branch 5 (LG5) stalls. AT later stages the longitudinal pathway is thin in 50% of segments, and the connection between the pCC and the pathway appears to be missing in some hemisegments. By stage 15, however, the medial 1D4 positive bundle (which contains the pCC axon) has a relatively normal appearance. Homozygous mutants, and hemizygous mutants (over Df(2R)Jp4 or Df(2R)Jp8) show a weak phenotype in the ISNb nerve branch. ISNb stalls in the VLM field, or shows a bypass phenotype at a low frequency. Homozygous mutants, and hemizygous mutants (over Df(2R)Jp4 or Df(2R)Jp8) show a range of phenotypes in the Intersegmental nerve (ISN). ISNs sometimes terminate at the first branchpoint, or more frequently terminate at the second branchpoint, and occasionally ISN appear to reach the terminal arbor position, but are thin or bifurcated.

(Schindelholz et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ptp52F^18.3 has abnormal neuroanatomy phenotype, enhanceable by Ptp4E¹/Ptp10D¹

(Jeon et al., 2008)

NOT Enhanced by

Statement

Reference

Ptp52F^18.3 has abnormal neuroanatomy phenotype, non-enhanceable by Ptp4E¹

(Jeon et al., 2008)

Suppressor of

Statement

Reference

Ptp52F^18.3 is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, Scer\GAL4^elav.PLu, fog^UAS.cRa

(Ratnaparkhi and Zinn, 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ptp52F^18.3 has motor neuron phenotype, enhanceable by Ptp4E¹/Ptp10D¹

(Jeon et al., 2008)

Ptp52F^18.3 has larval abdominal segmental nerve phenotype, enhanceable by Ptp10D¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VO2 motor neuron phenotype, enhanceable by Ptp10D¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval intersegmental nerve phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VL3/4 motor neuron phenotype, enhanceable by Ptp10D¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VO1 motor neuron phenotype, enhanceable by Ptp10D¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval RP5 motor neuron phenotype, enhanceable by Ptp10D¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval intersegmental nerve phenotype, enhanceable by Ptp10D¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VO2 motor neuron phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VL3/4 motor neuron phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VO1 motor neuron phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval RP5 motor neuron phenotype, enhanceable by Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

NOT Enhanced by

Statement

Reference

Ptp52F^18.3 has motor neuron phenotype, non-enhanceable by Ptp4E¹

(Jeon et al., 2008)

Ptp52F^18.3 has larval VO2 motor neuron phenotype, non-enhanceable by Ptp99A¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VL3/4 motor neuron phenotype, non-enhanceable by Ptp99A¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VO1 motor neuron phenotype, non-enhanceable by Ptp99A¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval RP5 motor neuron phenotype, non-enhanceable by Ptp99A¹

(Schindelholz et al., 2001)

Suppressed by

Statement

Reference

Ptp52F^18.3 has central nervous system phenotype, suppressible by Lar^5.5

(Schindelholz et al., 2001)

NOT suppressed by

Statement

Reference

Ptp52F^18.3 has larval VO2 motor neuron phenotype, non-suppressible by Ptp99A¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VL3/4 motor neuron phenotype, non-suppressible by Ptp99A¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval VO1 motor neuron phenotype, non-suppressible by Ptp99A¹

(Schindelholz et al., 2001)

Ptp52F^18.3 has larval RP5 motor neuron phenotype, non-suppressible by Ptp99A¹

(Schindelholz et al., 2001)

Enhancer of

Statement

Reference

Ptp52F^18.3 is an enhancer of larval VL3/4 motor neuron phenotype of Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 is an enhancer of larval VO1 motor neuron phenotype of Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 is an enhancer of larval RP5 motor neuron phenotype of Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 is an enhancer of larval intersegmental nerve phenotype of Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Ptp52F^18.3 is an enhancer of larval VO2 motor neuron phenotype of Ptp69D¹/Df(3L)8ex25

(Schindelholz et al., 2001)

Suppressor of

Statement

Reference

Ptp52F[+]/Ptp52F^18.3 is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Ptp52F[+]/Ptp52F^18.3 is a suppressor of wing | male limited phenotype of Scer\GAL4^Bx-MS1096, fog^UAS.cRa

(Ratnaparkhi, 2013)

Ptp52F^18.3 is a suppressor of embryonic/larval central nervous system phenotype of Scer\GAL4^elav-C155, Scer\GAL4^elav.PLu, fog^UAS.cRa

(Ratnaparkhi and Zinn, 2007)

Ptp52F^18.3 is a suppressor of larval segmental nerve branch SNa of A1-7 phenotype of Scer\GAL4^elav-C155, Scer\GAL4^elav.PLu, fog^UAS.cRa

(Ratnaparkhi and Zinn, 2007)

Ptp52F^18.3 is a suppressor of fascicle phenotype of Scer\GAL4^elav-C155, Scer\GAL4^elav.PLu, fog^UAS.cRa

(Ratnaparkhi and Zinn, 2007)

Other

Statement

Reference

Ptp10D¹, Ptp4E¹, Ptp52F^18.3 has abdominal nerve phenotype

(Jeon et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The presence of Ptp52F^18.3 strongly suppresses the wing defects seen in male flies expressing fog^Scer\UAS.cRa under the control of Scer\GAL4^Bx-MS1096.

(Ratnaparkhi, 2013)

Ptp4E¹ Ptp52F^18.3 double mutants have motor axon phenotypes indistinguishable from Ptp52F^18.3 single mutants.

Ptp4E¹ Ptp10D¹ Ptp52F^18.3 triple mutants have stronger motor axon phenotypes than Ptp52F^18.3 single mutants. The triple mutants also exhibit an enhancement of the ISN truncation phenotype seen at the second branchpoint in Ptp10D¹ Ptp52F^18.3 double mutants.

(Jeon et al., 2008)

Removal of Ptp52F function in fog^Scer\UAS.cRa overexpression mutants, through a Ptp52F^18.3 mutant background, results in a strong suppression of the CNS phenotype. Only 3% of segments exhibit ectopic midline crossing, as compared to 44% for fog^Scer\UAS.cRa overexpression (under the control of Scer\GAL4^elav-C155 and Scer\GAL4^elav.PLu) in a wild-type background. For embryos with one copy of the driver (Scer\GAL4^elav.PLu), the phenotype was suppressed from 27% to 5%. The longitudinal tracts appear normal in fog^Scer\UAS.cRa, Ptp52F^18.3 embryos, resembling those seen in Ptp52F^18.3 single mutants.

(Ratnaparkhi and Zinn, 2007)

The addition of Ptp10D¹ enhances the SNa and ISNb phenotypes seen in Ptp52F^18.3 homozygotes alone. An ISNb stall phenotype is greatly enhanced in these embryos. The addition of Lar^5.5 enhances the SNa phenotype seen in Ptp52F^18.3 homozygotes alone. The addition of Ptp69D¹/Df(3L)8ex25 enhances the SNa phenotype seen in Ptp52F^18.3 homozygotes alone. The addition of Ptp99A¹ doesn't enhance the strength of the SNa phenotype seen in Ptp52F^18.3 homozygotes, but does decrease the penetrance of the ectopic branch phenotype. The addition of Ptp52F^18.3 to Ptp69D¹/Df(3L)8ex25 mutant embryos strongly enhances the ISNb phenotype seen. The addition of Ptp99A¹ has no effect on the Ptp52F^18.3 ISNb phenotype. The addition of Ptp10D¹ strongly enhances the ISN phenotypers seen in Ptp52F^18.3 mutants alone. The addition of Ptp52F^18.3 to Ptp69D¹/Df(3L)8ex25 mutant embryos strongly enhances the ISN phenotype seen. The central nervous system phenotype seen in Ptp52F^18.3 embryos is suppressed by the addition of Lar^5.5.

(Schindelholz et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6590

Ptp52F^18.3/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ptp52F^18.3

(Santhanam et al., 2014, Jeon et al., 2008, Ratnaparkhi and Zinn, 2007)

ptp52F^18.3

(Ratnaparkhi, 2013)

Name Synonyms

Secondary FlyBase IDs

References (5)

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