FlyBase Allele Report: Dmel\robo2[EP2582]

General Information

Symbol

Dmel\robo2^EP2582

Species

D. melanogaster

Name

FlyBase ID

FBal0121553

Feature type

allele

Associated gene

Dmel\robo2

Associated Insertion(s)

P{EP}robo2^EP2582

Carried in Construct

Also Known As

EP2582, EP(2)2582, P{EP}lea^EP2582

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(McGovern et al., 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Simpson et al., 2000, Simpson et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}robo2^EP2582

Cytology

Description

The P{EP} element is inserted 101 nucleotides 5' to the predicted mRNA start.

(Kraut et al., 2001)

P{EP} insertion upstream of lea.

(Simpson et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:1,420,531..1,420,531 [-]

(Koch et al., 2017, Sasse and Klämbt, 2016, Kavi et al., 2015, Sanhueza et al., 2015, Zhu et al., 2005, Couch et al., 2004, Jhaveri et al., 2004, Kraut and Zinn, 2004, McGovern et al., 2003, Gene Disruption Project members, 2001-, Kraut et al., 2001, Kraut et al., 2001, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Simpson et al., 2000, Simpson et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

ameliorates inflammatory bowel disease 10

modeled by Atg16^d67

(Nagy et al., 2017)

modeled by Atg16^MI00187

(Nagy et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^48Y

(Kraut and Zinn, 2004)

abnormal neuroanatomy, with Scer\GAL4^ato.3.6

(Kraut and Zinn, 2004)

abnormal neuroanatomy, with Scer\GAL4^elav-C155

(Kraut and Zinn, 2004)

lethal, with Scer\GAL4^insc-Mz1407

(Rajagopalan et al., 2000)

partially lethal - majority die, with Scer\GAL4^sca-4512

(McGovern et al., 2003)

Phenotype Manifest In

abdominal lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^48Y

(Kraut and Zinn, 2004)

abdominal lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^ato.3.6

(Kraut and Zinn, 2004)

abdominal lateral pentascolopidial chordotonal organ lch5, with Scer\GAL4^elav-C155

(Kraut and Zinn, 2004)

abdominal lateral pentascolopidial chordotonal organ lch5 & scolopidial dendrite, with Scer\GAL4^elav-C155

(Kraut and Zinn, 2004)

abdominal lateral pentascolopidial chordotonal organ lch5 & scolopidial dendritic cap cell, with Scer\GAL4^elav-C155

(Kraut and Zinn, 2004)

adult antennal lobe commissure, with Scer\GAL4^SG18.1

(Jhaveri et al., 2004)

antennal lobe glomerulus, with Scer\GAL4^SG18.1

(Jhaveri et al., 2004)

cell body & serotonin neuron, with Scer\GAL4^eg-Mz360

(Couch et al., 2004)

dMP2 neuron, with Scer\GAL4^15J2

(Simpson et al., 2000)

larval intersegmental nerve, with Scer\GAL4^elav-C155

(Kraut et al., 2001)

larval ventral nerve cord commissure, with Scer\GAL4^elav.PLu

(Rajagopalan et al., 2000)

larval ventral nerve cord commissure, with Scer\GAL4^sca-4512

(McGovern et al., 2003)

serotonin neuron & ventral nerve cord, with Scer\GAL4^eg-Mz360

(Couch et al., 2004)

vMP2 neuron, with Scer\GAL4^15J2

(Simpson et al., 2000)

Detailed Description

Statement

Reference

In lea^EP2582; Scer\GAL4^eg-Mz360 embryos, serotonergic axons do not cross the midline. Despite their defects in serotonergic neuronal axon guidance, these neurons retain their normal ability to take up serotonin. Unlike wild-type serotonergic neurons, the ability of serotonergic neurons in the ventral nerve cord of stage 16 lea^EP2582; Scer\GAL4^eg-Mz360 embryos to take up serotonin is not lost when their axonal contacts with the midline are severed. The cell bodies of serotonergic neurons in the ventral nerve cord of stage 16 lea^EP2582; Scer\GAL4^eg-Mz360 embryos are shifted laterally compared to wild-type.

(Couch et al., 2004)

Antennal commissure formation is abolished and glomerular morphology is disrupted in the antennal lobes of lea^EP2582; Scer\GAL4^SG18.1 flies.

(Jhaveri et al., 2004)

Abdominal lch5 chordotonal organs are transformed so that they resemble thoracic dch3 chordotonal organs in larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155. The transformed "lch5" chordotonal organs are displaced dorsally in the ectoderm. They have the usual number of five neurons per cluster, but their dendrites and support cells show a variety of orientations, which range from the ventral orientation typical of dch3 organs, through intermediate bent orientations, to orientations similar to those of normal lch5 organs. At 29^oC, 44% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology. At 25^oC, 32% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology. Defects indicating transformation of the lch5 chordotonal organs can be detected as early as stage 14 in embryos expressing lea^EP2582 under the control of Scer\GAL4^elav-C155. The cap cells of the transformed "lch5" organs always follow the orientation of the transformed neurons. At 29^oC, 30.5% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in larvae expressing lea^EP2582 under the control of Scer\GAL4^ato.3.6. Some abdominal lch5 chordotonal organs fail to migrate ventrally (as occurs in wild type) in embryos expressing lea^EP2582 under the control of Scer\GAL4^ato.3.6. Some of these "stalled" abdominal clusters extend projections dorsally. At 29^oC, 17% of abdominal segments have partial or complete transformation of lch5 chordotonal organ morphology towards that of the thoracic dch3 chordotonal organs, in embryos expressing lea^EP2582 under the control of Scer\GAL4^48Y.

(Kraut and Zinn, 2004)

ventral nerve cord commissures in stage 17 lea^EP2582; Scer\GAL4^sca-4512 embryos are missing or fused.

(McGovern et al., 2003)

Mutant larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155 have ISN neuron pathfinding defects.

(Kraut et al., 2001)

When expression of lea^EP2582 is driven by Scer\GAL4^elav.PLu, commissures are lost.

(Rajagopalan et al., 2000)

Expression of lea^EP2582 under the control of Scer\GAL4^ap-md544 causes ap-expressing neurons to move laterally (relative to wild type) and extend anteriorly in a specific location between the intermediate and lateral Fas2-expressing pathways. The ap-expressing axons from neighbouring segments appear to pick the same lateral pathway and to fasciculate together as they extend anteriorly from segment to segment. Expression of lea^EP2582 under the control of Scer\GAL4^15J2 results in a bimodal phenotype. The dMP2 and vMP2 axons always appear to extend in a Fas2-expressing pathway, but they are now found in either the intermediate or lateral pathways (they normally extend in the medial pathway). They are usually found in the intermediate pathway and occasionally found in the lateral pathway.

(Simpson et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, robo2^EP2582 has abnormal neuroanatomy phenotype, suppressible | partially by sli²/sli[+]

(Kraut and Zinn, 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, robo2^EP2582 has abnormal neuroanatomy phenotype, non-suppressible by robo1⁷

(Kraut and Zinn, 2004)

NOT Enhancer of

Statement

Reference

robo2^EP2582, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

robo2^EP2582, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Cdc42^L89.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

robo2^EP2582/Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Suppressor of

Statement

Reference

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of abnormal cell number | adult stage phenotype of Atg16^d67

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of abnormal immune response | adult stage phenotype of Atg16^d67

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of abnormal cell number | adult stage phenotype of Atg16^MI00187

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of abnormal immune response | adult stage phenotype of Atg16^MI00187

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

robo2^EP2582, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

NOT Suppressor of

Statement

Reference

robo2^EP2582, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

robo2^EP2582, Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Cdc42^L89.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

robo2^EP2582/Scer\GAL4^GMR.PF is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^SG18.1, robo2^EP2582 has adult antennal lobe commissure phenotype, suppressible by Df(2R)WMG

(Jhaveri et al., 2004)

Scer\GAL4^SG18.1, robo2^EP2582 has antennal lobe glomerulus phenotype, suppressible by Df(2R)WMG

(Jhaveri et al., 2004)

Scer\GAL4^elav-C155, robo2^EP2582 has abdominal lateral pentascolopidial chordotonal organ lch5 phenotype, suppressible | partially by sli²/sli[+]

(Kraut and Zinn, 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav-C155, robo2^EP2582 has abdominal lateral pentascolopidial chordotonal organ lch5 phenotype, non-suppressible by robo1⁷

(Kraut and Zinn, 2004)

NOT Enhancer of

Statement

Reference

robo2^EP2582/Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

Suppressor of

Statement

Reference

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of adult posterior midgut epithelium phenotype of Atg16^d67

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of enteroendocrine cell | adult stage phenotype of Atg16^d67

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of enteroblast | adult stage phenotype of Atg16^d67

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of adult posterior midgut epithelium phenotype of Atg16^MI00187

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of enteroendocrine cell | adult stage phenotype of Atg16^MI00187

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^esg.PU is a suppressor of enteroblast | adult stage phenotype of Atg16^MI00187

(Nagy et al., 2017)

robo2^EP2582/Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

robo2^EP2582, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pvr^λ.UAS, Scer\GAL4^GMR.PF

(Zhu et al., 2005)

robo2^EP2582/robo2^EP2582 is a suppressor of serotonin neuron & ventral nerve cord phenotype of eg^Mz360

(Couch et al., 2004)

NOT Suppressor of

Statement

Reference

robo2^EP2582/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl::Egfr^λ.UAS

(Zhu et al., 2005)

robo2^EP2582/Scer\GAL4^elav.PLu is a non-suppressor of larval ventral nerve cord phenotype of sli²

(Rajagopalan et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The expression of robo2^EP2582 under the control of Scer\GAL4^esg.PU fully or partially suppresses the increased proportion of pre-enteroendocrine cells and the reciprocal decreased proportion of mature enteroendocrine cells, respectively, observed in the adult gut of Atg16^d67 or Atg16^MI00187 homozygotes; this expression also suppresses the severely decreased lifespan of Atg16^d67 or Atg16^MI00187 homozygotes upon oral infection with Pseudomonas aeruginosa or Enterococcus faecalis.

(Nagy et al., 2017)

Commissure formation and glomerular morphology in the antennal lobes of lea^EP2582; Scer\GAL4^SG18.1 flies are restored by Df(2R)WMG/+.

(Jhaveri et al., 2004)

The transformation of abdominal lch5 chordotonal organs to a morphology resembling thoracic dch3 chordotonal organs that is seen in larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155 is still seen if they are also mutant for robo⁷; 36.4% of abdominal segments have partial or complete transformation of the lch5 organs in the double mutant larvae. The transformation of abdominal lch5 chordotonal organs to a morphology resembling thoracic dch3 chordotonal organs that is seen in larvae expressing lea^EP2582 under the control of Scer\GAL4^elav-C155 is attenuated if the larvae also carry one copy of sli²; only 17.2% of abdominal segments have partial or complete transformation of the lch5 organs in the double mutant larvae at 25^oC.

(Kraut and Zinn, 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17071

w¹¹¹⁸; P{EP}robo2^EP2582

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

lea^EP2582

robo2^EP2582

(Sasse and Klämbt, 2016, Kraut et al., 2001, Kraut et al., 2001, Rajagopalan et al., 2000, Rajagopalan et al., 2000, Simpson et al., 2000, Simpson et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (17)

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