FlyBase Allele Report: Dmel\sns[XB3]

General Information

Symbol

Dmel\sns^XB3

Species

D. melanogaster

Name

FlyBase ID

FBal0117349

Feature type

allele

Associated gene

Dmel\sns

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Bour et al., 2000)

Progenitor genotype

Cytology

Description

Nucleotide substitution: G?A. The lesion creates a novel 3' splice acceptor site within an intron and leads to incorrect splicing of the transcript, which introduces a stop codon 67bp from the splice junction.

(Bour et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:8,830,819..8,830,819 [+]

Nucleotide change:

G8830819A

Reported nucleotide change:

G?A

Comment:

The mutation creates a novel 3' splice acceptor site within an intron and leads to incorrect splicing of the transcript, which introduces a stop codon 67bp from the splice junction.

(Bour et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nephrotic syndrome type 1

CEA with hbs²⁵⁹³

(Zhuang et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dorsal pharyngeal muscle cell

(Haralalka et al., 2014)

embryonic garland cell

(Weavers et al., 2009)

embryonic myoblast

(Richardson et al., 2007)

embryonic myoblast (with sns^ZF1.4)

(Kocherlakota et al., 2008, Galletta et al., 2004)

muscle cell of A1-7 dorsal acute muscle 1 | embryonic stage 15

(Menon et al., 2005)

muscle founder cell | embryonic stage

(Shelton et al., 2009)

presumptive embryonic/larval muscle system

(Menon et al., 2005)

Detailed Description

Statement

Reference

Mutant stage 16 embryos show a severe myoblast fusion defect in the dorsal pharyngeal muscle.

(Haralalka et al., 2014)

The eve-expressing DA1 founder cell, the Kr-expressing DO1 founder cell, and the nau-expressing VA1 founder cell undergo limited fusion in sns^XB3 embryos to generate bi- or tri-nucleate syncitia.

(Shelton et al., 2009)

Mutant embryonic garland cells lack diaphragms and lacunae, and instead the nephrocyte surface contains frequent, small patches of electron-dense subcortical material.

(Weavers et al., 2009)

sns^XB3 embryos do not exhibit actin foci in founder cell-fusion competent myoblast development, leading to defects in fusion.

(Richardson et al., 2007)

The extent of myoblast fusion in sns^XB3 mutant embryos is significantly reduced compared to wild type.

(Menon et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

sns^XB3 has muscle founder cell | embryonic stage phenotype, enhanceable by hbs²⁵⁹³

(Shelton et al., 2009)

NOT suppressed by

Statement

Reference

hbs²⁵⁹³, sns^XB3 has muscle founder cell | embryonic stage phenotype, non-suppressible by Scer\GAL4^Mef2.PR/sns^{20-5.UAS.Tag:HA}

(Shelton et al., 2009)

hbs²⁵⁹³, sns^XB3 has muscle founder cell | embryonic stage phenotype, non-suppressible by hbs^{ΔICD.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Shelton et al., 2009)

sns^XB3/sns^ZF1.4 has embryonic myoblast phenotype, non-suppressible by Scer\GAL4^how-24B/sns^{UAS.Tag:GPI(Fas1)}

(Galletta et al., 2004)

Enhancer of

Statement

Reference

sns[+]/sns^XB3 is an enhancer of embryonic myoblast phenotype of hbs⁴⁵⁹/hbs²⁵⁹³

(Shelton et al., 2009)

Suppressor of

Statement

Reference

sns^XB3 is a suppressor of embryonic myoblast phenotype of hbs⁴⁵⁹/hbs²⁵⁹³

(Artero et al., 2001)

sns^XB3 is a suppressor of embryonic midgut chamber 1 phenotype of hbs⁴⁵⁹/hbs²⁵⁹³

(Artero et al., 2001)

sns^XB3 is a suppressor of visceral muscle primordium phenotype of hbs⁴⁵⁹/hbs²⁵⁹³

(Artero et al., 2001)

sns^XB3 is a suppressor of presumptive embryonic/larval muscle system phenotype of hbs⁴⁵⁹/hbs²⁵⁹³

(Artero et al., 2001)

Other

Statement

Reference

hbs⁴⁵⁹/hbs²⁵⁹³, sns^XB3/sns^D1 has muscle founder cell | embryonic stage phenotype

(Shelton et al., 2009)

hbs²⁵⁹³, sns^XB3 has embryonic garland cell phenotype

(Zhuang et al., 2009)

hbs²⁵⁹³, sns^XB3 has muscle cell of A1-7 dorsal acute muscle 1 | embryonic stage 15 phenotype

(Menon et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

There is a lower number of eve-expressing DA1 founder cells, Kr-expressing DO1 founder cells, and nau-expressing VA1 founder cells in sns^XB3 hbs²⁵⁹³ double mutant embryos than in sns^XB3 embryos - these founder cells remain mononucleate in most hemi-segments.

sns^XB3, hbs²⁵⁹³ / sns^D1, hbs⁴⁵⁹ embryos have a relatively low number of eve-expressing DA1 founder cells - fewer than in sns^XB3 embryos.

The limited number of unfused myoblasts in hbs²⁵⁹³/hbs⁴⁵⁹ embryos increases when embryos are also heterozygous for sns^XB3.

Scer\GAL4^Mef2.PR-mediated expression of sns^{20-5.Scer\UAS.T:Ivir\HA1} or hbs^{ΔICD.Scer\UAS.T:Ivir\HA1} fails to rescue formation of eve-positive DA1 or Kr-positive DO1 bi- and tri-nucleate muscle precursors in sns^XB3, hbs²⁵⁹³ double mutant embryos.

(Shelton et al., 2009)

Almost 60% of garland cell nephrocytes (GCNs) remain mononucleate at early stage 16 in sns^XB3 hbs²⁵⁹³ double mutant embryos (in contrast to wild-type embryos where 99.6% of the GCNs are binucleate at this stage). The GCNs of stage 16 sns^XB3 hbs²⁵⁹³ double mutant embryos are disorganised, misshapen and more loosely associated than in wild-type embryos.

(Zhuang et al., 2009)

The extent of myoblast fusion in hbs²⁵⁹³; sns^XB3 embryos is significantly reduced compared to wild type.

(Menon et al., 2005)

Expression of Fas1::sns^GPI.Scer\UAS under the control of Scer\GAL4^how-24B fails to rescue the mutant phenotype of sns^ZF1.4/sns^XB3 embryos. Fusion competent myoblasts do not appear to migrate towards the founder cells in these embryos.

(Galletta et al., 2004)

Both the gut and the somatic mesoderm phenotypers seen in hbs⁴⁵⁹/hbs²⁵⁹³ embryos are suppressed by the addition of sns^XB3.

(Artero et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sns^43-49

(Bour et al., 2000)

Rescued by

sns^XB3/sns^ZF1.4 is rescued by Scer\GAL4^Mef2.PR/sns^UAS.Tag:HA

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by Scer\GAL4^Mef2.PR/sns^{5xPDZ.UAS.Tag:HA}

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by sns^{A17.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by sns^{Δ1279-1482.UAS}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by sns^{Δ1113-1163.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by sns^{Δ1164-1232.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by Scer\GAL4^Mef2.PR/sns^{Δ1233-1263.UAS.Tag:HA}

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by sns^{Δ1263-1278.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by Scer\GAL4^Mef2.PR/sns^{F6.UAS.Tag:HA}

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by sns^{F11.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is rescued by Scer\GAL4^how-24B/sns^UAS.cGa

(Galletta et al., 2004)

Partially rescued by

sns^XB3/sns^ZF1.4 is partially rescued by Scer\GAL4^Mef2.PR/sns^{Δ1113-1232.UAS.Tag:HA}

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is partially rescued by sns^{Δ1164-1278.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is partially rescued by sns^{F14.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is partially rescued by Scer\GAL4^Mef2.PR/sns^2xPXXP.UAS

(Kocherlakota et al., 2008)

Not rescued by

sns^XB3/sns^ZF1.4 is not rescued by Scer\GAL4^Mef2.PR/sns^{20-5.UAS.Tag:HA}

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is not rescued by Scer\GAL4^Mef2.PR/sns^{Δ1113-1278.UAS.Tag:HA}

(Kocherlakota et al., 2008)

sns^XB3/sns^ZF1.4 is not rescued by sns^{ΔΔ1113-1163.1233-1278.UAS.Tag:HA}/Scer\GAL4^Mef2.PR

(Kocherlakota et al., 2008)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92191

w^*; sns^XB3 P{neoFRT}42D/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sns^XB3

(Carrasco-Rando et al., 2023, Deng et al., 2015, Bothe et al., 2014, Haralalka et al., 2014, Shelton et al., 2009, Weavers et al., 2009, Zhuang et al., 2009, Kocherlakota et al., 2008, Richardson et al., 2007, Menon et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (14)

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