FlyBase Allele Report: Dmel\hbs[459]

General Information

Symbol

Dmel\hbs⁴⁵⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0130216

Feature type

allele

Associated gene

Dmel\hbs

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Johnson et al., 2008)

Mutagen

ethyl methanesulfonate

(Artero et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: K554term.

(Artero et al., 2001)

Nucleotide substitution: A?T.

(Artero et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,038,168..15,038,168 [+]

Nucleotide change:

A15038168T

Reported nucleotide change:

A?T

Amino acid change:

K553term | hbs-PA; K553term | hbs-PB; K553term | hbs-PC

Reported amino acid change:

K554term

(Artero et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity (with hbs^VDRC.cUa), with Scer\GAL4^hs.2sev

(Singh and Mlodzik, 2012)

visible (with Df(2R)ED2423)

(Singh and Mlodzik, 2012)

visible (with hbs¹)

(Grillo-Hill and Wolff, 2009)

visible (with hbs⁶⁶)

(Grillo-Hill and Wolff, 2009)

Phenotype Manifest In

embryonic midgut chamber 1 (with Df(2R)X28)

(Artero et al., 2001)

embryonic midgut chamber 1 (with hbs²⁵⁹³)

(Artero et al., 2001)

embryonic myoblast (with Df(2R)X28)

(Artero et al., 2001)

embryonic myoblast (with hbs²⁵⁹³)

(Shelton et al., 2009, Artero et al., 2001)

eye (with hbs¹)

(Grillo-Hill and Wolff, 2009)

eye (with hbs⁶⁶)

(Grillo-Hill and Wolff, 2009)

eye (with hbs^VDRC.cUa), with Scer\GAL4^hs.2sev

(Singh and Mlodzik, 2012)

notal microchaeta (with Df(2R)ED2423)

(Singh and Mlodzik, 2012)

ommatidium, with Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

photoreceptor cell R3, with Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

photoreceptor cell R7, with Scer\GAL4^hs.2sev, hbs^VDRC.cUa

(Singh and Mlodzik, 2012)

photoreceptor cell R8 (with hbs⁶⁶)

(Tan et al., 2020)

photoreceptor cell R8 (with hbs³⁶¹)

(Tan et al., 2020)

photoreceptor cell R8 (with hbs¹¹³⁰)

(Tan et al., 2020)

photoreceptor cell R8 (with hbs²⁵⁹³)

(Tan et al., 2020)

presumptive embryonic/larval muscle system (with Df(2R)X28)

(Artero et al., 2001)

presumptive embryonic/larval muscle system (with hbs²⁵⁹³)

(Artero et al., 2001)

visceral muscle primordium (with Df(2R)X28)

(Artero et al., 2001)

visceral muscle primordium (with hbs²⁵⁹³)

(Artero et al., 2001)

Detailed Description

Statement

Reference

hbs⁴⁵⁹/hbs⁶⁶, hbs⁴⁵⁹/hbs³⁶¹, hbs⁴⁵⁹/hbs¹¹³⁰ or hbs⁴⁵⁹/hbs²⁵⁹³ eyes exhibit reduced number of Rh5 expressing R8 cells.

(Tan et al., 2020)

hbs⁴⁵⁹/+ dominantly enhances the Scer\GAL4^hs.2sev>hbs^VDRC.cUa-induced planar cell polarity phenotype in the eye.

hbs⁴⁵⁹/Df(2R)ED2423 mutants display a reduction in the number of notal microchaetae compared with wild-type.

(Singh and Mlodzik, 2012)

hbs⁶⁶/hbs⁴⁵⁹ and hbs¹/hbs⁴⁵⁹ adults have rough eyes.

(Grillo-Hill and Wolff, 2009)

hbs²⁵⁹³/hbs⁴⁵⁹ embryos exhibit a limited number of unfused myoblasts.

(Shelton et al., 2009)

Stage 12 hbs⁴⁵⁹/Df(2R)X28 or hbs⁴⁵⁹/hbs²⁵⁹³ embryos have a reduction in the number of visceral muscle progenitors. hbs⁴⁵⁹/Df(2R)X28, or hbs⁴⁵⁹/hbs²⁵⁹³ embryos The overall pattern or somatic muscles is normal, though there is a partial fusion block in embryos (especially around the heart). The unfused myoblasts can still extend filopodia. Mutant embryos also show a gut phenotype consisting of an enlarged first gut chamber, which pushes the dorsal muscles and heart upwards.

(Artero et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

hbs⁴⁵⁹ is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

hbs⁴⁵⁹/Df(2R)ED2423 has notal microchaeta phenotype, enhanceable by Psn⁹/Psn¹⁴³

(Singh and Mlodzik, 2012)

hbs⁴⁵⁹/hbs²⁵⁹³ has embryonic myoblast phenotype, enhanceable by sns[+]/sns^XB3

(Shelton et al., 2009)

hbs⁴⁵⁹/hbs²⁵⁹³ has embryonic myoblast phenotype, enhanceable by sns[+]/sns^D1

(Shelton et al., 2009)

Suppressed by

Statement

Reference

hbs⁴⁵⁹/hbs²⁵⁹³ has embryonic myoblast phenotype, suppressible by sns^XB3

(Artero et al., 2001)

hbs⁴⁵⁹/hbs²⁵⁹³ has embryonic midgut chamber 1 phenotype, suppressible by sns^XB3

(Artero et al., 2001)

hbs⁴⁵⁹/hbs²⁵⁹³ has visceral muscle primordium phenotype, suppressible by sns^XB3

(Artero et al., 2001)

hbs⁴⁵⁹/hbs²⁵⁹³ has presumptive embryonic/larval muscle system phenotype, suppressible by sns^XB3

(Artero et al., 2001)

Enhancer of

Statement

Reference

hbs⁴⁵⁹/hbs[+] is an enhancer of retina phenotype of Arf6^GD13822, Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF

(Johnson et al., 2011)

Suppressor of

Statement

Reference

hbs⁴⁵⁹ is a suppressor of primary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

hbs⁴⁵⁹ is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

hbs⁴⁵⁹ is a suppressor of pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

hbs⁴⁵⁹ is a suppressor of secondary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

hbs⁴⁵⁹ is a suppressor of tertiary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Other

Statement

Reference

dock⁰⁴⁷²³, hbs⁴⁵⁹ has embryonic myoblast phenotype

(Kaipa et al., 2013)

hbs⁴⁵⁹/hbs²⁵⁹³, sns^XB3/sns^D1 has muscle founder cell | embryonic stage phenotype

(Shelton et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

dock⁰⁴⁷²³ hbs⁴⁵⁹ double mutant embryos have severe defects in myoblast fusion.

(Kaipa et al., 2013)

Psn⁹/Psn¹⁴³ strongly enhances the thoracic bristle loss of hbs⁴⁵⁹/Df(2R)ED2423 mutants.

(Singh and Mlodzik, 2012)

The patterning defects in retinas co-expressing Arf51F^GD13822 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF is enhanced by heterozygosity for hbs⁴⁵⁹.

(Johnson et al., 2011)

sns^XB3, hbs²⁵⁹³ / sns^D1, hbs⁴⁵⁹ embryos have a relatively low number of eve-expressing DA1 founder cells - fewer than in sns^XB3 embryos.

The limited number of unfused myoblasts in hbs²⁵⁹³/hbs⁴⁵⁹ embryos increases when embryos are also heterozygous for sns^XB3 or sns^D1.

(Shelton et al., 2009)

Pupae heterozygous for hbs⁴⁵⁹ exhibit mild but consistent suppression of the Scer\GAL4^GMR.PF>cindr^{dsRNA.PC.PD.Scer\UAS} phenotype. The prevalence of cone and 1[o] cell defects is decreased and the number of 2[o] and 3[o] cells is reduced to slightly below normal.

(Johnson et al., 2008)

Both the gut and the somatic mesoderm phenotypers seen in hbs⁴⁵⁹/hbs²⁵⁹³ embryos are suppressed by the addition of sns^XB3.

(Artero et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

hbs^a69

(Tan et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

hbs⁴⁵⁹

(Tan et al., 2020, Kaipa et al., 2013, Singh and Mlodzik, 2012, Johnson et al., 2011, Sugie et al., 2010, Grillo-Hill and Wolff, 2009, Shelton et al., 2009, Johnson et al., 2008, Menon et al., 2005)

hibris⁴⁵⁹

(Bao and Cagan, 2005)

Name Synonyms

Secondary FlyBase IDs

References (11)

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