FlyBase Allele Report: Dmel\trbl[EP1119]

General Information

Symbol

Dmel\trbl^EP1119

Species

D. melanogaster

Name

FlyBase ID

FBal0117325

Feature type

allele

Associated gene

Dmel\trbl

Associated Insertion(s)

P{EP}trbl^EP1119

Carried in Construct

Also Known As

trbl¹¹¹⁹

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Mata et al., 2000)

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, Großhans and Wieschaus, 2000, Mata et al., 2000, Seher and Leptin, 2000)

Progenitor genotype

Associated Insertion(s)

P{EP}trbl^EP1119

Cytology

Description

P{EP} insertion in the 5' untranslated region.

(Großhans and Wieschaus, 2000)

P{EP} insertion in the 5' UTR of trbl. The transcriptional orientation of the P{EP} element is opposite to the direction of transcription of the trbl locus.

(Mata et al., 2000)

The P{EP} insertion is 186bp downstream of the presumed transcription start site of trbl.

(Seher and Leptin, 2000)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:20,401,299..20,401,299 [+]

(Liu et al., 2020, Liu et al., 2019, Otsuki and Brand, 2018, Nabel-Rosen et al., 2005, Gene Disruption Project members, 2001-, Großhans and Wieschaus, 2000, Mata et al., 2000, Seher and Leptin, 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | recessive

(Mata et al., 2000)

abnormal size | oogenesis

(Liu et al., 2019)

partially lethal (with trbl^D3)

(Masoner et al., 2013)

partially lethal (with trbl^D13)

(Masoner et al., 2013)

partially lethal | recessive

(Mata et al., 2000)

partially lethal - majority live

(Großhans and Wieschaus, 2000)

Phenotype Manifest In

cyst cell of ovary

(Mata et al., 2000)

cyst cell of ovary (with Df(3L)rdgC-co2)

(Mata et al., 2000)

cyst cell of ovary (with Df(3L)ri-79c)

(Mata et al., 2000)

embryo | gastrula stage

(Mata et al., 2000)

mesoderm anlage

(Mata et al., 2000)

mitotic domain 1

(Mata et al., 2000)

mitotic domain 2

(Mata et al., 2000)

mitotic domain 3

(Mata et al., 2000)

oocyte

(Mata et al., 2000)

oocyte (with Df(3L)rdgC-co2)

(Mata et al., 2000)

oocyte (with Df(3L)ri-79c)

(Mata et al., 2000)

oocyte nucleus

(Liu et al., 2019)

Detailed Description

Statement

Reference

The egg chambers in the ovaries of trbl^EP1119 females frequently display an enlarged oocyte nucleus.

(Liu et al., 2019)

Mutant embryos show a gastrulation phenotype.

(Großhans and Wieschaus, 2000)

45% of mutant embryos hatch but look abnormal and die during larval stages. 14% of homozygotes or hemizygotes survive to adulthood. 20% of egg chambers in surviving homozygous, trbl^EP1119/Df(3L)rdgC-co2 or trbl^EP1119/Df(3L)ri-79c females contain 8-cell cysts. Half of these cysts contain an oocyte, half do not. Mutant embryos derived from hemizygous mothers have a distorted pattern of mitosis. A very small percentage of embryos undergo an extra round of division before cellularisation. The majority of embryos show precocious mitosis occurring throughout a broad band in the ventral region (the mesoderm anlage) during late stage 5 or stage 6. Mitosis is either seen in this domain by itself, or in this domain plus several smaller patches in the head region. These smaller patches correspond to the normal mitotic domains 1-3. The embryos show severe defects in gastrulation, although some embryos appear to recover after being delayed or deformed when normal gastrulation should have taken place. The phenotype of premature ventral mitosis and the resulting gastrulation phenotype can be rescued by a wild-type paternal copy of trbl, indicating that these phenotypes are primarily due to lack of zygotic trbl expression.

(Mata et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

PpV^X9, trbl^EP1119 has abnormal size | oogenesis phenotype

(Liu et al., 2019)

PpV^X9, trbl^EP1119 has increased cell number | germline clone | oogenesis phenotype

(Liu et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

trbl^EP1119 has phenotype, suppressible by stg^unspecified

(Mata et al., 2000)

Suppressor of

Statement

Reference

trbl^EP1119/trbl^EP1119 is a suppressor of border follicle cell phenotype of slbo⁰¹³¹⁰

(Masoner et al., 2013)

Df(3L)ri-79c/trbl^EP1119 is a suppressor of border follicle cell phenotype of slbo^unspecified

(Rorth et al., 2000)

Other

Statement

Reference

PpV^X9, trbl^EP1119 has egg chamber phenotype

(Liu et al., 2019)

PpV^X9, trbl^EP1119 has nurse cell | oogenesis phenotype

(Liu et al., 2019)

PpV^X9, trbl^EP1119 has nucleus | oogenesis phenotype

(Liu et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

PpV^X9 germline clonal egg chambers in trbl^EP1119 females display multiple defects including disorganized egg chambers and egg chambers with an increased number of nurse cells.

(Liu et al., 2019)

trbl^EP1119/trbl^EP1119 partially suppresses the border cell migration defects seen in slbo⁰¹³¹⁰/slbo⁰¹³¹⁰ females.

(Masoner et al., 2013)

Embryos doubly mutant for trbl and stg do not show the trbl mutant phenotype.

(Mata et al., 2000)

The slbo^unspecified border cell phenotype is suppressed by trbl^EP1119/Df(3L)ri-79c.

(Rorth et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

CG33969^EY20583

(Masoner et al., 2013)

Rescued by

trbl^EP1119 is rescued by trbl^+tMa

(Mata et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58440

w¹¹¹⁸; P{EP}trbl^EP1119/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Order of allelic strength: trbl^D13 = trbl^D3 > trbl^EP3519 > trbl^EP1119.

(Masoner et al., 2013)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

EP(3)1119

(Großhans and Wieschaus, 2000)

trbl¹¹¹⁹

(LaFerriere and Zars, 2017, Rorth et al., 2000)

trbl^EP1119

(Liu et al., 2020, Otsuki and Brand, 2018, Masoner et al., 2013)

trbl^EP119

(Nabel-Rosen et al., 2005)

tribbles¹¹¹⁹

(Mata et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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