FlyBase Allele Report: Dmel\stumps[09904b]

General Information

Symbol

Dmel\stumps^09904b

Species

D. melanogaster

Name

FlyBase ID

FBal0095185

Feature type

allele

Associated gene

Dmel\stumps

Associated Insertion(s)

Carried in Construct

Also Known As

dof¹, dof^P1740

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Imam et al., 1999)

Mutagen

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Imam et al., 1999)

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

astrocyte-like glial cell | embryonic stage | somatic clone

(Stork et al., 2014)

dorsal air sac primordium

(Cabernard and Affolter, 2005)

dorsal vessel primordium

(Vincent et al., 1998)

egg

(Perrimon et al., 1996)

embryonic/larval dorsal vessel

(Imam et al., 1999)

embryonic/larval tracheal system

(Imam et al., 1999, Vincent et al., 1998)

embryonic midgut constriction 2

(Imam et al., 1999)

embryonic pericardial cell

(Dossenbach et al., 2001)

glial cell projection | embryonic stage | somatic clone

(Stork et al., 2014)

larval longitudinal connective

(Imam et al., 1999)

mesoderm

(Wilson et al., 2005, Imam et al., 1999, Imam et al., 1999, Vincent et al., 1998)

mesoderm | dorsal

(Imam et al., 1999)

presumptive embryonic/larval central nervous system

(Imam et al., 1999)

somatic mesoderm

(Vincent et al., 1998)

tracheal section

(Imam et al., 1999)

visceral mesoderm

(Vincent et al., 1998)

Detailed Description

Statement

Reference

Processes of single cell homozygous astrocyte clones are largely restricted to the surface of the neuropil and the size of the astrocytic domain is severely reduced compared to controls.

(Stork et al., 2014)

stumps^09904b homozygous clones in the dorsal air sac primordium grow normally but never contribute to the tip of the primordium.

(Cabernard and Affolter, 2005)

Pericardial cells fail to form in mutant embryos, due to defects in mesoderm migration.

(Dossenbach et al., 2001)

Tracheal cells fail to move out from the tracheal epithelial sacs and only rudimentary tracheal branches form. Mesodermal cells also fail in their dorsal migrations after gastrulation.

(Imam et al., 1999)

The peripheral nervous system appears normal, however there are defects in the central nervous system. These included increased separation, irregularities, and occasional breaks in the longitudinal connectives and fusions and irregularities in the transverse connectives. The initial steps in tracheal development are normal. However, during stages 11-14, when tracheal cells in the sac normally migrate toward bnl expressing cell clusters and form primary branches, tracheal cells in stumps^09904b mutants move very little. During stages 15-16, when the first terminal branches should be forming the tracheal sacs in mutants are still unbranched with only an occasional rudimentary branch. stumps^09904b also has a an effect on the mesoderm. Mesoderm cells appear to invaginate normally at the ventral midline but fail in their dorsal migrations. This phenotype manifests itself in older embryos as an absence of the heart and most other dorsal mesoderm derivatives. This phenotype decreases in severity from dorsal to ventral positions, implying that the mesodermal cells that fail to migrate can still undergo many aspects of normal ventral muscle differentiation. There is also a visceral muscle phenotype that includes the absence of the second gut constriction. Heterozygotes do not show a tracheal phenotype. The tracheal phenotype of hemizygous (stumps^09904b/Df(3R)ry506-85C or stumps^09904b/Df(3R)γ2) embryos is indistinguishable from stumps^09904b homozygotes.

(Imam et al., 1999)

Homozygous embryos have defects in the mesoderm and tracheal system. Determination of tracheal cell fate and cell division are normal, but the tracheal cells do not migrate and consequently the tracheal network fails to form. The invaginated mesodermal tube fails to flatten and spread properly in homozygous embryos. It does disperse into individual cells and the cells that contact the ectoderm eventually migrate dorsally. Later in development, no heart precursors are detected, the musculature is disrupted and less visceral mesoderm is produced than normal. Tracheal cell migration is rescued in stumps^09904b embryos expressing stumps^Scer\UAS.cVa under the control of Scer\GAL4^btl.PS; migration defects in these embryos are restricted to the visceral branch, probably due to the visceral mesoderm defects in these embryos. Mesodermal defects are rescued in stumps^09904b embryos expressing stumps^Scer\UAS.cVa under the control of Scer\GAL4^twi.PGa.

(Vincent et al., 1998)

Germline clones homozygous for part of the "l(3)09904" chromosome (which carries both stumps^09904b and l(3)09904c^09904c) made by homologous recombination using the FRT^3R-82B site at 82B produce abnormal eggs due to abnormal oogenesis: collapsed eggs.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

stumps^09904b has abnormal cell migration | embryonic stage phenotype, suppressible by btl::tor^4021.UAS/Scer\GAL4^btl.PS

(Vincent et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

btl^H82Δ3, stumps^09904b has ganglionic tracheal branch primordium phenotype, suppressible by btl::tor^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has tracheal dorsal trunk primordium phenotype, suppressible | partially by btl::Egfr^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has dorsal tracheal branch primordium phenotype, suppressible | partially by btl::Egfr^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has ganglionic tracheal branch primordium phenotype, suppressible | partially by btl::Egfr^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has tracheal lateral trunk primordium phenotype, suppressible | partially by btl::Egfr^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

stumps^09904b has embryonic pericardial cell phenotype, suppressible by Scer\GAL4^twi.PG/Egfr::htl^UAS.cDa

(Dossenbach et al., 2001)

stumps^09904b has embryonic pericardial cell phenotype, suppressible by htl::tor^UAS.cDa/Scer\GAL4^twi.PG

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has dorsal tracheal branch primordium phenotype, suppressible by btl::tor^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has tracheal lateral trunk primordium phenotype, suppressible by btl::tor^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

stumps^09904b has embryonic/larval tracheal system phenotype, suppressible by Scer\GAL4^twi.PG/bnl^UAS.cSa

(Imam et al., 1999)

stumps^09904b has embryonic/larval tracheal system phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^hs.PB

(Imam et al., 1999)

stumps^09904b has mesoderm phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^twi.PG

(Imam et al., 1999)

stumps^09904b has embryonic/larval tracheal system phenotype, suppressible by Ras85D^V12.UAS/Scer\GAL4^btl.PS

(Imam et al., 1999, Vincent et al., 1998)

stumps^09904b has embryonic/larval tracheal system phenotype, suppressible by Raf::tor^13D.hs.sev

(Vincent et al., 1998)

stumps^09904b has embryonic/larval tracheal system phenotype, suppressible by tor^13D.hs.sev

(Vincent et al., 1998)

NOT suppressed by

Statement

Reference

stumps^09904b has mesoderm phenotype, non-suppressible by Scer\GAL4^twi.PG/Cdc42^V12.UAS

(Wilson et al., 2005)

stumps^09904b has mesoderm phenotype, non-suppressible by Scer\GAL4^twi.PG/Rac1^V12.UAS

(Wilson et al., 2005)

stumps^09904b has mesoderm phenotype, non-suppressible by sqh^E20.E21

(Wilson et al., 2005)

btl^H82Δ3, stumps^09904b has presumptive embryonic/larval tracheal system phenotype, non-suppressible by btl^UAS.cDa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has presumptive embryonic/larval tracheal system phenotype, non-suppressible by btl::htl^BH.UAS/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

stumps^09904b has embryonic pericardial cell phenotype, non-suppressible by btl::htl^HB.UAS/Scer\GAL4^twi.PG

(Dossenbach et al., 2001)

stumps^09904b has embryonic pericardial cell phenotype, non-suppressible by Scer\GAL4^twi.PG/htl^UAS.cDa

(Dossenbach et al., 2001)

stumps^09904b has phenotype, non-suppressible by btl::tor^4021.UAS/Scer\GAL4^btl.PS

(Vincent et al., 1998)

Enhancer of

Statement

Reference

stumps^09904b/stumps[+] is an enhancer of dorsal group tracheal branch precursor phenotype of Rac1^J11, Rac2^Δ/Rac2[+]

(Chihara et al., 2003)

stumps^09904b/stumps[+] is an enhancer of tracheal branch primordium phenotype of Rac1^J11, Rac2^Δ/Rac2[+]

(Chihara et al., 2003)

stumps^09904b/stumps[+] is an enhancer of tracheal dorsal trunk primordium phenotype of Rac1^J11, Rac2^Δ/Rac2[+]

(Chihara et al., 2003)

stumps^09904b is an enhancer of embryonic/larval ganglionic tracheal branch phenotype of bnl⁰⁰⁸⁵⁷

(Imam et al., 1999)

stumps^09904b is an enhancer of embryonic/larval dorsal tracheal branch phenotype of bnl⁰⁰⁸⁵⁷

(Imam et al., 1999)

Other

Statement

Reference

Scer\GAL4^btl.PS, btl::Egfr^UAS.cDa, btl^H82Δ3, stumps^09904b has ganglionic tracheal branch primordium phenotype

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has ganglionic tracheal branch primordium phenotype

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has dorsal tracheal branch primordium phenotype

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has tracheal lateral trunk primordium phenotype

(Dossenbach et al., 2001)

Scer\GAL4^btl.PS, btl::Egfr^UAS.cDa, btl^H82Δ3, stumps^09904b has dorsal tracheal branch primordium phenotype

(Dossenbach et al., 2001)

Scer\GAL4^btl.PS, btl::Egfr^UAS.cDa, btl^H82Δ3, stumps^09904b has tracheal lateral trunk primordium phenotype

(Dossenbach et al., 2001)

Scer\GAL4^btl.PS, btl::Egfr^UAS.cDa, btl^H82Δ3, stumps^09904b has tracheal dorsal trunk primordium phenotype

(Dossenbach et al., 2001)

Scer\GAL4^btl.PS, btl::tor^UAS.cDa, btl^H82Δ3, stumps^09904b has ganglionic tracheal branch primordium phenotype

(Dossenbach et al., 2001)

Scer\GAL4^btl.PS, btl::tor^UAS.cDa, btl^H82Δ3, stumps^09904b has dorsal tracheal branch primordium phenotype

(Dossenbach et al., 2001)

Scer\GAL4^btl.PS, btl::tor^UAS.cDa, btl^H82Δ3, stumps^09904b has tracheal lateral trunk primordium phenotype

(Dossenbach et al., 2001)

btl^H82Δ3, stumps^09904b has presumptive embryonic/larval tracheal system phenotype

(Dossenbach et al., 2001)

bnl⁰⁰⁸⁵⁷, stumps^09904b has embryonic/larval tracheal dorsal trunk phenotype

(Imam et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Mesodermal cells in stumps^09904b stg⁴ double mutant embryos are able to disperse from the site of mesoderm invagination.

(Wilson et al., 2005)

Expression of btl^Scer\UAS.cDa or btl::htl^BH.Scer\UAS under the control of Scer\GAL4^btl.PS does not rescue any aspect of tracheal development in btl^H82Δ3 stumps^09904b double mutant embryos. Expression of btl::tor^Scer\UAS.cDa under the control of Scer\GAL4^btl.PS significantly rescues tracheal development in btl^H82Δ3 stumps^09904b double mutant embryos. Ganglionic branches are rescued almost completely, dorsal branches develop in more than 50% of segments and dorsal trunk fusion occurs efficiently. The lateral trunk does not fuse in these embryos and ectopic terminal cell formation is seen. Expression of btl::Egfr^Scer\UAS.cDa under the control of Scer\GAL4^btl.PS significantly rescues tracheal development in btl^H82Δ3 stumps^09904b double mutant embryos. The dorsal trunk is disrupted in some embryos, some dorsal branches are missing or misguided and the lateral trunk fails to fuse. Some of the ganglionic branches fail to form and do not migrate in the proper direction. Expression of htl^Scer\UAS.cDa or btl::htl^HB.Scer\UAS under the control of Scer\GAL4^twi.PG does not rescue pericardial cell development in stumps^09904b embryos. Expression of htl::tor^Scer\UAS.cDa or Egfr::htl^Scer\UAS.cDa under the control of Scer\GAL4^twi.PG efficiently rescues pericardial cell development in stumps^09904b embryos.

(Dossenbach et al., 2001)

stumps^09904b, bnl⁰⁰⁸⁵⁷ double heterozygotes show a dramatic increase in tracheal outgrowth defects, with nearly four times as many stalled ganglionic branches as the bnl⁰⁰⁸⁵⁷ heterozygote alone. The double heterozygotes also displayed increased dorsal branch outgrowth defects compared to bnl⁰⁰⁸⁵⁷ heterozygotes as well as rare dorsal trunk outgrowth defects never seen in bnl⁰⁰⁸⁵⁷ heterozygotes. When expression of bnl^Scer\UAS.cSa is driven in the mesoderm of a stumps^09904b mutant by Scer\GAL4^twi.PG, the portions of the tracheal sacs near the bnl^Scer\UAS.cSa expressing cells significant cytoplasmic outgrowth and ramification of terminal branches. Some erratic of primary branches also occurs. These features are not normally seen in stumps^09904b mutants. When expression of Ras85D^V12.Scer\UAS is driven in the trachea of mutants by Scer\GAL4^btl.PS or Scer\GAL4^hs.PB, tracheal cells show erratic outgrowth of primary branches. These features are not normally seen in stumps^09904b mutants. stumps^09904b/btl^LG18 double heterozygotes did not show any tracheal defects. When expression of Ras85D^V12.Scer\UAS is driven by Scer\GAL4^twi.PG in stumps^09904b embryos, some random mesodermal migrations are restored.

(Imam et al., 1999)

Expression of Ras85D^V12.Scer\UAS under the control of Scer\GAL4^btl.PS in stumps^09904b mutant embryos partially rescues tracheal morphogenesis. Expression of phl::tor^13D.hs.sev in stumps^09904b mutant embryos partially rescues tracheal morphogenesis. Expression of btl::tor^{4021.Scer\UAS} under the control of Scer\GAL4^btl.PS in stumps^09904b mutant embryos does not rescue tracheal migration defects. Expression of tor^13D.hs.sev in stumps^09904b mutant embryos induces some tracheal branching.

(Vincent et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

stumps^S083710a

(Vincent et al., 1998)

Rescued by

stumps^09904b is rescued by Scer\GAL4^twi.PG/stumps^UAS.cVa

(Dossenbach et al., 2001)

Partially rescued by

stumps^09904b is partially rescued by stumps^UAS.cVa/Scer\GAL4^btl.PS

(Dossenbach et al., 2001)

stumps^09904b is partially rescued by stumps^UAS.cVa/Scer\GAL4^btl.PS

(Vincent et al., 1998)

stumps^09904b is partially rescued by Scer\GAL4^twi.PGa/stumps^UAS.cVa

(Vincent et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11740

P{PZ}09904a, stumps^09904b l(3)09904c^09904c, ry⁵⁰⁶/MKRS

Notes on Origin

Discoverer

Induced with: P{PZ}09904a.

(Vincent et al., 1998)

Arose on: the "l(3)09904" chromosome. stumps^09904b was identified on the "l(3)09904" chromosome that carries the P{PZ}09904a insertion at 82F8--82F9 and also carries an independent lethal mutation (l(3)09904c^09904c) as well as the stumps^09904b mutation.

(Imam et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

P1740

(Dossenbach et al., 2001)

dof¹

(Schäfer et al., 2014, Stork et al., 2014, Clark et al., 2011, Wilson et al., 2005, Peri et al., 2002, Vincent et al., 1998)

dof^P1740

(Cabernard and Affolter, 2005, Chihara et al., 2003)

hbr¹

l(3)09904

(Perrimon et al., 1996)

l(3)9904

(Imam et al., 1999)

stumps⁰⁹⁹⁰⁴

stumps^09904b

(Cook et al., 2009.11.25)

stumps¹

(Imam et al., 1999)

stumps^hbr-1

Name Synonyms

Secondary FlyBase IDs

FBal0094529
FBal0098640

References (13)

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