FlyBase Allele Report: Dmel\bsk[UAS.cBa]

General Information

Symbol

Dmel\bsk^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Boutros

FlyBase ID

FBal0093136

Feature type

allele

Associated gene

Dmel\bsk

Associated Insertion(s)

Carried in Construct

P{UAS-bsk.B}

Also Known As

UAS-bsk, UAS-JNK, UAS-basket, UASBsk, UAS-bsk^B

Key Links

Transgenic product class

cDNA

(Boutros et al., 1998)

wild_type

(Boutros et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Boutros et al., 1998)

wild_type

(Boutros et al., 1998)

Mutagen

in vitro construct

(Boutros et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-bsk.B}

Cytology

Description

Expression of bsk cDNA is governed by UASt regulatory sequences.

(Boutros et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

bsk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^A30P.UAS

(Liu et al., 2022)

exacerbates human immunodeficiency virus infectious disease

modeled by HIV-1\nef^UAS.cLa

(Lee et al., 2005)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal planar polarity, with Scer\GAL4^hs.2sev

(Weber et al., 2000)

chemical resistant, with Scer\GAL4^arm.PS

(Wang et al., 2003)

increased cell death | third instar larval stage, with Scer\GAL4^en.PU

(Ko et al., 2016)

increased cell death | third instar larval stage, with Scer\GAL4^ptc.PU

(Ko et al., 2016)

visible, with Scer\GAL4^Bx-MS1096

(Hwang et al., 2010)

Phenotype Manifest In

adult optic lobe glial cell | cell non-autonomous, with Scer\GAL4^nSyb.PS

(Liu et al., 2017)

embryonic/larval fat body | somatic clone, with Scer\GAL4^Act.PU

(Chang and Neufeld, 2009)

eye, with Scer\GAL4^ey.PH

(Singh et al., 2006)

eye, with Scer\GAL4^hs.2sev

(Weber et al., 2000)

lipid droplet | adult stage | cell non-autonomous, with Scer\GAL4^nSyb.PS

(Liu et al., 2017)

ommatidium, with Scer\GAL4^hs.2sev

(Weber et al., 2000)

peripodial epithelium, with Scer\GAL4^{Ubx-lac1-Gal4}

(Tripura et al., 2011)

ventral furrow, with Scer\GAL4^twi.PG

(Mathew et al., 2011)

wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

(Ko et al., 2016)

wing disc posterior compartment | third instar larval stage, with Scer\GAL4^en.PU

(Ko et al., 2016)

wing vein | ectopic, with Scer\GAL4^Bx-MS1096

(Hwang et al., 2010)

Detailed Description

Statement

Reference

The expression of bsk^Scer\UAS.cBa under the control of either Scer\GAL4^ptc.PU and Scer\GAL4^en.PU can induce apoptosis (i.e. Casp3 expression) but is unable to cause significant cell extrusion, as compared to controls.

(Ko et al., 2016)

Neuronal expression of bsk^Scer\UAS.cBa (under the control of Scer\GAL4^elav.PU) leads to glial cell specific lipid droplet accumulation. Neuronal expression of SREBP^Scer\UAS.cKa alone does not cause neurodegeneration upon aging and lipid droplets are retained in aged animals.

Glial expression of bsk^Scer\UAS.cBa (under the control of Scer\GAL4^54C) does not lead to lipid droplet accumulation.

(Liu et al., 2015)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG results in delayed or defective ventral furrow formation in a proportion of embryos.

(Mathew et al., 2011)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^{Ubx-lac1-Gal4} results in transformation of posterior peripodial membrane cells of the wing disc from a hexagonal shape into an elongated shape.

(Tripura et al., 2011)

Over-expression of bsk^Scer\UAS.cBa driven in the wing by Scer\GAL4^Bx-MS1096 results in ectopic vein formation in the distal portion of the 4th and 5th longitudinal veins.

(Hwang et al., 2010)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^Act induces autophagy in clones in the larval fat body.

(Chang and Neufeld, 2009)

Expression of bsk^Scer\UAS.cBa in the wing, under the control of Scer\GAL4^Bx-MS1096 results in mild or undetectable phenotypic changes.

(Lee et al., 2006)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH results in eyes that are smaller than wild type.

(Singh et al., 2006)

Overexpression of bsk^Scer\UAS.cBa, under the regulation of Scer\GAL4^ap-md544 has no effect on thorax closure.

(Cha et al., 2005)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF does not affect eye development.

(Cha et al., 2003)

Flies expressing bsk^Scer\UAS.cBa, under the control of Scer\GAL4^arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies.

(Wang et al., 2003)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^elav-C155 has no effect on EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction.

(Sanyal et al., 2002)

When expression is driven by Scer\GAL4^hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form.

(Weber et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, bsk^UAS.cBa has visible phenotype, suppressible by park^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096

(Hwang et al., 2010)

Enhancer of

Statement

Reference

bsk^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

bsk^UAS.cBa, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Daxx^EY09290, Scer\GAL4^Bx-MS1096

(Hwang et al., 2013)

bsk^UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, by^UAS.cLa

(Lee et al., 2003)

Suppressor of

Statement

Reference

bsk^UAS.cBa/Scer\GAL4^Tub.PU is a suppressor of short lived | recessive phenotype of CYLD^mr4

(Xue et al., 2007)

bsk^UAS.cBa/Scer\GAL4^Tub.PU is a suppressor of abnormal oxidative stress response | recessive phenotype of CYLD^mr4

(Xue et al., 2007)

Scer\GAL4^ptc-559.1/bsk^UAS.cBa is a suppressor of lethal | embryonic stage phenotype of cno²

(Boettner et al., 2003)

bsk^UAS.cBa is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^elav-C155, puc^UAS.cMa

(Sanyal et al., 2002)

bsk^UAS.cBa is a suppressor of abnormal neurophysiology phenotype of Scer\GAL4^elav-C155, puc^UAS.cMa

(Sanyal et al., 2002)

NOT Suppressor of

Statement

Reference

bsk^UAS.cBa, Scer\GAL4^ptc-559.1 is a non-suppressor of lethal | embryonic stage phenotype of Rap1^{N17.UAS.Tag:MYC}, Scer\GAL4^ptc-559.1

(Boettner et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

bsk^UAS.cBa has eye phenotype, enhanceable by L²/L[+]

(Singh et al., 2006)

Suppressed by

Statement

Reference

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Sln^GD1940/Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Sln^GD1940/Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Sln^KK104306/Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Sln^KK104306/Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Scer\GAL4^nSyb.PS/out^GD3448

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Scer\GAL4^nSyb.PS/out^GD3448

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Scer\GAL4^nSyb.PS/out^KK104187

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Scer\GAL4^nSyb.PS/out^KK104187

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Ldh^HMS00039, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Ldh^HMS00039, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Pdha1^GD12103, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Pdha1^GD12103, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has adult optic lobe glial cell | cell non-autonomous phenotype, suppressible by Pdha1^KK101856, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS, bsk^UAS.cBa has lipid droplet | cell non-autonomous | adult stage phenotype, suppressible by Pdha1^KK101856, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^Bx-MS1096, bsk^UAS.cBa has wing vein | ectopic phenotype, suppressible by park^UAS.Tag:MYC/Scer\GAL4^Bx-MS1096

(Hwang et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act.PU, bsk^UAS.cBa has embryonic/larval fat body | somatic clone phenotype, non-suppressible by Atg13^Δ81/Atg13^Δ81

(Chang and Neufeld, 2009)

Enhancer of

Statement

Reference

bsk^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Ketosugbo et al., 2017)

bsk^UAS.cBa, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Daxx^EY09290, Scer\GAL4^Bx-MS1096

(Hwang et al., 2013)

bsk^UAS.cBa, Scer\GAL4^Bx-MS1096 is an enhancer of wing blade phenotype of Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

bsk^UAS.cBa, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Lkb1^UAS.cLa, Scer\GAL4^Bx-MS1096

(Lee et al., 2006)

bsk^UAS.cBa/Scer\GAL4^ey.PH is an enhancer of eye disc | ventral phenotype of L²

(Singh et al., 2006)

bsk^UAS.cBa/Scer\GAL4^ey.PH is an enhancer of eye | ventral phenotype of L²

(Singh et al., 2006)

bsk^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, Traf4^EP578

(Cha et al., 2003)

bsk^UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, by^UAS.cLa

(Lee et al., 2003)

NOT Enhancer of

Statement

Reference

bsk^UAS.cBa/Scer\GAL4^c415 is a non-enhancer of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

bsk^UAS.cBa/Scer\GAL4^dpp.PH is a non-enhancer of wing phenotype of Scer\GAL4^dpp.PH, eya^UAS.cHa

(Hsiao et al., 2001)

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/bsk^UAS.cBa is a suppressor of adult thorax phenotype of Scer\GAL4^ap-md544, park^UAS.Tag:MYC

(Cha et al., 2005)

Scer\GAL4^ap-md544/bsk^UAS.cBa is a suppressor of mesothoracic cleft | ectopic phenotype of Scer\GAL4^ap-md544, park^UAS.Tag:MYC

(Cha et al., 2005)

Scer\GAL4^ptc-559.1/bsk^UAS.cBa is a suppressor of embryo | dorsal closure stage phenotype of cno²

(Boettner et al., 2003)

bsk^UAS.cBa is a suppressor of bouton phenotype of Scer\GAL4^elav-C155, puc^UAS.cMa

(Sanyal et al., 2002)

bsk^UAS.cBa is a suppressor of neuromuscular junction | larval stage phenotype of Scer\GAL4^elav-C155, puc^UAS.cMa

(Sanyal et al., 2002)

bsk^UAS.cBa/Scer\GAL4^VP16.arm is a suppressor of embryonic epidermis | ventral phenotype of arm³

(McEwen et al., 2000)

NOT Suppressor of

Statement

Reference

bsk^UAS.cBa, Scer\GAL4^ptc-559.1 is a non-suppressor of embryo | dorsal closure stage phenotype of Rap1^{N17.UAS.Tag:MYC}, Scer\GAL4^ptc-559.1

(Boettner et al., 2003)

bsk^UAS.cBa/Scer\GAL4^c415 is a non-suppressor of dorsal appendage phenotype of bwk¹⁵¹, cic⁰⁸⁴⁸²

(Tran and Berg, 2003)

bsk^UAS.cBa/Scer\GAL4^dpp.blk1 is a non-suppressor of eye | ectopic phenotype of Scer\GAL4^dpp.blk1, eya^UAS.cHa

(Hsiao et al., 2001)

bsk^UAS.cBa/Scer\GAL4^dpp.PH is a non-suppressor of wing phenotype of Scer\GAL4^dpp.PH, eya^UAS.cHa

(Hsiao et al., 2001)

bsk^UAS.cBa/Scer\GAL4^VP16.arm is a non-suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The accumulation of lipid droplets in the retinal glia of adult flies expressing bsk^Scer\UAS.cBa under the control of Scer\GAL4^nSyb.PS is significantly suppressed by co-expression of any of the following: Sln^GD1940, Sln^KK104306, out^GD3448, out^KK104187, Ldh^HMS00039, Pdha^GD12103 or Pdha^KK101856.

(Liu et al., 2017)

The reduced wing phenotype caused by expression of DLP^EY09290 under the control of Scer\GAL4^Bx-MS1096 is strongly exacerbated by co-expression of bsk^Scer\UAS.cBa.

(Hwang et al., 2013)

Co-expression of park^{Scer\UAS.T:Hsap\MYC} strongly suppresses the ectopic wing veins seen with expression of bsk^Scer\UAS.cBa driven by Scer\GAL4^Bx-MS1096.

(Hwang et al., 2010)

The autophagy that is induced by expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^Act in clones in the larval fat body still occurs in an Atg13^Δ81 homozygous background.

(Chang and Neufeld, 2009)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU rescues both the shortened lifespan increased sensitivity to paraquat which is seen in CYLD^mr4 mutant adults (animals homozygous for Df(2L)CYLD^mr4 and carrying P{LX107} to rescue the function of CG13138 and nmd).

(Xue et al., 2007)

Simultaneous expression of bsk^Scer\UAS.cBa with lkb1^Scer\UAS.cLa (both under the control of Scer\GAL4^Bx-MS1096 strongly enhances the lkb1^Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

(Lee et al., 2006)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ey.PH enhances the loss-of-ventral-eye phenotype to near complete loss in 39% of L²/+ flies.

(Singh et al., 2006)

The thoracic cleft formation observed in park^{Scer\UAS.T:Hsap\MYC} overexpression mutants (Scer\GAL4^ap-md544) is suppressed by co-expression of bsk^Scer\UAS.cBa.

(Cha et al., 2005)

Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ptc-559.1 results in a significant but partial rescue of the dorsal closure defects seen in cno² embryos. Co-expression of bsk^Scer\UAS.cBa does not rescue the dorsal closure defects seen in embryos expressing R^{N17.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1.

(Boettner et al., 2003)

Coexpression of bsk^Scer\UAS.cBa with Traf1^EP578, under the control of Scer\GAL4^GMR.PF, leads to an increase in the disturbance of the ommatidial array in the compound eye in comparison to the eye phenotype resulting from one copy overexpression of Traf1^EP578.

Coexpression of Traf2^EP1516 with either bsk^Scer\UAS.cBa, under the control of Scer\GAL4^GMR.PF, does not affect the development of eyes.

(Cha et al., 2003)

The mutant dorsal appendage phenotype seen in bwk¹⁵¹/bwk⁰⁸⁴⁸² eggs is not significantly modified by expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^c415.

(Tran and Berg, 2003)

The proportion of eya^Scer\UAS.cHa; Scer\GAL4^dpp.PH flies with blistered wings (76%) is relatively unaffected by the presence of bsk^Scer\UAS.cBa (82%), as is the proprotion with only mild wing defects 24% compared to 18%. (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

(Hsiao et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Tab2-201Y/bsk^UAS.cBa rescues bsk^LL02244

(Bornstein et al., 2015)

Comments

Expressing bsk^Scer\UAS.cBa in bsk^LL02244/bsk^LL02244 clones (with Scer\GAL4^Tab2-201Y) rescues axon-pruning defects.

(Bornstein et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9310

w^*; P{UAS-bsk.B}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

bsk^Scer\UAS.cBa

bsk^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Boutros

(Boutros et al., 1998)

Secondary FlyBase IDs

References (34)

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