FlyBase Allele Report: Dmel\out[GD3448]

General Information

Symbol

Dmel\out^GD3448

Species

D. melanogaster

Name

FlyBase ID

FBal0205715

Feature type

allele

Associated gene

Dmel\out

Associated Insertion(s)

Carried in Construct

P{GD3448}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3448}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3448

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^repo.PU

(Volkenhoff et al., 2015)

visible, with Scer\GAL4^nSyb.PS

(Volkenhoff et al., 2015)

Phenotype Manifest In

wing, with Scer\GAL4^nSyb.PS

(Volkenhoff et al., 2015)

Detailed Description

Statement

Reference

Flies with expression of out^GD3448 driven by Scer\GAL4^nSyb.PS have folded wings.

(Volkenhoff et al., 2015)

Adults expressing out^GD3448 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

out^GD3448, Scer\GAL4^repo is a non-enhancer of abnormal wound healing | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Scer\GAL4^ppk.PU/out^GD3448 is a non-enhancer of abnormal wound healing | larval stage phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

Suppressor of

Statement

Reference

out^GD3448/Scer\GAL4^ey.PU is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of Marf^B

(Liu et al., 2017)

out^GD3448/Scer\GAL4^ey.PU is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of sicily^E

(Liu et al., 2017)

NOT Suppressor of

Statement

Reference

out^GD3448, Scer\GAL4^repo is a non-suppressor of abnormal wound healing | larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Scer\GAL4^ppk.PU/out^GD3448 is a non-suppressor of abnormal wound healing | larval stage phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

out^GD3448, Scer\GAL4^repo is a non-enhancer of larval multidendritic class IV neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Scer\GAL4^ppk.PU/out^GD3448 is a non-enhancer of larval multidendritic class IV neuron phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

Suppressor of

Statement

Reference

out^GD3448/Scer\GAL4^ey.PU is a suppressor of lipid droplet | somatic clone | adult stage phenotype of sicily^E

(Liu et al., 2017)

out^GD3448/Scer\GAL4^ey.PU is a suppressor of photoreceptor | somatic clone | adult stage | progressive phenotype of sicily^E

(Liu et al., 2017)

out^GD3448/Scer\GAL4^ey.PU is a suppressor of adult optic lobe glial cell | somatic clone phenotype of Marf^B

(Liu et al., 2017)

out^GD3448/Scer\GAL4^ey.PU is a suppressor of lipid droplet | somatic clone | adult stage phenotype of Marf^B

(Liu et al., 2017)

out^GD3448/Scer\GAL4^ey.PU is a suppressor of photoreceptor | somatic clone | adult stage | progressive phenotype of Marf^B

(Liu et al., 2017)

out^GD3448, Scer\GAL4^nSyb.PS is a suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of SREBP^UAS.cKa, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

out^GD3448, Scer\GAL4^nSyb.PS is a suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of SREBP^UAS.cKa, Scer\GAL4^nSyb.PS

(Liu et al., 2017)

Scer\GAL4^nSyb.PS/out^GD3448 is a suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of Scer\GAL4^nSyb.PS, bsk^UAS.cBa

(Liu et al., 2017)

Scer\GAL4^nSyb.PS/out^GD3448 is a suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of Scer\GAL4^nSyb.PS, bsk^UAS.cBa

(Liu et al., 2017)

Scer\GAL4^elav.PLu/out^GD3448 is a suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Scer\GAL4^elav.PLu/out^GD3448 is a suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

out^GD3448/Scer\GAL4^ey.PU is a suppressor of adult optic lobe glial cell | somatic clone phenotype of sicily^E

(Liu et al., 2017)

NOT Suppressor of

Statement

Reference

out^GD3448, Scer\GAL4^repo is a non-suppressor of larval multidendritic class IV neuron phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo, btl::Egfr^λ.UAS

(Li et al., 2020)

Scer\GAL4^ppk.PU/out^GD3448 is a non-suppressor of larval multidendritic class IV neuron phenotype of Myc^QUAS.cPa, Ncra\QF^repo

(Li et al., 2020)

Scer\GAL4^54C/out^GD3448 is a non-suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Scer\GAL4^54C/out^GD3448 is a non-suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The accumulation of lipid droplets in glial cells in the vicinity of adult photoreceptor neurons expressing ND-42^ninaE.GD6220 is not suppressed by Scer\GAL4^54C-driven (glial) expression of out^GD3448 while expression under the control of the neuronal Scer\GAL4^elav.PLu driver efficiently rescues the phenotype.

Expression of out^GD3448 in the ey>flip whole-eye clones mutant for either sicily^E or Marf^B suppresses the lipid droplet accumulation in retinal glial cells in adult flies and also improves the photoreceptor degeneration phenotype.

The accumulation of lipid droplets in the retinal glia of adult flies expressing either SREBP^UAS.cKa or bsk^Scer\UAS.cBa under the control of Scer\GAL4^nSyb.PS is significantly suppressed by co-expression of out^GD3448.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v51157

w¹¹¹⁸; P{GD3448}v51157

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG8062^GD3448

out^GD3448

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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