FlyBase Allele Report: Dmel\eya[UAS.cHa]

General Information

Symbol

Dmel\eya^UAS.cHa

Species

D. melanogaster

Name

UAS construct of Hsiao

FlyBase ID

FBal0150506

Feature type

allele

Associated gene

Dmel\eya

Associated Insertion(s)

Carried in Construct

P{UAS-eya.H}

Also Known As

UAS-eya^WT, UAS-eya

Key Links

Transgenic product class

wild_type

(Hsiao et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Hsiao et al., 2001)

Mutagen

in vitro construct

(Hsiao et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-eya.H}

Cytology

Description

Expression of the eya coding region is under the control of UASt sequences.

(Hsiao et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

eya

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

arista, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

eye, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

eye | ectopic, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

head, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

leg, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

leg | ectopic, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

macrochaeta | ectopic, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

metathoracic leg, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

scutellar bristle | increased number, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

wing, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

wing, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

wing hinge, with Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

wing vein L3, with Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

Detailed Description

Statement

Reference

The average penetrance of ectopic eye induction in eya^Scer\UAS.cHa; Scer\GAL4^dpp.blk1 flies is 49% (over 8 independent P{UAS-eya.H} insertions). Ectopic eyes are typically seen on the head, either close to the eyes and near the base of the antennae. Patches of red pigement are also frequently seen in the wing hinges and on the underside of metathoracic legs, and ocassionally elsewhere on wings legs and antennae. The penetrance of ectopic eye induction in eya^Scer\UAS.cHa; Scer\GAL4^dpp.blk1 flies is reduced from 98% (n=298) to 50% (n=313) by heterozygosity for eya^G130 (data from a single, 'strong' P{UAS-eya.H} insertion). eya^Scer\UAS.cHa; Scer\GAL4^dpp.PH flies have highly variable wing defects, including ectopic bristles on L3 and in more extreme cases, blistering, and defects in overall shape: Proportion with blistered wings = 40%; proportion with only mild wing defects = 60%. These flies also have increased numbers of scutellar machrochaete (average= 6.0, compared to 4 in wild-type), and problems with thoracic closure. They also have a low penetrance of rough eyes, and arista to leg transformations. Note: all of the above data is an average result from 8 independent P{UAS-eya.H} insertions. Heterozygosity for eya^G130 leads to a decrease in the proportion of eya^Scer\UAS.cHa; Scer\GAL4^dpp.PH flies with blistered wings (76% to 3%) and an increase in the proportion with only milder wing defects (24% to 97%) (data from a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

(Hsiao et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cHa has eye | ectopic phenotype, enhanceable by rl^Sem.cUa.UAS/Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, enhanceable by rl^Sem.cUa.UAS/Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

NOT Enhanced by

Statement

Reference

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, non-enhanceable by bsk^UAS.cBa/Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, non-enhanceable by bsk¹

(Hsiao et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cHa has eye | ectopic phenotype, suppressible by Egfr^unspecified

(Hsiao et al., 2001)

Scer\GAL4^dpp.blk1, eya^UAS.cHa has eye | ectopic phenotype, suppressible by rl^S-135

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, suppressible by Egfr^unspecified

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, suppressible by rl^S-135

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, suppressible by dac³

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, suppressible by so³

(Hsiao et al., 2001)

NOT suppressed by

Statement

Reference

Scer\GAL4^dpp.blk1, eya^UAS.cHa has eye | ectopic phenotype, non-suppressible by bsk¹

(Hsiao et al., 2001)

Scer\GAL4^dpp.blk1, eya^UAS.cHa has eye | ectopic phenotype, non-suppressible by bsk^UAS.cBa/Scer\GAL4^dpp.blk1

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, non-suppressible by bsk¹

(Hsiao et al., 2001)

Scer\GAL4^dpp.PH, eya^UAS.cHa has wing phenotype, non-suppressible by bsk^UAS.cBa/Scer\GAL4^dpp.PH

(Hsiao et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance of ectopic eye induction in eya^UAS.cHa; Scer\GAL4^dpp.blk1 flies is reduced from 98% (n=298) to 50% (n=313) by heterozygosity for rl^S-135 and to 58% (n=313), by heterozygosity for Egfr^unspecified (data from a single, 'strong' P{UAS-eya.H} insertion). No suppression of penetrance was seen due to heterozygosity for bsk¹ or the presence of rl^Sem.cUa.UAS, or bsk^UAS.cBa. Ectopic eyes in eya^UAS.cHa; Scer\GAL4^dpp.blk1; rl^Sem.cUa.UAS are generally larger, and have more ommatidial definition than those in eya^UAS.cHa; Scer\GAL4^dpp.blk1 flies. The proportion of eya^UAS.cHa; Scer\GAL4^dpp.PH flies with blistered wings (76%) is decreased by heterozygosity for dac³ (to 4%), so³ (to 17%), rl^S-135 (to 10%), or Egfr^unspecified (to 14%), increased by heterozygosity for rl^Sem.cUa.UAS (to 93%), and relatively unaffected by heterozygosity for bsk¹ (69%), or the presence of bsk^UAS.cBa (82%). Conversely, the proportion of eya^UAS.cHa; Scer\GAL4^dpp.PH flies with only mild wing defects (24%) is increased by heterozygosity for dac³ (to 96%), so³ (to 83%), rl^S-135 (to 90%), or Egfr^unspecified (to 86%) decreased by heterozygosity for rl^Sem.cUa.UAS (to 7%), and relatively unaffected by heterozygosity for bsk¹ (31%), or the presence of bsk^UAS.cBa (18%). (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

(Hsiao et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^ey.PB/eya^UAS.cHa partially rescues eya²

(Rayapureddi et al., 2003)

Comments

eya^Scer\UAS.cHa; Scer\GAL4^ey.PB partially rescues the loss of eye phenotype of eya² mutant flies.

(Rayapureddi et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

eya^Scer\UAS.cHa

eya^UAS.cHa

Name Synonyms

UAS construct of Hsiao

(Hsiao et al., 2001)

Secondary FlyBase IDs

References (5)

Report Sections

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