FlyBase Allele Report: Dmel\CadN[UAS.cIa]

General Information

Symbol

Dmel\CadN^UAS.cIa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Iwai

FlyBase ID

FBal0066231

Feature type

allele

Associated gene

Dmel\CadN

Associated Insertion(s)

Carried in Construct

P{UAS-CadN.I}

Also Known As

UAS-N-cad, UAS-Ncad, UAS-DN-cadherin, UAS-CadN

Key Links

Transgenic product class

cDNA

(Iwai et al., 1997)

wild_type

(Iwai et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Iwai et al., 1997)

wild_type

(Iwai et al., 1997)

Mutagen

in vitro construct

(Iwai et al., 1997)

Progenitor genotype

Carried in construct

P{UAS-CadN.I}

Cytology

Description

Encodes a CadN isoform which contains the alternative exons 7a, 13a and 18a.

(Zhu and Luo, 2004)

UASt regulatory sequences drive expression of a 9.3kb CadN cDNA.

(Iwai et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

CadN

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon terminus | larval stage, with Scer\GAL4^bsh.M.PT

(Trush et al., 2019)

axon terminus | larval stage, with Scer\GAL4^sens.F2

(Trush et al., 2019)

axon terminus | larval stage | somatic clone, with Scer\GAL4^bsh.M.PT

(Trush et al., 2019)

cone cell | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PI

(Hayashi and Carthew, 2004)

medulla | larval stage, with Scer\GAL4^bsh.M.PT

(Trush et al., 2019)

medulla | larval stage, with Scer\GAL4^sens.F2

(Trush et al., 2019)

medulla | larval stage | somatic clone, with Scer\GAL4^bsh.M.PT

(Trush et al., 2019)

medullary intrinsic neuron Mi1 | larval stage, with Scer\GAL4^bsh.M.PT

(Trush et al., 2019)

medullary intrinsic neuron Mi1 | larval stage | somatic clone, with Scer\GAL4^bsh.M.PT

(Trush et al., 2019)

ommatidial precursor cluster, with Scer\GAL4^hs.2sev

(Mirkovic and Mlodzik, 2006)

ommatidium | somatic clone, with Scer\GAL4^hs.2sev

(Mirkovic and Mlodzik, 2006)

photoreceptor cell R7 | larval stage, with Scer\GAL4^sens.F2

(Trush et al., 2019)

photoreceptor cell R8 | larval stage, with Scer\GAL4^sens.F2

(Trush et al., 2019)

primary pigment cell | somatic clone, with Scer\GAL4^Act5C.PI

(Hayashi and Carthew, 2004)

Detailed Description

Statement

Reference

The expression of CadN^UAS.cIa under the control of Scer\GAL4^bsh.M.PT results in the Mi1 neuron terminals in the larval medulla redirecting to the column center and overlapping with R8 photoreceptor terminals, instead of projecting to the grid-like domain outside the columns; the same was observed upon clonal expression of in R7 photoreceptors.

The expression of CadN^UAS.cIa under the control of Scer\GAL4^sens.F2 results in the R8 photoreceptor terminals in the larval medulla expanding and redirecting to the center of the column and the shrinkage of the central hole area; R7 photoreceptor terminals are also expanded toward the column periphery, overlapping with R8 photoreceptor terminals.

The expression of CadN^UAS.cIa under the control of Scer\GAL4^sev.PM181 does not lead to significant changes in R7 photoreceptor axon projections in the larval medulla.

(Trush et al., 2019)

Expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^GH146 does not suppress the dendrite targeting defects seen in homozygous meigo¹ projection neuron clones.

(Sekine et al., 2013)

Clones in the eye that overexpress CadN^Scer\UAS.cIa under the control of Scer\GAL4^hs.2sev show a rotation defects with ommatidia rotating to a random degree. Random rotation angles are also observed in Scer\GAL4^hs.2sev>CadN^Scer\UAS.cIa third instar eye discs.

(Mirkovic and Mlodzik, 2006)

Expression of CadN^Scer\UAS.cIa in CadN⁴⁰⁵ mutant R7 neurons, under the control of Scer\GAL4^tub, increases the percentage of mutant R7 clones with correct target layer selection from 0% to 95%. Expression of CadN^Scer\UAS.cIa, under the control of Scer\GAL4^tub, increases the proportion of wild-type R1-R6 targeting in CadN⁴⁰⁵ mutant clones.

(Nern et al., 2005)

Clones of CadN^Scer\UAS.cIa; Scer\GAL4^Act5C.PI cells in the pupal retina: When both primary pigment cells in a ommatidium are CadN^Scer\UAS.cIa; Scer\GAL4^Act5C.PI and cone cells are wild-type, the cone cells cluster in a 'cruciform' shape indistinguishable from that due to CadN^M19/CadN^M19. clones. However, when only one of the two primary pigment cells in an otherwise wild-type ommatidium is CadN^Scer\UAS.cIa; Scer\GAL4^Act5C.PI, there are major changes in cone cell shape and arrangement: the cone cells selectively adhere to the CadN^Scer\UAS.cIa; Scer\GAL4^Act5C.PI primary pigment cell which can be altered into a variety of shapes, often engulfing one or more of the cone cells.

(Hayashi and Carthew, 2004)

Expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^GH146 in either neuroblast or single-cell DL1 projection neuron clones (induced using the MARCM technique) do not result in detectable phenotypes in the dendrites of the mutant neurons. There is no change in the axonal branches of the mutant neuron in the mushroom body, but in the lateral horn in 47% of mutant neurons, instead of one dorsal axon branch, there are two dorsal branches.

(Zhu and Luo, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

CadN^UAS.cIa, Scer\GAL4^hs.2sev has abnormal cell polarity phenotype, enhanceable by arm^{S2.UAS.Tag:MYC}, Scer\GAL4^hs.2sev

(Mirkovic and Mlodzik, 2006)

Suppressed by

Statement

Reference

CadN^UAS.cIa, Scer\GAL4^hs.2sev has abnormal cell polarity phenotype, suppressible | partially by arm⁴/arm[+]

(Mirkovic and Mlodzik, 2006)

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/CadN^UAS.cIa is an enhancer of abnormal neuroanatomy | pupal stage | cell autonomous | somatic clone phenotype of Lar²¹²⁷

(Prakash et al., 2009)

Scer\GAL4^elav-C155/CadN^UAS.cIa is an enhancer of abnormal neuroanatomy | pupal stage | somatic clone phenotype of Liprin-α¹

(Prakash et al., 2009)

CadN^UAS.cIa, Scer\GAL4^hs.2sev is an enhancer of abnormal cell polarity phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

NOT Enhancer of

Statement

Reference

CadN^UAS.cIa, Scer\GAL4^hs.2sev is a non-enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^tj.PU/CadN^UAS.cIa is a suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa is a non-suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^GH146/CadN^UAS.cIa is a non-suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of meigo¹

(Sekine et al., 2013)

CadN^UAS.cIa, Scer\GAL4^hs.2sev is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

CadN^UAS.cIa has ommatidium phenotype, enhanceable by Scer\GAL4^hs.2sev/arm^{S2.UAS.Tag:MYC}

(Mirkovic and Mlodzik, 2006)

Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/CadN^UAS.cIa is an enhancer of photoreceptor neuron | pupal stage | cell autonomous | somatic clone phenotype of Lar²¹²⁷

(Prakash et al., 2009)

Scer\GAL4^elav-C155/CadN^UAS.cIa is an enhancer of photoreceptor neuron | pupal stage | somatic clone phenotype of Liprin-α¹

(Prakash et al., 2009)

CadN^UAS.cIa, Scer\GAL4^hs.2sev is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, shg^{dCR3h.UAS.sgGFP}

(Mirkovic and Mlodzik, 2006)

NOT Enhancer of

Statement

Reference

CadN^UAS.cIa, Scer\GAL4^hs.2sev is a non-enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Suppressor of

Statement

Reference

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor | partially of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

CadN^UAS.cIa/Scer\GAL4^upd1.PU is a suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^tj.PU/CadN^UAS.cIa is a suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^tj.PU/CadN^UAS.cIa is a suppressor | partially of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^tj.PU/CadN^UAS.cIa is a suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa is a non-suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa is a non-suppressor of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}/CadN^UAS.cIa is a non-suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

Scer\GAL4^GH146/CadN^UAS.cIa is a non-suppressor of adult antennal lobe projection neuron | somatic clone phenotype of meigo¹

(Sekine et al., 2013)

CadN^UAS.cIa, Scer\GAL4^hs.2sev is a non-suppressor of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Other

Statement

Reference

CadN^UAS.cIa, Scer\GAL4^hs.2sev, arm⁴/arm[+] has ommatidium phenotype

(Mirkovic and Mlodzik, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of CadN^Scer\UAS.cIa under the control of either Scer\GAL4^upd1.PU or Scer\GAL4^tj.PU fully rescues the decreased cell numbers of hub cells and cyst stem cells, and partially rescues the decreased number of germline stem cells, in the adult testicular niche of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of sda^iso7.8/Df(3R)ED6235 mutants.

(Lim et al., 2015)

Expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^elav-C155 in Lar²¹²⁷ somatic mutant clones enhances the photoreceptor targeting defects observed in single mutants.

Expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^elav-C155 in Liprin-α¹ somatic mutant clones enhances the photoreceptor targeting defects.

(Prakash et al., 2009)

The under-rotation of ommatidia seen in Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} eyes is enhanced by coexpression of CadN^Scer\UAS.cIa.

The ommatidial rotation phenotype of Scer\GAL4^hs.2sev>CadN^Scer\UAS.cIa mutants is partially suppressed in an arm⁴/+ background but gaps appear between ommatidia due to decreased adhesion. Coexpression of arm^{S2.Scer\UAS.T:Hsap\MYC} enhances the ommatidial rotational defects of Scer\GAL4^hs.2sev>CadN^Scer\UAS.cIa mutants and causes the loss of some photoreceptors.

(Mirkovic and Mlodzik, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155/CadN^UAS.cIa rescues CadN^unspecified

(Hummel and Zipursky, 2004)

Scer\GAL4^GH146/CadN^UAS.cIa rescues CadN^M19

(Zhu and Luo, 2004)

Scer\GAL4^sca-537.4/CadN^UAS.cIa rescues CadN^M19

(Iwai et al., 1997)

Partially rescues

Scer\GAL4^Tub.PU/CadN^UAS.cIa partially rescues CadN⁴⁰⁵

(Nern et al., 2005)

Scer\GAL4^elav-C155/CadN^UAS.cIa partially rescues CadN⁴⁰⁵

(Ting et al., 2005)

Comments

The mistargeting and growth cone morphology defects seen in CadN⁴⁰⁵ mutant R7 cells at 17% and 35% APF are substantially rescued by expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^elav-C155.

(Ting et al., 2005)

The abnormal phenotypes seen in the antennal lobe, mushroom body calyx and lateral horn in homozygous CadN^M19 projection neurons are rescued by expression of CadN^Scer\UAS.cIa under the control of Scer\GAL4^GH146.

(Zhu and Luo, 2004)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CadN^Scer\UAS.cIa

CadN^UAS.cIa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Iwai

(Iwai et al., 1997)

Secondary FlyBase IDs

References (19)

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