FB2024_03 , released June 25, 2024
Allele: Scer\GAL4bsh.M.PT
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General Information
Symbol
Scer\GAL4bsh.M.PT
Species
S. cerevisiae
Name
brain-specific homeobox promoter construct of Trush
FlyBase ID
FBal0358478
Feature type
allele
Associated gene
Scer\GAL4
Associated Insertion(s)
Carried in Construct
P{bsh-GAL4.M.T}
Also Known As
bshM-Gal4
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Trush et al., 2019)
Progenitor genotype
Carried in construct
P{bsh-GAL4.M.T}
Cytology
Description

The 1.4kb bsh short promoter (combined with 2kb bshD and 378bp bshPB fragments) drives expression of GAL4. IVS-Syn21-p10 is also present (a small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene, along with the 3' UTR from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene).

(Trush et al., 2019)
Allele components
Component
Use(s)
Regulatory region(s)
bsh
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter (Gal4 UAS)
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      third instar larval stage -- pharate adult stage P8
      medullary intrinsic neuron Mi1
      (Trush et al., 2019)
      Additional Information
      Statement
      Reference

      ScerGAL4bsh.M.PT drives expression in medullary intrinsic neuron Mi1 at from third instar larval stage through 48 hours APF. At pupal stages, Mi1 neurons project to medullary layers M1-M2.

      (Trush et al., 2019)
       
      Marker for
      Reflects expression of
      bsh
      (Trush et al., 2019)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      axon terminus | larval stage, with CadNUAS.cIa

      (Trush et al., 2019)

      axon terminus | larval stage | somatic clone, with CadNUAS.cIa

      (Trush et al., 2019)

      medulla | larval stage, with CadNUAS.cIa

      (Trush et al., 2019)

      medulla | larval stage | somatic clone, with CadNUAS.cIa

      (Trush et al., 2019)

      medulla | P-stage, with dshΔDEP+.UAS

      (Han et al., 2020)

      medulla | P-stage, with fz2ECD.GPI.UAS.Tag:MYC

      (Han et al., 2020)

      medulla | P-stage | somatic clone, with fz2JF01259

      (Han et al., 2020)

      medulla | P-stage | somatic clone, with fzHMS01308

      (Han et al., 2020)

      medulla | P-stage | somatic clone, with Wnt4UAS.cGa

      (Han et al., 2020)

      medulla | P-stage | somatic clone, with Wnt10UAS.3x.Tag:HA

      (Han et al., 2020)

      medullary intrinsic neuron Mi1 | larval stage, with CadNUAS.cIa

      (Trush et al., 2019)

      medullary intrinsic neuron Mi1 | larval stage | somatic clone, with CadNUAS.cIa

      (Trush et al., 2019)

      medullary intrinsic neuron Mi1 | P-stage | somatic clone, with dshΔDEP+.UAS

      (Han et al., 2020)

      medullary intrinsic neuron Mi1 | P-stage | somatic clone, with fz2JF01259

      (Han et al., 2020)

      medullary intrinsic neuron Mi1 | P-stage | somatic clone, with fzHMS01308

      (Han et al., 2020)

      medullary intrinsic neuron Mi1 | P-stage | somatic clone, with Wnt4UAS.cGa

      (Han et al., 2020)

      medullary intrinsic neuron Mi1 | P-stage | somatic clone, with Wnt10UAS.3x.Tag:HA

      (Han et al., 2020)

      photoreceptor cell R8 | P-stage | somatic clone, with dshΔDEP+.UAS

      (Han et al., 2020)

      photoreceptor cell R8 | P-stage | somatic clone, with fz2JF01259

      (Han et al., 2020)

      photoreceptor cell R8 | P-stage | somatic clone, with fzHMS01308

      (Han et al., 2020)
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Scer\GAL4bsh.M.PT
      Name Synonyms
      brain-specific homeobox promoter construct of Trush
      Secondary FlyBase IDs
        References (3)