FB2024_03 , released June 25, 2024
Recombinant Construct: P{bsh-GAL4.M.T}
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General Information
Symbol
P{bsh-GAL4.M.T}
FlyBase ID
FBtp0141249
Feature type
transgenic_transposable_element
Component Allele(s)
Scer\GAL4bsh.M.PT
Also Known As
bshM-Gal4
Size
Expression Data
GAL4 reporter/driver
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component allele Scer\GAL4bsh.M.PT
Transgenic product class
Component
Use(s)
Regulatory region(s)
bsh
Encoded product / tool
GAL4
Description

The 1.4kb bsh short promoter (combined with 2kb bshD and 378bp bshPB fragments) drives expression of GAL4. IVS-Syn21-p10 is also present (a small intron derived from the Mhc gene followed by a synthetic AT-rich 21bp sequence made by combining the Cavener consensus sequence with elements from the Malacosoma neustria nucleopolyhedrovirus (MnNPV) polyhedrin gene, along with the 3' UTR from the Autographa californica nucleopolyhedrovirus (AcNPV) p10 gene).

(Trush et al., 2019)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage -- pharate adult stage P8
medullary intrinsic neuron Mi1
(Trush et al., 2019)
Additional Information
Statement
Reference

ScerGAL4bsh.M.PT drives expression in medullary intrinsic neuron Mi1 at from third instar larval stage through 48 hours APF. At pupal stages, Mi1 neurons project to medullary layers M1-M2.

(Trush et al., 2019)
 
Marker for
Reflects expression of
bsh
(Trush et al., 2019)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Mi1-Gal4
(Lee et al., 2023)
P{bsh-GAL4.M.T}
(FlyBase, 1992-)
bshM-Gal4
(Lee et al., 2023, Han et al., 2020, Trush et al., 2019)
Secondary FlyBase IDs
    References (4)