FlyBase Allele Report: Dmel\shot[k03405]

General Information

Symbol

Dmel\shot^k03405

Species

D. melanogaster

Name

FlyBase ID

FBal0064571

Feature type

allele

Associated gene

Dmel\shot

Associated Insertion(s)

P{lacW}shot^k03405

Carried in Construct

Also Known As

shot^kakP2, l(2)k03405, kak^P2

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Bottenberg et al., 2009)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}shot^k03405

Cytology

Description

An insertion of a transposable element into the intron between the second and third transcriptional start sites of shot.

(Bottenberg et al., 2009)

An insertion of a transposable element into the intron between the second and third transcriptional start sites of shot. The P-element is inserted at genomic position 9,807,300 (accession number FJ869115).

(Bottenberg et al., 2009)

P{lacW} insertion in an intron 1917bp before the first exon common to mRNAs encoding shot isoforms A and B.

(Lee et al., 2000)

Insertion of a P{lacW} element into the intron at the 5' end of the gene that separates the alternate starts from the shared exons.

(Gregory and Brown, 1998)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,919,795..13,919,795

(Qu et al., 2022, Ricolo and Araujo, 2020, Bottenberg et al., 2009, Lee et al., 2000, Spradling et al., 1999, Gregory and Brown, 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Df(2R)MK1)

(Sanchez-Soriano et al., 2009)

lethal | recessive

(Spradling et al., 1999, Gregory and Brown, 1998, Prokop et al., 1998)

Phenotype Manifest In

actin cytoskeleton | embryonic stage

(Ricolo and Araujo, 2020)

axon & motor neuron | embryonic stage

(Bottenberg et al., 2009)

dendrite & motor neuron | embryonic stage

(Bottenberg et al., 2009)

embryo | dorsal closure stage

(Gregory and Brown, 1998)

embryonic epidermis

(Gregory and Brown, 1998)

microtubule (with Df(2R)MK1)

(Sanchez-Soriano et al., 2009)

microtubule | embryonic stage

(Ricolo and Araujo, 2020)

mitotic domain 1 | extended germ band stage

(Gregory and Brown, 1998)

neuron (with Df(2R)MK1)

(Sanchez-Soriano et al., 2009)

terminal tracheal cell (with shot^ΔEGC)

(Ricolo and Araujo, 2020)

terminal tracheal cell | embryonic stage

(Ricolo and Araujo, 2020)

tracheal lumen (with shot^ΔEGC)

(Ricolo and Araujo, 2020)

tracheal lumen | embryonic stage

(Ricolo and Araujo, 2020)

Detailed Description

Statement

Reference

shot^k03405 mutant embryos display no muscle detachment phenotypes.

The dendrites of motorneurons are strongly reduced and motorneuron axons stall in shot^k03405 mutant embryos.

(Bottenberg et al., 2009)

shot^k03405/Df(2R)MK1 primary neuronal cultures show a significantly reduced axonal length compared to controls. They also show an increase of neurons with unbundled microtubules - these microtubules display irregular trajectories and frequent loops in axons and growth cones.

shot³/Df(2R)MK1 primary neuronal cultures have normal numbers of filopodia.

(Sanchez-Soriano et al., 2009)

Germ band retraction and head involution fail in approximately 15% of homozygous embryos. Breaks in the ventral epidermis of the middle abdominal segments are seen in fully germ band extended embryos.

(Gregory and Brown, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions