shot^HG25 mutants show strong reduction of output synapses at the NMJ, while this phenotype is not seen in the CNS.

Homozygous and hemizygous embryos show a paralytic phenotype. Neuromuscular junctions occupy far less surface of their respective muscles, their branches are reduced in length and boutons appear reduced in number and size in hemizygous and shot^HG25/shot^91k embryos compared to wild-type. Peripheral nerves can form correctly in shot^91k/shot^HG25 embryos. The short SNb-branch has a tendency to stall in these embryos. shot^91k/shot^HG25 embryos do not show any obvious defects in muscle patterning at stage 16. However, at stage 17, severe detachment of the muscles from the cuticle is seen, although the muscles remain attached to each other.