FlyBase Allele Report: Dmel\shot[k03010]

General Information

Symbol

Dmel\shot^k03010

Species

D. melanogaster

Name

FlyBase ID

FBal0064572

Feature type

allele

Associated gene

Dmel\shot

Associated Insertion(s)

P{lacW}shot^k03010

Carried in Construct

Also Known As

l(2)k03010, shot^kakP1, kak^P1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}shot^k03010

Cytology

Description

P{lacW} insertion in an intron 1917bp before the first exon common to mRNAs encoding shot isoforms A and B.

(Lee et al., 2000)

Insertion of a P{lacW} element into the intron at the 5' end of the gene that separates the alternate starts from the shared exons.

(Gregory and Brown, 1998)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,919,803..13,919,803 [+]

(Lee et al., 2000, Spradling et al., 1999, Gregory and Brown, 1998, Strumpf and Volk, 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Lee et al., 2000)

decreased size | oogenesis, with Scer\GAL4^{VP16.mat.αTub67C}, shot^RNAi.UAS.ABD

(Lu et al., 2021)

lethal | recessive

(Spradling et al., 1999, Gregory and Brown, 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

shot^k03010/Df(2R)BSC361 transheterozygotes do not display visible microtubule distribution defects in third instar larval adipocytes.

(Sun et al., 2019)

shot^k03010 homozygous and shot^k03010/shot^ΔEGC transheterozygous embryos show a smaller dorsal hole width during dorsal closure, as shown by a significant increase in the length:width ratio, and the leading edge of their dorsal-most epithelial cells do not display significant changes in cell size, but present a significant decrease in the number of filopodia, present frequent long apical protrusions and present frequent abnormally long, bent and protruding microtubules, as compared to controls.

(Takács et al., 2017)

When homozygous germ-line clones are made, no defect is seen in oocyte determination.

(Roper and Brown, 2004)

The lateral chordotonal sensory axons arrest at variable stages before reaching the central nervous system (CNS); 27% extend towards the intersegmental nerve (ISN) and stall or fail to reach the ISN, 55% travel a significant distance within the ISN, 18% reach the CNS but do not penetrate it, and none of the axons enter the CNS and form arborisations.

(Lee et al., 2000)

In the dorsal cluster of embryonic dendrites of shot³⁰/shot^k03010 embryos, the number of anterior lateral branches is reduced from about 10 to 4 per hemisegment, whereas more branches extend dorsally and appear to contact each other more extensively.

(Gao et al., 1999)

Germ band retraction and head involution fail in approximately 15% of homozygous embryos.

(Gregory and Brown, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

shot^k04204

(BDGP Project Members, 1994-1999)

Fails to complement

shot³⁰

(Gao et al., 1999)

shot^k03405

(BDGP Project Members, 1994-1999)

shot^k06601

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10522

y¹ w^67c23; P{lacW}shot^k03010/CyO

Kyoto

102250

y¹ w^67c23 ; P{lacW}shot^k03010 / CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Lethality is reverted by excision of the P{lacW} element.

(Gregory and Brown, 1998)

The site of the P{lacW} insertion in shot^k03010 is identical to the site of the P{lacW} insertion in shot^k03405.

(Lee et al., 2000)

Complements: Lac^k11012b.

(Beitel and Krasnow, 2000)

Complements: mam⁰⁴⁶¹⁵. Complements: l(2)k08708^k08708.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

kak^P1

(Lee et al., 2000, Gregory and Brown, 1998)

kak^k03010

(Gao et al., 1999)

l(2)k03010

(Lee et al., 2000, Spradling et al., 1999, Gregory and Brown, 1998, Strumpf and Volk, 1998)

l(2)k03010^k03010

short stop^k3010

(Beitel and Krasnow, 2000)

shot^k03010

shot^kakP1

(Takács et al., 2017, Roper and Brown, 2004, Roper and Brown, 2003)

shot^ΔABD

(Lu et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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