FlyBase Allele Report: Dmel\stck[3R-17]

General Information

Symbol

Dmel\stck^3R-17

Species

D. melanogaster

Name

FlyBase ID

FBal0063753

Feature type

allele

Associated gene

Dmel\stck

Associated Insertion(s)

Carried in Construct

Also Known As

stck¹⁷

Key Links

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Kadrmas et al., 2004, Kadrmas et al., 2004, Clark et al., 2003)

Mutagen

X ray

(Prout et al., 1997)

Progenitor genotype

Cytology

Description

571bp deletion in stck encompassing nucleotides 2095-2664.

(Clark et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | embryonic stage (with stck¹⁸)

(Elias et al., 2012)

lethal | larval stage (with stck¹⁸)

(Elias et al., 2012)

lethal - all die before end of larval stage | recessive

(Prout et al., 1997)

some die during larval stage | recessive

(Prout et al., 1997)

viable (with stck¹⁸), with stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

visible (with stck¹⁸), with stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

visible | recessive | somatic clone

(Clark et al., 2003)

visible | somatic clone

(Prout, 1997.3.19, Prout et al., 1997)

Phenotype Manifest In

embryonic/first instar larval cuticle | dorsal | germline clone

(Kadrmas et al., 2004, Kadrmas et al., 2004)

embryonic/larval somatic muscle cell

(Prout et al., 1997)

embryonic head | germline clone

(Kadrmas et al., 2004)

embryonic somatic muscle cell (with Df(3R)097)

(Clark et al., 2003)

larva

(Prout et al., 1997)

myofibril & actin filament (with Df(3R)097)

(Clark et al., 2003)

wing (with stck¹⁸), with stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

wing | somatic clone

(Clark et al., 2003, Prout, 1997.3.19, Prout et al., 1997)

Detailed Description

Statement

Reference

stck^3R-17/+ does not significantly affect resistance to ethanol-induced sedation.

(Ojelade et al., 2015)

stck^3R-17/stck¹⁸ adults rescued by stck^{D303V.T:Zzzz\FLAG} are recovered at 90% of the expected frequency. The rescued adults frequently have wing blisters and wings are often seen in a "held up" position.

(Pronovost et al., 2013)

Embryos expressing stck^{wt.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background do not display any muscle structure or actin cytoskeleton defects.

Embryos expressing stck^{Q38A.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background do not display any muscle structure or actin cytoskeleton defects.

(Elias et al., 2012)

stck^3R-17 dorsal branch terminal cell clones do not cause any detectable defects in terminal cell morphology.

(Levi et al., 2006)

Embryos derived from stck^3R-17 germ-line clones and zygotically mutant for stck^3R-17 display dorsal closure defects, ventral patterning defects and/or head involution defects.

(Kadrmas et al., 2004)

Embryos derived from females carrying stck^3R-17 germ-line clones have cuticles with dorsal puckers (36%), dorsal holes (23%), or wild type morphology (12%). Cuticles from embryos both maternally and zygotically stck^3R-17 mutant show the same array of phenotypes.

(Kadrmas et al., 2004)

Actin filament arrays are largely retracted to one end of the muscle in stck^3R-17/Df(3R)097 late stage 17 embryos. Homozygous clones result in blisters in the wing.

(Clark et al., 2003)

Homozygotes die as early larvae. Homozygous clones in the wing produce discrete, round blisters of variable size. These blisters can be located anywhere on the wing. Wing venation is normal. Homozygous embryos show excessive contractions of the segmental muscles just before hatching. Hatched larvae fail to grow.

(Prout et al., 1997)

Homozygous clones in the wing produce a blistered phenotype.

(Prout, 1997.3.19)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

stck[+]/stck^3R-17 is an enhancer of lethal phenotype of flw^G0172

(Pronovost et al., 2013)

stck[+]/stck^3R-17 is an enhancer of visible | recessive phenotype of mys⁸

(Prout et al., 1997)

stck[+]/stck^3R-17 is an enhancer of visible | recessive phenotype of mys^b9

(Prout et al., 1997)

Suppressor of

Statement

Reference

stck[+]/stck^3R-17 is a suppressor of chemical resistant | adult stage phenotype of Scer\GAL4^elav.PU, ics^RNAi.UAS.WIZ

(Ojelade et al., 2015)

Other

Statement

Reference

ics^BG02577, stck¹⁸/stck^3R-17, stck^{Q38A.Tag:FLAG} has partially lethal - majority die phenotype

(Elias et al., 2012)

ics^BG02577, stck¹⁸/stck^3R-17, stck^{Q38A.Tag:FLAG} has abnormal developmental rate | first instar larval stage phenotype

(Elias et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

stck[+]/stck^3R-17 is an enhancer of wing phenotype of mys⁸

(Prout et al., 1997)

stck[+]/stck^3R-17 is an enhancer of wing phenotype of mys^b9

(Prout et al., 1997)

NOT Suppressor of

Statement

Reference

stck^3R-17 is a non-suppressor of phenotype of arm³

(Cox et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

stck^3R-17/+ significantly suppresses (to wild type levels) the increased resistance to ethanol-induced sedation seen in flies with expression of ics^{dsRNA.Scer\UAS.WIZ} driven by Scer\GAL4^elav.PU.

(Ojelade et al., 2015)

flw^G0172 ; stck^3R-17 /+ animals show complete larval lethality (only a limited number of animals were of this genotype could be isolated due to a high rate of balancer breakdown in the required crosses).

(Pronovost et al., 2013)

ics^BG02577 has no effect on the viability of flies expressing stck^{wt.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background.

The addition of ics^BG02577 reduces the viability of flies expressing stck^{Q38A.T:Zzzz\FLAG} in a stck^3R-17/stck¹⁸ mutant background. Between 6 and 34% of the expected number of progeny are seen, with the majority of the lethality taking place during the larval and pharate stages. The rescued larvae display a range of sizes, including presumptive first instar larvae that appear stumpy, exhibit sluggish movement and do not progress in development.

(Elias et al., 2012)

Shows a weak genetic interaction with mys⁸ and mys^b9; the frequency of held-out wings but not the frequency of blisters in mys⁸ or mys^b9 single hemizygotes is increased if they are also heterozygous for stck^3R-17.

(Prout et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

stck¹⁸/stck^3R-17 is rescued by stck^Tag:FLAG

(Yoshigi et al., 2013)

stck¹⁸/stck^3R-17 is rescued by stck^wt.Tag:FLAG

(Elias et al., 2012)

stck¹⁸/stck^3R-17 is rescued by stck^{Q38A.Tag:FLAG}

(Elias et al., 2012)

Partially rescued by

stck¹⁸/stck^3R-17 is partially rescued by stck^{D303V.Tag:FLAG}

(Pronovost et al., 2013)

Comments

Expression of stck^{wt.T:Zzzz\FLAG} rescues the embryonic and larval lethality seen in stck^3R-17/stck¹⁸ mutants. The rescued flies do not display any overt phenotypes.

Expression of stck^{Q38A.T:Zzzz\FLAG} rescues the embryonic and larval lethality seen in stck^3R-17/stck¹⁸ mutants. The rescued flies are fertile and do not display either wing blisters or any other overt phenotypes.

(Elias et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2302

y¹ w¹; P{neoFRT}82B stck^3R-17 P{white-un1}90E/TM6B, P{iab-2(1.7)lacZ}6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

stck¹⁷

(Pronovost et al., 2013, Yoshigi et al., 2013, Elias et al., 2012, Kadrmas et al., 2004, Kadrmas et al., 2004, Clark et al., 2003)

stck^3R-17

(Thuveson et al., 2019, Ojelade et al., 2015, Knox et al., 2011, Levi et al., 2006)

stk^3R-17

(Prout, 1997.3.19)

Name Synonyms

Secondary FlyBase IDs

References (14)

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