FlyBase Allele Report: Dmel\hh[UAS.cIa]

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^vg.int2.1 results in extensive overproliferation of anterior compartment cells in the wing disc.

(Ibrahim et al., 2013)

Flies overexpressing hh^Scer\UAS.cIa under the control of Scer\GAL4^Hml.Δ in the hemocytes develop normally, with no obvious defects after they hatch. Expression of hh^Scer\UAS.cIa does not protect against infection with group B streptococcus.

(Wang et al., 2011)

Expression of hh^Scer\UAS.cIa, under the control of two copies of Scer\GAL4^sim.PS, causes cell death in embryos.

Expression of hh^Scer\UAS.cIa, under the control of Scer\GAL4^sca-537.4, abolishes the formation of the anterior commissure, but not of the posterior commissure. The differentiation of midline glia and MP1 neurons is also affected.

hh^Scer\UAS.cIa expression in all midline cells from late stage 9, driven by Scer\GAL4^sim.PS, results in a severe reduction in cell number. The few remaining axons of the surviving midline cells suggest that the UMI and VUM interneurons are still able to differentiate.

When cells expressing hh^Scer\UAS.cIa, under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16}, are transplanted close to the midline, the differentiation of MP1 neurons is disrupted. The resulting lack of MP1 axons causes a gap in the MP1 axon fascicle. In some embryos, axons project randomly across the longitudinal tracts. The number of midline cells in the affected neuromeres is not reduced, although the midline glia are occasionally absent or pushed out of the CNS. The axons of the VUM interneurons defasciculate slightly. All other midline cell lineages develop normally.

(Bossing and Brand, 2006)

Overexpression of hh^Scer\UAS.cIa under the control of Scer\GAL4^lz-gal4 behind the morphogenetic furrow results in mildly disordered bristles and ommatidial packing. The pupal retinas show mild defects near one edge, with occasional loss or excess cone cells (4%), mis-oriented of incompletely wrapped primary cells (44%) and excess lattice cells (55%). Photoreceptor differentiation, assessed by counting rhabdomeres in pupal retinas, is unaffected in these mutants.

(Kent et al., 2006)

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^dpp.3KK results in partial loss of wing vein L3.

(Molnar et al., 2006)

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^pb.PJ does not affect labial palp development.

(Joulia et al., 2005)

The cuticles formed by hh^Scer\UAS.cIa; Scer\GAL4^69B embryos have reduced denticle diversity: rows 3-5 adopt row 2 identity (there is often an extra row 2 in addition to these), while row 6 adopts row 1 identity.

(Gallet et al., 2003)

When hh^Scer\UAS.cIa is driven by Scer\GAL4^69B in the embryo a mirror image duplication of anterior denticles is seen in the abdominal area. The amount of naked cuticle seen is reduced compared to wild-type.

(Angelats et al., 2002)

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^h-1J3 results in germ cell migration defects in the embryo. These defects are evident just after the germ cells leave the posterior midgut; instead of migrating dorsally towards the overlying mesoderm, some germ cells in stage 10-11 embryos remain associated with the posterior midgut or migrate in the opposite direction towards the ventral side of the embryo. In stage 13 embryos, germ cells are seen at a variety of abnormal positions in the posterior ectoderm in addition to the normal cluster of germ cells in the mesoderm near the posterior of the embryo. Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^elav.PLu results in abnormal germ cell migration patterns in the embryo. Two clusters of germ cells on either side of the ventral midline can be seen in these embryos at stage 13, but in contrast to wild-type embryos the cells within the clusters are not tightly associated with each other and some are dispersed at distant sites in the posterior mesoderm. In addition, some germ cells are typically found near the ventral midline in the region just underlying the central nervous system. Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^twi.PG results in germ cell migration defects in the embryo. In these embryos at stage 12-13, germ cells are found in several small clusters of 3-6 cells scattered in different segments along the anterior-posterior axis. In stage 14-15 embryos, many of the germ cells are clustered near the very posterior of the embryo. Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^how-24B results in germ cells congregating in the region of the mesoderm just posterior to the midgut invagination (the site of ectopic hh expression) in stage 11 embryos.

(Deshpande et al., 2001)

Embryos in which hh^Scer\UAS.cIa is expressed under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} have a deranged overall morphology, but the tracheal cells are capable of forming all the branches except the dorsal branch. The morphology of the branches is slightly affected but primary patterning proceeds normally. Embryos in which hh^Scer\UAS.cIa is expressed under the control of Scer\GAL4^btl.PS have almost normal branching of the primary and secondary tracheal branches, with occasional defects in the dorsal branch and lateral trunk posterior. Excess terminal cells and excess terminal branching are seen in the dorsal branch.

(Glazer and Shilo, 2001)

Embryos expressing hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1 show mutant phenotypes in every other segment. Thoracic and the first abdominal segment are principally covered by naked cuticle, while abdominal segments 3, 5 and 7 show mirror image duplication of their denticle belts, showing duplication of denticle rows 1 and 2.

(Gallet et al., 2000)

Ectopic expression of hh^Scer\UAS.cIa by Scer\GAL4^dpp.blk1 causes severely disrupted eye-antennal disc morphology.

(Amin et al., 1999)

When expression is driven by Scer\GAL4^en-e16E the denticle belts are affected such that there is an excess of small denticles, of type row 2-4, at the expense of rows 5 and 6.

(Gritzan et al., 1999)

Flies expressing hh^Scer\UAS.cIa under the control of Scer\GAL4^en-e16E show anterior displacement of wing vein 3 and a more anterior restriction of the double row bristles, leading to an expansion of the intervein 3-4 region.

(Alves et al., 1998)

Scer\GAL4^30A driven expression causes dramatic respecification of the pupal wing anterior compartment, costa is eliminated and triple row bristles are present on most of the anterior wing margin are replaced by double row bristles. Pupae also exhibit duplication of notal structures. Scer\GAL4^34B driven expression causes suppression of the costa and overgrowth in the anterior wing compartment resulting in a bulge in the anterior margin and an increase in the distance between veins II and III.

(Ingham and Fietz, 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/ci^KK100760

(Gore et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^NP2222, hh^UAS.cIa has decreased occurrence of cell division | larval stage phenotype, suppressible by Lsd-2^HMS01292, Scer\GAL4^NP2222

(Dong et al., 2021)

Scer\GAL4^NP2222, hh^UAS.cIa has decreased occurrence of cell division | larval stage phenotype, suppressible by Lsd-2^HMS00629, Scer\GAL4^NP2222

(Dong et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/disp^GD2813

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, suppressible | partially by dpp^d12

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, suppressible | partially by dpp^d14

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, suppressible | partially by tkv⁷

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, suppressible | partially by tkv⁸

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, suppressible | partially by Rab8^GD12520, Scer\GAL4^hh-Gal4

(Gore et al., 2021)

Scer\GAL4^en-e16E, hh^UAS.cIa has visible phenotype, suppressible by fu¹

(Alves et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^hh-Gal4, hh^UAS.cIa has increased size | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/ci^KK100760

(Gore et al., 2021)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, non-enhanceable by Scer\GAL4^hh-Gal4/ci^KK100760

(Gore et al., 2021)

Suppressed by

Statement

Reference

Scer\GAL4^NP2222, hh^UAS.cIa has neuroblast | larval stage phenotype, suppressible by Lsd-2^HMS01292, Scer\GAL4^NP2222

(Dong et al., 2021)

Scer\GAL4^NP2222, hh^UAS.cIa has neuroblast | larval stage phenotype, suppressible by Lsd-2^HMS00629, Scer\GAL4^NP2222

(Dong et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, suppressible | partially by Scer\GAL4^hh-Gal4/disp^GD2813

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, suppressible | partially by dpp^d14

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, suppressible | partially by dpp^d12

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, suppressible | partially by tkv⁸

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, suppressible | partially by tkv⁷

(Gore et al., 2021)

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, suppressible | partially by Rab8^GD12520, Scer\GAL4^hh-Gal4

(Gore et al., 2021)

Scer\GAL4^en-e16E, hh^UAS.cIa has denticle | germline clone | maternal effect phenotype, suppressible | partially by disp^S037707

(Gallet et al., 2003)

Scer\GAL4^en-e16E, hh^UAS.cIa has denticle | germline clone | maternal effect phenotype, suppressible | partially by ttv^00681b

(Gallet et al., 2003)

Scer\GAL4^prd.RG1, hh^UAS.cIa has embryonic abdominal segment 5 & denticle belt | supernumerary phenotype, suppressible by ci⁹⁴

(Gallet et al., 2000)

Scer\GAL4^prd.RG1, hh^UAS.cIa has embryonic abdominal segment 7 & denticle belt | supernumerary phenotype, suppressible by ci⁹⁴

(Gallet et al., 2000)

Scer\GAL4^prd.RG1, hh^UAS.cIa has embryonic abdominal segment 3 & denticle belt | supernumerary phenotype, suppressible by ci⁹⁴

(Gallet et al., 2000)

Scer\GAL4^en-e16E, hh^UAS.cIa has 1st posterior cell phenotype, suppressible by fu¹

(Alves et al., 1998)

Scer\GAL4^en-e16E, hh^UAS.cIa has wing vein L3 phenotype, suppressible by fu¹

(Alves et al., 1998)

Scer\GAL4^en-e16E, hh^UAS.cIa has dorsal row of double row of wing sensilla phenotype, suppressible by fu¹

(Alves et al., 1998)

Scer\GAL4^en-e16E, hh^UAS.cIa has ventral row of double row of wing sensilla phenotype, suppressible by fu¹

(Alves et al., 1998)

NOT suppressed by

Statement

Reference

Scer\GAL4^hh-Gal4, hh^UAS.cIa has wing disc anterior compartment | larval stage phenotype, non-suppressible by Scer\GAL4^hh-Gal4/ci^KK100760

(Gore et al., 2021)

Suppressor of

Statement

Reference

Scer\GAL4^unspecified/hh^UAS.cIa is a suppressor of embryonic/first instar larval cuticle | germline clone phenotype of disp^S037707

(Gallet et al., 2006)

Scer\GAL4^prd.RG1/hh^UAS.cIa is a suppressor of embryonic/first instar larval cuticle phenotype of sll^7E18

(Luders et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^prd.RG1/hh^UAS.cIa is a non-suppressor of embryonic/first instar larval cuticle phenotype of dlp^A187

(Yan et al., 2010)

Other

Statement

Reference

Scer\GAL4^en-e16E, disp^S037707, hh^UAS.cIa has denticle | germline clone | maternal effect phenotype

(Gallet et al., 2003)

Scer\GAL4^en-e16E, hh^UAS.cIa, ttv^00681b has denticle | germline clone | maternal effect phenotype

(Gallet et al., 2003)

Scer\GAL4^prd.RG1, hh^UAS.cIa, tsh⁸ has ventral denticle belt phenotype

(Gallet et al., 2000)

Scer\GAL4^e22c, hh^UAS.cIa, ptc^G12 has ventral denticle belt phenotype

(Quirk et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1 does not restore naked cuticle in dlp^A187 embryos derived from dlp^A187 female germline clones.

(Yan et al., 2010)

Expression of hh^Scer\UAS.cIa, under the control of Scer\GAL4^unspecified in disp^S037707 mutant embryos rescues some type 1 cuticle, but no type 2 or type 1 cuticle is formed.

(Gallet et al., 2006)

Expression of hh^Scer\UAS.cIa with Scer\GAL4^en-e16E in disp^S037707 germ-line clone embryos doesn't rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: row 3,4 and 5 type denticles are all present. Expression of hh^Scer\UAS.cIa with Scer\GAL4^en-e16E in ttv^00681b germ-line clone embryos doesn't rescue naked cuticle formation, but does increase denticle diversity in the ventral cuticle: denticles corresponding to row 2,3,4, and 5 types are all present.

(Gallet et al., 2003)

hh^Scer\UAS.cIa; Scer\GAL4^prd.RG1 fails to rescue metameric furrow formation in Df(2R)en^E; wg^l-17 double homozygous embryos.

(Larsen et al., 2003)

When wg^Scer\UAS.cKa or hh^Scer\UAS.cIa is driven by Scer\GAL4^prd.RG1 in sll^7E18 animals suppression of the segment polarity phenotype is seen in those domains that express Scer\GAL4^prd.RG1.

(Luders et al., 2003)

When hh^Scer\UAS.cIa is expressed under the control of Scer\GAL4^prd.RG1 in a an embryo that is zygotically and maternally mutant for frc⁰⁰⁰⁷³, naked cuticle is restored in a typical pair-rule pattern.

(Selva et al., 2001)

The mirror image duplication of denticle belts seen in abdominal segments 3, 5 and 7 of embryos expressing hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1 is rescued if they are also mutant for ci⁹⁴. The denticle types present (5 and 6) in tsh⁸ mutant embryos are unchanged if they are also expressing hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1, although in some segments the denticle belts are reduced and the shape of the denticle belt is modified.

(Gallet et al., 2000)

When expression is driven in a wg^l-17, wg^Scer\UAS.cLa, Scer\GAL4^en-e16E background the extent of naked cuticle anterior to the Scer\GAL4^en-e16E-driven wg source is narrower than in wg^l-17, wg^Scer\UAS.cLa, Scer\GAL4^en-e16E without the hh^Scer\UAS.cIa.

(Sanson et al., 1999)

The wing phenotype seen in flies expressing hh^Scer\UAS.cIa under the control of Scer\GAL4^en-e16E is completely suppressed by fu¹.

(Alves et al., 1998)

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1 in sgl⁰⁸³¹⁰ embryos induces naked cuticle at 16^oC and 25^oC.

(Hacker et al., 1997)

Scer\GAL4^e22c-mediated expression in ptc^G12 mutants changes the denticle belt morphology. Scer\GAL4^h-1J3-mediated expression in smo³ mutants at 18^oC does not modify the smo phenotype.

(Quirk et al., 1997)

Scer\GAL4^arm.PS mediated expression of both wg^Scer\UAS.cLa and hh^Scer\UAS.cIa in wg^l-17 Df(2R)en^E embryos causes naked cuticle to form in the place of the T1 beard.

(Lawrence et al., 1996)

Scer\GAL4^h-1J3 induced expression fails to rescue naked cuticle phenotype of smo^- embryos.

(van den Heuvel and Ingham, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

hh^UAS.cIa/Scer\GAL4^vg.PM rescues hh^ts2

(Vyas et al., 2008)

Partially rescues

hh^UAS.cIa/Scer\GAL4^69B partially rescues hh¹⁵/hh^AC

(Gallet et al., 2003)

Scer\GAL4^btl.PS/hh^UAS.cIa partially rescues hh⁸

(Glazer and Shilo, 2001)

Comments

Expression of hh^Scer\UAS.cIa under the control of Scer\GAL4^vg.PM completely rescues the lethality seen in homozygous hh^ts2 mutant flies grown at the restrictive temperature.

(Vyas et al., 2008)

Naked cuticle formation in hh¹⁵/hh^AC embryonic cuticle is rescued by hh^Scer\UAS.cIa; Scer\GAL4^69B, but the denticle belts formed consist of only row 1 2 and 3 type denticles.

(Gallet et al., 2003)

hh^Scer\UAS.cIa partially rescues the hh⁸ tracheal defects when expressed under the control of Scer\GAL4^btl.PS. In particular, rescue of the dorsal trunk, visceral branches and some lateral trunk anterior branches is seen. The ectodermal defects are not rescued in these embryos.

(Glazer and Shilo, 2001)

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Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

hh^Scer\UAS.cIa

hh^UAS.cIa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ingham

(Ingham and Fietz, 1995)

Secondary FlyBase IDs

References (48)

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