FlyBase Allele Report: Dmel\Hsp83[19F2]

General Information

Symbol

Dmel\Hsp83^19F2

Species

D. melanogaster

Name

FlyBase ID

FBal0049164

Feature type

allele

Associated gene

Dmel\Hsp83

Associated Insertion(s)

Carried in Construct

Also Known As

19F2

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Dickson et al., 1996)

Mutagen

ethyl methanesulfonate

(Dickson et al., 1996)

Progenitor genotype

Cytology

Description

Amino acid replacement: R48C.

(Rutherford and Lindquist, 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,194,640..3,194,640 [+]

Nucleotide change:

C3194640T

Amino acid change:

R48C | Hsp83-PA; R48C | Hsp83-PB

Reported amino acid change:

R48C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Rutherford and Lindquist, 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Dickson et al., 1996)

partially lethal (with Hsp83^6-55)

(Bandura et al., 2013)

viable (with Hsp83^e1D)

(Milton et al., 2005)

viable (with Hsp83^e3A)

(Milton et al., 2005)

Phenotype Manifest In

dorsal appendage (with Hsp83^e6A)

(Pisa et al., 2009)

egg (with Hsp83^e6A)

(Pisa et al., 2009)

egg chamber (with Hsp83^e6A)

(Pisa et al., 2009)

nurse cell (with Hsp83^e6A)

(Pisa et al., 2009)

Detailed Description

Statement

Reference

Hsp83^19F2/Hsp83^e6A females have a series of ovarian defects. Approximately 75% of egg chambers are blocked at different developmental stages, not later than stage 9. The remaining egg chambers show a pronounced defect in the transfer of nurse cell cytoplasm to the oocyte; the nuclei of the nurse cells do not degenerate at the end of the dumping process, as occurs in wild-type egg chambers, but persist up to later stages of oogenesis. Mutant mature eggs are smaller in size than normal and have altered dorsal appendages.

(Pisa et al., 2009)

Hsp83^19F2 has no effect on an invariant bristle trait (thoracic and scutellar bristles were analysed). Hsp83^19F2 has no significant effect on the trait mean of a variable bristle trait (the sternopleural, orbital, ocellar and vibrissa and carina bristles were analysed) compared to the background strain in which the Hsp83^19F2 mutation was induced.

(Milton et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Hsp83^19F2, Raf¹² has lethal | dominant phenotype

(Dickson et al., 1996)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsp83^19F2/Hsp83[+] is an enhancer of egg chamber phenotype of cup¹⁵

(Pisa et al., 2009)

Hsp83^19F2/Hsp83[+] is an enhancer of egg chamber phenotype of cup²¹

(Pisa et al., 2009)

Suppressor of

Statement

Reference

Hsp83^19F2/Hsp83[+] is a suppressor of arista | increased number phenotype of obk¹

(Atallah et al., 2004)

Hsp83^19F2 is a suppressor of eye phenotype of Raf::tor^12D.sev

(Dickson et al., 1996)

Other

Statement

Reference

Hsp83^19F2/Hsp83[+], cup²¹ has egg chamber phenotype

(Pisa et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The oogenesis defects seen in cup²¹ females are enhanced if they are also heterozygous for Hsp83^19F2; double mutants show earlier egg chamber growth arrest and precocious degeneration of the nurse cell nuclei. The double mutant egg chambers are often misshapen, with egg chambers either unbudded or fused (seen by the presence of supernumerary nurse cell nuclei in the egg chamber).

The oogenesis defects seen in cup¹⁵ females are enhanced if they are also heterozygous for Hsp83^19F2.

(Pisa et al., 2009)

Dominantly suppresses the rough eye phenotype of phl::tor^12D.sev. Synthetic lethal in combination with phl¹².

(Dickson et al., 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Hsp83^13F3 and Hsp83^19F2 are independent isolates of the same point mutation.

(Rutherford and Lindquist, 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

19F2

(Bandura et al., 2013, Rutherford and Lindquist, 1998)

Hsp83^19F2

(Bandura et al., 2013, Pisa et al., 2009)

Su(Raf)3A^19F2

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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