FlyBase Allele Report: Dmel\esg[05729]

General Information

Symbol

Dmel\esg⁰⁵⁷²⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0043049

Feature type

allele

Associated gene

Dmel\esg

Associated Insertion(s)

P{PZ}esg⁰⁵⁷²⁹

Carried in Construct

Also Known As

esg^P3

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

P-element activity

(Curtiss and Heilig, 1997, Fuse et al., 1994)

Delta2-3 transposase

(Hayashi et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{PZ}esg⁰⁵⁷²⁹

Cytology

Description

P{PZ} insertion 410bp upstream of the esg transcriptional start site.

(Chen et al., 2005)

P-element insertion within the esg promoter region.

(Fuse et al., 1994)

Insertion of P{lacZ}, close to transcription start site (S. Hayashi, unpublished observations).

(Hayashi et al., 1993)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division (with esg^35Ce-3)

(Hayashi et al., 1993)

lethal | recessive

(Roote, 1995.5.15, Hayashi et al., 1993)

lethal - all die during embryonic stage | recessive

(Fuse et al., 1994)

some die during embryonic stage | recessive

(Fuse et al., 1994)

Phenotype Manifest In

histoblast

(Hayashi, 1996)

nucleus (with esg^35Ce-3)

(Hayashi et al., 1993)

testis (with esg^35Ce-1)

(Streit et al., 2002)

wing (with esg^35Ce-3)

(Hayashi et al., 1993)

Detailed Description

Statement

Reference

esg⁰⁵⁷²⁹/esg^35Ce-1 pharate pupae have well-differentiated testes. The stem cells and spermatogenic cells that are normally found at the tip of the testis are missing in the mutants, with the mutant testes containing only sperm bundles and motile sperm.

(Streit et al., 2002)

In esg^35Ce-3/esg⁰⁵⁷²⁹ larvae, abdominal histoblasts become polyploid.

(Hayashi, 1996)

Heterozygotes with esg^35Ce-3 survive to the pharate adult stage, larval abdominal histoblasts undergo endoreplication and become polyploid preventing the development of the adult abdominal epidermis.

(Fuse et al., 1994)

Many heteroallelic combinations studied. Where flies die as pharate adults sternite/tergites are partially or completely deleted. Where flies are viable, they have held out wings, and may have sternite/tergites partially deleted and be sickly. Abdominal histoblasts were examined in heteroallelic combinations and found to have nuclei 3.4 times larger (revealed by Ecol\lacZ expression from esg⁰⁵⁷²⁹ allele) and with 7-16 times as much DNA (DAPI fluorescence) as wild type histoblast nuclei. esg⁰⁵⁷²⁹/esg^35Ce-3 larvae abnormally replicate the DNA of histoblasts (BrdU incorporation). Furthermore, haematoxylin staining revealed that proliferation of histoblasts does not occur during metamorphosis of esg⁰⁵⁷²⁹/esg^35Ce-3 flies. Humeral discs in esg⁰⁵⁷²⁹/esg^35Ce-3 flies have nuclei similar in size to wild type, and differentiate normal dorsal T1 structures.

(Hayashi et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

esg⁰⁵⁷²⁹/esg[+] is an enhancer of visible | dominant phenotype of L²

(Singh et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

esg⁰⁵⁷²⁹/esg[+] is an enhancer of eye phenotype of L²

(Singh et al., 2005)

Other

Statement

Reference

Cdk1^B47, esg⁰⁵⁷²⁹ has imaginal disc phenotype

(Hayashi, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

In cdc2^B47/+,esg^35Ce-3/esg⁰⁵⁷²⁹ imaginal discs are reduced in size and sometimes have cells with large nuclei.

(Hayashi, 1996)

Combination of phl mutation with esg⁰⁵⁷²⁹/esg^35Ce-3 greatly increased size of the imaginal cell nuclei.

(Hayashi et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

esg^k08104

(Prokopenko et al., 2000)

esg^k05907a

(BDGP Project Members, 1994-1999)

esg⁰⁷⁰⁸²

(BDGP Project Members, 1994-1999)

esg^k08104

(BDGP Project Members, 1994-1999)

esg^k02706

(BDGP Project Members, 1994-1999)

esg^k04802

(BDGP Project Members, 1994-1999)

esg^k01502

(BDGP Project Members, 1994-1999)

esg^k00606

(BDGP Project Members, 1994-1999)

Partially rescued by

esg⁰⁵⁷²⁹/esg^35Ce-3 is partially rescued by esg^hs.PF

(Fuse et al., 1994)

Comments

Introduction of esg^hs.PF and multiple heat shocks during embryonic and larval development significantly restores the amount of adult type abdominal cuticle.

(Fuse et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

83153

P{PZ}esg⁰⁵⁷²⁹/CyO

Notes on Origin

Discoverer

A. Spradling.

Spradling et al.

(Hayashi et al., 1993)

Putative cluster-mate of esg⁰⁵⁷³⁰.

(Ashburner et al., 1999)

Comments

Complements: CycE^k05002a. Complements: CycE^k02514.

(Prokopenko et al., 2000)

The Ecol\lacZ of this insertion is expressed identically to esg RNA of wild type, and was used to examine formation of imaginal tissues in an esg mutant background: no defects in formation of imaginal tissues were detectable.

(Hayashi et al., 1993)

Complements: wek^05271a.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

PZ05729

(Ashburner et al., 1999)

esg⁰⁵⁷²⁹

(Streit et al., 2002)

esg^P3

(Voog et al., 2014, Singh et al., 2005, Fung et al., 2002, Braun et al., 1997, Curtiss and Heilig, 1997, Yagi and Hayashi, 1997, Hayashi, 1996, Anonymous, 1993)

l(2)5729

l(2)esg⁰⁵⁷²⁹

(Roote, 1995.5.15)

l(2)esg^PZ10

(Roote, 1995.5.15)

Name Synonyms

Secondary FlyBase IDs

FBal0031218

References (18)

Report Sections

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