FB2024_03 , released June 25, 2024
Allele: Dmel\esg35Ce-3
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General Information
Symbol
Dmel\esg35Ce-3
Species
D. melanogaster
Name
FlyBase ID
FBal0003844
Feature type
allele
Associated gene
Dmel\esg
Associated Insertion(s)
Carried in Construct
Also Known As
esgVS8
Key Links
Genomic Maps

Allele class

loss of function allele

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Tanaka-Matakatsu et al., 1996)

loss of function allele

(Samakovlis et al., 1996)
Mutagen
ethyl methanesulfonate
(Ashburner et al., 1999.10.12, Samakovlis et al., 1996, Roote, 1995.5.4, Fuse et al., 1994)
Progenitor genotype
Cytology
Description

Amino acid change within the zinc finger: G387E and two other amino acid substitutions P149Q and M196I.

(Fuse et al., 1994)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:15,335,253..15,335,253 [+]
Nucleotide change:

G15335253A

Amino acid change:

G387E | esg-PA

Reported amino acid change:

G387E

Comment:

Amino acid change within the zinc finger: two other amino acid substitutions, P149Q and M196I, observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Fuse et al., 1994)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Heterozygous esg35Ce-3 mutant flies exhibit increased sensitivity to nicotine.

      (Sanchez-Díaz et al., 2015)

      In esg35Ce-3/esg05729 larvae, abdominal histoblasts become polyploid.

      (Hayashi, 1996)

      The dorsal branch fusion cells form discrete branches that never meet or join. These cells go on to form additional branches that resemble normal terminal branches in the larva. Lateral trunk fusion branches are completely missing leaving a gap in each segment of the lateral trunk.

      (Samakovlis et al., 1996)

      Heterozygotes with esg05729 survive to the pharate adult stage, larval abdominal histoblasts undergo endoreplication and become polyploid preventing the development of the adult abdominal epidermis.

      (Fuse et al., 1994)

      Many heteroallelic combinations studied. Where flies die as pharate adults sternite/tergites are partially or completely deleted and/or legs are twisted. Where flies are viable, they have held out wings, and may have sternite/tergites partially deleted and be sickly. Abdominal histoblasts were examined in heteroallelic combinations and found to have nuclei 3.4 fold times larger (revealed by Ecol\lacZ expression from esg05729 allele) and with 7-16 times as much DNA (DAPI fluorescence) as wild type histoblast nuclei. esg05729/esg35Ce-3 larvae abnormally replicate the DNA of histoblasts (BrdU incorporation). Furthermore, haematoxylin staining revealed that proliferation of histoblasts does not occur during metamorphosis of esg05729/esg35Ce-3 flies. Humeral discs in esg05729/esg35Ce-3 flies have nuclei similar in size to wild type, and differentiate normal dorsal T1 structures.

      (Hayashi et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Other
      Statement
      Reference

      Cdk1B47, esg35Ce-3 has imaginal disc phenotype

      (Hayashi, 1996)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In cdc2B47/+,esg35Ce-3/esg05729 imaginal discs are reduced in size and sometimes have cells with large nuclei.

      (Hayashi, 1996)

      Combination of phl mutation with esg05729/esg35Ce-3 greatly increased size of the imaginal cell nuclei.

      (Hayashi et al., 1993)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      esg05729/esg35Ce-3 is partially rescued by esghs.PF

      (Fuse et al., 1994)
      Comments

      Introduction of esghs.PF and multiple heat shocks during embryonic and larval development significantly restores the amount of adult type abdominal cuticle.

      (Fuse et al., 1994)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      Simpson.

      P. Simpson.

      (Roote, 1995.5.4)

      Induced on: l(2)CA61VS.

      (Roote, 1997.8.13)
      Comments
      Comments

      Strong esg allele.

      (Samakovlis et al., 1996, Fuse et al., 1994)

      Phenotypic data included in FBrf0051973.

      (Roote, 1995.5.4)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (12)