FlyBase Allele Report: Dmel\N[icd.hs]

Expression of N^icd.hs produces adult eyes containing ommatidia with 4-5 outer cells and up to three R7-like cells. Some ommatidia have only one or two R7 photoreceptors.

(Cooper and Bray, 2000)

37^oC heat pulse at 24hr APF in pupae being raised at 29^oC causes a mild double socket phenotype.

(Schweisguth, 1999)

The number of eve-expressing pericardial cells is increased and DA1 muscles usually fail to form in embryos in which N^icd.hs is expressed using heat shock at late stage 11. A similar phenotype is seen in these embryos are also expressing numb^{fl.Scer\UAS.T:Hsap\MYC} under the control of both Scer\GAL4^how-24B and Scer\GAL4^twi.PG.

(Park et al., 1998)

60 minute heat shock at 37^oX at 7-9 hours APF causes complete loss of microchaetes on the notum and shaft to socket transformation of macrochaetes. kuz^e29-4 mutant clones on the notum cause the formation of clusters of macrochaetes and microchaetes, microchaetae clusters are abolished by expression of N^icd.hs at 7-9 hours APF.

(Pan and Rubin, 1997)

Strong antineurogenic phenotype that is independent of the N background.

(Lieber et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

N^icd.hs has visible | heat sensitive phenotype, non-suppressible by ed^ΔECD.UAS/Scer\GAL4^sca-537.4

(Escudero et al., 2003)

Suppressor of

Statement

Reference

N^icd.hs is a suppressor of neurogenic phenotype | maternal effect | embryonic stage 8 phenotype of amx¹

(Michellod and Randsholt, 2008)

N^icd.hs is a suppressor of lethal | neurogenic phenotype | recessive | germline clone phenotype of Nct^agro

(Lopez-Schier and St. Johnston, 2002)

Other

Statement

Reference

N^icd.hs, amx¹/amx[+] has partially lethal - majority live phenotype

(Michellod and Randsholt, 2008)

N^icd.hs, amx¹/amx[+] has majority die during embryonic stage phenotype

(Michellod and Randsholt, 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^icd.hs has macrochaeta phenotype, enhanceable by Prosβ6¹

(Schweisguth, 1999)

N^icd.hs has tormogen cell | ectopic phenotype, enhanceable by Prosβ6¹

(Schweisguth, 1999)

N^icd.hs has macrochaeta phenotype, enhanceable by Prosβ2¹

(Schweisguth, 1999)

N^icd.hs has tormogen cell | ectopic phenotype, enhanceable by Prosβ2¹

(Schweisguth, 1999)

NOT suppressed by

Statement

Reference

N^icd.hs has microchaeta | heat sensitive phenotype, non-suppressible by ed^ΔECD.UAS/Scer\GAL4^sca-537.4

(Escudero et al., 2003)

Suppressor of

Statement

Reference

N^icd.hs is a suppressor of egg chamber phenotype of Mettl3^c1

(Hongay and Orr-Weaver, 2011)

N^icd.hs is a suppressor | heat sensitive of trichogen cell phenotype of Scer\GAL4^neur-P72, trbl^UAS.cMb

(Fichelson and Gho, 2004)

N^icd.hs is a suppressor of nervous system phenotype of bib^unspecified

(Lieber et al., 1993)

NOT Suppressor of

Statement

Reference

N^icd.hs is a non-suppressor of nervous system phenotype of E(spl)m8-HLH^unspecified

(Lieber et al., 1993)

Other

Statement

Reference

N^icd.hs, Scer\GAL4^neur-P72, trbl^UAS.cMb has external sensory organ precursor cell I phenotype

(Fichelson and Gho, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of N^icd.hs by performing daily heat-shocks for 5 days of adulthood significantly reduces the frequency of compound egg chamber formation in Ime4^c1 mutant ovaries and allows progression through oogenesis past the previtellogenic stages.

(Hongay and Orr-Weaver, 2011)

The heat-shocked progeny from heterozygous amx¹ females crossed to N^icd.hs males hatch at a reduced rate compared to wild type and only around 50% reach adulthood.

Fewer neurogenic phenotypes are seen in stage 8 embryos when homozygous amx¹ mutant females are crossed to N^icd.hs males (heat-shocked before the first delamination round) than when amx¹ females are crossed to wild type males.

(Michellod and Randsholt, 2008)

The loss of microchaetae seen in animals expressing N^icd.hs using heat shock in 0-8 hour old pupae is not rescued by expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-537.4.

(Escudero et al., 2003)

The N^icd.hs mild double socket phenotype is enhanced by Pros26¹ and Prosβ2¹. The N^icd.hs gene product is more stable in Pros26¹ expressing cells.

(Schweisguth, 1999)

Fails to rescue the neurogenic phenotype of E(spl) mutants but rescues the neurogenic phenotype of bib mutants.

(Lieber et al., 1993)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

N^icd.hs rescues N^264-47

(Lieber et al., 1993)

Comments

Able to rescue the cuticle phenotype of N^264-47 embryos.

(Lieber et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51661

w^*; P{hs-N.icd}2/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into S2 cells and used in biochemical assays.

(Wesley, 1999)

Incomplete N protein.

(Lieber et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

N^icd.hs

N^intra

(Remaud et al., 2008)

S2-N^-I

(Wesley, 1999)

S2-N^intra

(Wesley, 1999)

Name Synonyms

Nintra

(Schweisguth, 1999)

intracellular domain

(Lieber et al., 1993)

Secondary FlyBase IDs

References (21)

Report Sections

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