FlyBase Allele Report: Dmel\ed[ΔECD.UAS]

General Information

Symbol

Dmel\ed^ΔECD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0155345

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

Carried in Construct

P{UAS-ed.ΔECD}

Key Links

Transgenic product class

inframe_deletion

(Escudero et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Escudero et al., 2003)

Mutagen

in vitro construct

(Escudero et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-ed.ΔECD}

Cytology

Description

UAS regulatory sequences drive expression of the transmembrane domain and entire intracellular domain of ed.

(Escudero et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

visible, with Scer\GAL4^GMR.PF

(Escudero et al., 2003)

visible, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

visible, with Scer\GAL4^sca-C253

(Escudero et al., 2003)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Escudero et al., 2003)

macrochaeta, with Scer\GAL4^sca-C253

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^sca-537.4

(Escudero et al., 2003)

macrochaeta | ectopic, with Scer\GAL4^sca-C253

(Escudero et al., 2003)

ommatidium, with Scer\GAL4^GMR.PF

(Escudero et al., 2003)

wing vein | ectopic, with Scer\GAL4^ap-md544

(Escudero et al., 2003)

Detailed Description

Statement

Reference

The number of macrochaetae/heminotum in animals expressing ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^ap-md544 is - anterior+posterior notopleural: 2.68, presutural: 1.60, anterior supraalar: 1.48, posterior supraalar: 1.15, anterior postalar: 1.05, posterior postalar: 1.00, anterior+posterior dorsocentral: 3.60 and anterior+posterior scutellar: 3.48. The number of macrochaetae/heminotum in animals expressing ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-C253 is - anterior+posterior notopleural: 3.73, presutural: 0.97, anterior supraalar: 1.00, posterior supraalar: 1.07, anterior postalar: 1.00, posterior postalar: 0.92, anterior+posterior dorsocentral: 2.10 and anterior+posterior scutellar: 2.50. Expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-537.4 results in extra macrochaetae or even tufts of macrochaetae on the notum. Expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^ap-md544 results in flies with extra wing veins. Expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^ap-md544 results in flies a rough eye phenotype.

(Escudero et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ed^ΔECD.UAS, Scer\GAL4^sca-537.4 is an enhancer of visible phenotype of Delta^DN.UAS, Scer\GAL4^sca-537.4

(Escudero et al., 2003)

NOT Suppressor of

Statement

Reference

ed^ΔECD.UAS, Scer\GAL4^sca-C253 is a non-suppressor of visible phenotype of N^int.G.UAS, Scer\GAL4^sca-C253

(Escudero et al., 2003)

ed^ΔECD.UAS/Scer\GAL4^sca-537.4 is a non-suppressor of visible | heat sensitive phenotype of N^ECN.hs

(Escudero et al., 2003)

ed^ΔECD.UAS/Scer\GAL4^sca-537.4 is a non-suppressor of visible | heat sensitive phenotype of N^icd.hs

(Escudero et al., 2003)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has visible phenotype

(Escudero et al., 2003)

Delta^DN.UAS, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has visible phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has visible phenotype

(Escudero et al., 2003)

Raf^UAS.F179, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has visible phenotype

(Escudero et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

ed^ΔECD.UAS, Scer\GAL4^sca-537.4 is an enhancer of macrochaeta | ectopic phenotype of Delta^DN.UAS, Scer\GAL4^sca-537.4

(Escudero et al., 2003)

ed^ΔECD.UAS, Scer\GAL4^sca-537.4 is an enhancer of microchaeta | ectopic phenotype of Delta^DN.UAS, Scer\GAL4^sca-537.4

(Escudero et al., 2003)

NOT Suppressor of

Statement

Reference

ed^ΔECD.UAS, Scer\GAL4^sca-C253 is a non-suppressor of macrochaeta phenotype of N^int.G.UAS, Scer\GAL4^sca-C253

(Escudero et al., 2003)

ed^ΔECD.UAS/Scer\GAL4^sca-537.4 is a non-suppressor of microchaeta | heat sensitive phenotype of N^ECN.hs

(Escudero et al., 2003)

ed^ΔECD.UAS/Scer\GAL4^sca-537.4 is a non-suppressor of microchaeta | heat sensitive phenotype of N^icd.hs

(Escudero et al., 2003)

Other

Statement

Reference

Egfr^UAS.cBa, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has macrochaeta | ectopic phenotype

(Escudero et al., 2003)

Delta^DN.UAS, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has macrochaeta | ectopic phenotype

(Escudero et al., 2003)

Delta^DN.UAS, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has microchaeta | ectopic phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has macrochaeta phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has macrochaeta | ectopic phenotype

(Escudero et al., 2003)

N^ECN.UAS, Scer\GAL4^sca-C253, ed^ΔECD.UAS has microchaeta phenotype

(Escudero et al., 2003)

Raf^UAS.F179, Scer\GAL4^sca-537.4, ed^ΔECD.UAS has macrochaeta phenotype

(Escudero et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of N^ECN.Scer\UAS and ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-C253 results in loss of most microchaetae on the notum and either loss of macrochaetae or tufts of bristles in their place. Clusters of neurons appear at the sites of the developing sensory organs in pupal notum. The neurogenic phenotype caused by expression of Dl^D.Scer\UAS under the control of Scer\GAL4^sca-537.4 is potentiated by co-expression of ed^{ΔECD.Scer\UAS}, resulting in large tufts of macrochaetae and an increased density of microchaetae in the notum. The lack of bristles phenotype on the notum seen in flies expressing N^{int.G.Scer\UAS} under the control of Scer\GAL4^sca-C253 is not modified by co-expression of ed^{ΔECD.Scer\UAS}. The loss of microchaetae seen in animals expressing N^icd.hs using heat shock in 0-8 hour old pupae is not rescued by expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-537.4. The loss of microchaetae seen in animals expressing N^ECN.hs using heat shock is not rescued by expression of ed^{ΔECD.Scer\UAS} under the control of Scer\GAL4^sca-537.4. Co-expression of ed^{ΔECD.Scer\UAS} and Egfr^Scer\UAS.cBa under the control of Scer\GAL4^sca-537.4 results in severe tufting of bristles on the notum.

(Escudero et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ed^{ΔECD.Scer\UAS}

ed^ΔECD.UAS

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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