FlyBase Allele Report: Dmel\mRpL12[10534]

General Information

Symbol

Dmel\mRpL12¹⁰⁵³⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0028301

Feature type

allele

Associated gene

Dmel\mRpL12

Associated Insertion(s)

P{PZ}mRpL12¹⁰⁵³⁴

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}mRpL12¹⁰⁵³⁴

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,956,841..8,956,841 [+]

(Casad et al., 2011, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size (with Df(3L)Scf-R6)

(Frei et al., 2005)

decreased cell number | cell autonomous | somatic clone

(Frei et al., 2005)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage (with Df(3L)Scf-R6)

(Frei et al., 2005)

some die during larval stage (with Df(3L)Scf-R6)

(Frei et al., 2005)

Phenotype Manifest In

mitochondrion | somatic clone

(Frei et al., 2005)

Detailed Description

Statement

Reference

No cardiac or bristle phenotypes are observed in heterozygous mRpL12¹⁰⁵³⁴ mutant females.

(Casad et al., 2011)

Mutants do not display a dominant Minute bristle phenotype.

(Marygold et al., 2007)

mRpL12¹⁰⁵³⁴/Df(3L)Scf-R6 animals die during larval development, 70-80 h after egg deposition. Mutant larvae are thinner than controls, but do not exhibit a developmental delay as they enter second instar on time. When homozygous mutant clones are made in imaginal discs, the clones survive but have a growth disadvantage that led to smaller clones than controls. This does not seem to be due to an in increase in apoptosis, and cell size appears normal suggesting a slower growth rate. This effect is cell autonomous.

(Frei et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mRpL12¹⁰⁵³⁴ is a suppressor of increased cell size phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Frei et al., 2005)

mRpL12¹⁰⁵³⁴ is a suppressor of increased cell number | somatic clone phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^Act5C.PP

(Frei et al., 2005)

mRpL12¹⁰⁵³⁴ is a suppressor of abnormal cell growth | somatic clone phenotype of Hph^UAS.cFa, Scer\GAL4^Act5C.PP

(Frei et al., 2005)

Other

Statement

Reference

Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^Act5C.PP, mRpL12¹⁰⁵³⁴ has increased occurrence of cell division | somatic clone | larval stage phenotype

(Frei et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

mRpL12¹⁰⁵³⁴ is a suppressor of eye phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Frei et al., 2005)

mRpL12¹⁰⁵³⁴ is a suppressor of ommatidium phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Frei et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The eye phenotypes seen in CycD^Scer\UAS.cDa, Cdk4^Scer\UAS.cMa (driven by Scer\GAL4^GMR.PF) animals is suppressed by mRpL12¹⁰⁵³⁴. When mRpL12^{Scer\UAS.P\T.T:Avic\GFP-EGFP} is driven by Scer\GAL4^hs.PH and exposed to 3 1h heat shocks per day (37^oC) suppresses the larval lethality and the small size seen in mRpL12¹⁰⁵³⁴/Df(3L)Scf-R6 animals. Clones made in the fat body are also small and contain less DNA than control cells. A reduction is seen of mitochondria associated staining.