FlyBase Allele Report: Dmel\amos[Roi-1]

General Information

Symbol

Dmel\amos^Roi-1

Species

D. melanogaster

Name

FlyBase ID

FBal0014646

Feature type

allele

Associated gene

Dmel\amos

Associated Insertion(s)

Carried in Construct

Also Known As

Roi, Roi¹

Key Links

Allele class

gain of function allele

Mutagen

spontaneous

Nature of the Allele

Allele class

gain of function allele

(Heberlein et al., 1993)

Mutagen

spontaneous

(Lindsley and Zimm, 1992)

Progenitor genotype

Caused by aberration

In(2L)Roi¹

Cytology

Description

Inversion between 36A and 37A. The proximal breakpoint is 2.6kb downstream of amos.

(Chanut et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Lindsley and Zimm, 1992, Renfranz and Benzer, 1989)

viable

(Lindsley and Zimm, 1992)

visible

(Renfranz and Benzer, 1989)

Phenotype Manifest In

Detailed Description

Statement

Reference

The ommatidia of amos^Roi-1 mutant flies have variable numbers of cone cells (1-6 per ommatidium). The shapes and patterns of packing of these cone cells corresponds to the patterns of packing seen in aggregates of identically sized soap bubbles: a single cone cell is round; clusters of 2-5 cone cells all pack in the pattern primarily observed for a soap bubble aggregate of that number; clusters of 6 cone cells pack in one of the three stable configuration seen for soap bubble aggregates of that number.

(Hayashi and Carthew, 2004)

Heterozygotes show a severe disruption of the ommatidial lattice in the retina. The ommatidia can contain either more or less than the normal number of photoreceptor cells (for example the small rhabdomeres characteristic of photoreceptor cells R7 and R8 can either be missing or clustered). Pigment cells are often missing, resulting in large clusters of photoreceptors that include the equivalent of 2 to 3 normal ommatidia. R8 precursors are irregularly spaced and show occasional twinning in third larval instar eye discs, and there is disorganised recruitment of other ommatidial cells. Homozygous clones in the retina are smaller than their wild-type twin spots and contain very aberrant photoreceptors with fused or distorted rhabdomeres. Wild-type clones in the retina in a heterozygous background develop into a regular array of normal ommatidia, while the surrounding heterozygous tissue develops into abnormal ommatidia. At the edge of the clone, mosaic ommatidia (containing both wild type and heterozygous photoreceptors) are usually abnormal in structure.

(Chanut et al., 2002)

Eye facets of amos^Roi-1/+ irregularly rounded, sometimes enlarged; eyes sometimes bulge. RK2A.

(Lindsley and Zimm, 1992)

Adult eyes are rough with irregular round bulging facets. Variable number of rhabdomeres per ommatidia. Highly variable size and shape of clusters on eye disc.

(Renfranz and Benzer, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

amos^Roi-1/amos[+] is a suppressor of visible | dominant phenotype of ro^DOM

(Chanut et al., 2002)

amos^Roi-1/amos[+] is a suppressor of visible | recessive phenotype of ato¹

(Chanut et al., 2002)

amos^Roi-1/amos[+] is a suppressor of visible phenotype of ro^DOM

(Heberlein et al., 1993)

amos^Roi-1/amos[+] is a suppressor of visible phenotype of ro^hs.PI

(Heberlein et al., 1993)

Other

Statement

Reference

amos^Roi-1, smi26D¹/smi26D[+] has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

smi21F[+]/Pino^smi21F, amos^Roi-1 has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

Pino^smi21F, amos^Roi-1 has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

amos^Roi-1, smi26D¹ has abnormal smell perception | dominant | female phenotype

(Anholt et al., 2003)

S¹, amos^Roi-1 has viable phenotype

(Lindsley and Zimm, 1992)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

amos^Roi-1 has ommatidium phenotype, non-enhanceable by ato¹/ato[+]

(Chanut et al., 2002)

Suppressed by

Statement

Reference

amos^Roi-1 has ommatidium phenotype, suppressible by hh[+]/hh⁸

(Chanut et al., 2002)

amos^Roi-1 has ommatidium phenotype, suppressible by da[+]/da¹⁰

(Chanut et al., 2002)

NOT suppressed by

Statement

Reference

amos^Roi-1 has ommatidium phenotype, non-suppressible by ato¹/ato[+]

(Chanut et al., 2002)

amos^Roi-1 has ommatidium phenotype, non-suppressible by dac^unspecified

(Chanut et al., 2002)

Enhancer of

Statement

Reference

amos^Roi-1/amos[+] is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, fru^NP0021

(Goto et al., 2011)

amos^Roi-1 is an enhancer of eye phenotype of Scer\GAL4^hs.2sev, mbl^C.UAS

(Vicente-Crespo et al., 2008)

Suppressor of

Statement

Reference

amos^Roi-1/amos[+] is a suppressor of eye phenotype of ro^DOM

(Chanut et al., 2002, Heberlein et al., 1993)

amos^Roi-1/amos[+] is a suppressor of eye phenotype of ato¹

(Chanut et al., 2002)

amos^Roi-1 is a suppressor | partially of phenotype of Dr^Mio

(Heberlein et al., 1993)

amos^Roi-1/amos[+] is a suppressor of eye phenotype of ro^hs.PI

(Heberlein et al., 1993)

amos^Roi-1 is a suppressor of phenotype of hh^bar3

(Heberlein et al., 1993)

Additional Comments

Genetic Interactions

Statement

Reference

An amos^Roi-1 background enhances the rough eye phenotype found upon expression of fru^NP0021 under the control of Scer\GAL4^GMR.PF.

(Goto et al., 2011)

The eyes of amos^Roi-1/+ ; ato¹/ato¹ flies can reach up to 1/3 of wild-type size. The ommatidial clusters appear disorganised and contain an abnormal number of photoreceptors, whose rhabdomeres are often elongated and misshapen. R8 cells appear to be present in most if not all ommatidia. hh⁸ dominantly suppresses the amos^Roi-1/+ phenotype; at least 50% of the ommatidia have normal structure and orientation in double heterozygotes. da¹⁰/+ almost completely suppresses the amos^Roi-1/+ retina phenotype. amos^Roi-1 complements lethal dac mutant alleles and the amos^Roi-1 rough eye phenotype is insensitive to a reduction in the dosage of the dac gene.