FlyBase Allele Report: Dmel\Pp1-87B[87Bg-6]

General Information

Symbol

Dmel\Pp1-87B^87Bg-6

Species

D. melanogaster

Name

FlyBase ID

FBal0013941

Feature type

allele

Associated gene

Dmel\Pp1-87B

Associated Insertion(s)

Carried in Construct

Also Known As

PP1α87B^87Bg-6, ck19^hs46

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Axton et al., 1990)

Mutagen

ethyl methanesulfonate

(Baksa et al., 1993)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Axton et al., 1990)

Description

Pp1-87B^87Bg-6 is deficient for approximately 500bp of the 5' end of the Pp1-87B gene, consequently it lacks the translation start site.

(Axton et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage

(Axton et al., 1990)

abnormal size | third instar larval stage

(Axton et al., 1990)

increased occurrence of cell division | third instar larval stage

(Axton et al., 1990)

lethal | recessive

(Axton et al., 1990)

Phenotype Manifest In

embryonic/larval optic lobe (with Pp1-87B^87Bg-1)

(Babu et al., 2005)

embryonic/larval optic lobe (with Pp1-87B^87Bg-3)

(Babu et al., 2005)

ganglion | third instar larval stage

(Axton et al., 1990)

ganglion mother cell

(Baksa et al., 1993)

imaginal disc

(Axton et al., 1990)

mitotic spindle | third instar larval stage

(Axton et al., 1990)

neuroblast

(Baksa et al., 1993)

photoreceptor cell R1 & axon (with Pp1-87B^87Bg-1)

(Babu et al., 2005)

photoreceptor cell R1 & axon (with Pp1-87B^87Bg-3)

(Babu et al., 2005)

photoreceptor cell R2 & axon (with Pp1-87B^87Bg-1)

(Babu et al., 2005)

photoreceptor cell R2 & axon (with Pp1-87B^87Bg-3)

(Babu et al., 2005)

photoreceptor cell R3 & axon (with Pp1-87B^87Bg-1)

(Babu et al., 2005)

photoreceptor cell R3 & axon (with Pp1-87B^87Bg-3)

(Babu et al., 2005)

photoreceptor cell R4 & axon (with Pp1-87B^87Bg-1)

(Babu et al., 2005)

photoreceptor cell R4 & axon (with Pp1-87B^87Bg-3)

(Babu et al., 2005)

photoreceptor cell R5 & axon (with Pp1-87B^87Bg-1)

(Babu et al., 2005)

photoreceptor cell R5 & axon (with Pp1-87B^87Bg-3)

(Babu et al., 2005)

Detailed Description

Statement

Reference

Both Pp1-87B^87Bg-1/Pp1-87B^87Bg-6 and Pp1-87B^87Bg-3/Pp1-87B^87Bg-6 larvae show axon clumping at the lamina and laminal breaks with ~50% of R2-R5 axons being mistargeted to the medulla.

(Babu et al., 2005)

Weak suppressor levels of position effect vareigation and a high frequency of aberrant mitosis in neuroblasts or ganglion mother cells. Metaphase chromosomes and anaphase chromatids are over condensed.

(Baksa et al., 1993)

Larvae die either just before or just after pupariation. Third instar larval imaginal discs are underdeveloped, indicating failure of diploid cell proliferation. Ganglia are smaller than those of wild-type larvae, with significantly fewer cells per microscope field in squash preparations, suggesting cell division is impaired in the ganglia. The proportion of cells in mitosis in the ganglia is significantly higher than in wild type. This is due to a large number of mitotic cells showing abnormalities, such as overcondensed or extra chromosomes. These cells are probably delayed in progress through mitosis. Spindle organisation is also defective, showing unusually little space between almost parallel bundles of spindle microtubules at metaphase.

(Axton et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pp1-87B[+]/Pp1-87B^87Bg-6 is an enhancer of visible phenotype of Scer\GAL4^vg.PM, put^UAS.cMa

(Bennett et al., 2003)

Suppressor of

Statement

Reference

Pp1-87B[+]/Pp1-87B^87Bg-6 is a suppressor | partially of visible | homeotic phenotype of trx^E2/trx¹

(Rudenko et al., 2003)

Pp1-87B[+]/Pp1-87B^87Bg-6 is a suppressor | partially of visible | homeotic phenotype of trx^B11/trx¹

(Rudenko et al., 2003)

Other

Statement

Reference

NiPp1^UAS.EGFP, Pp1-87B^87Bg-6, Scer\GAL4^vg.PM has visible phenotype

(Parker et al., 2002)

Phenotype Manifest In

Enhancer of

Statement

Reference

Pp1-87B[+]/Pp1-87B^87Bg-6 is an enhancer of wing phenotype of Scer\GAL4^vg.PM, put^UAS.cMa

(Bennett et al., 2003)

Pp1-87B[+]/Pp1-87B^87Bg-6 is an enhancer of wing blade posterior compartment phenotype of Scer\GAL4^vg.int2.1, put^UAS.cMa

(Bennett and Alphey, 2002)

Suppressor of

Statement

Reference

Pp1-87B[+]/Pp1-87B^87Bg-6 is a suppressor | partially of wing phenotype of trx^E2/trx¹

(Rudenko et al., 2003)

Pp1-87B[+]/Pp1-87B^87Bg-6 is a suppressor | partially of haltere | ectopic phenotype of trx^E2/trx¹

(Rudenko et al., 2003)

Pp1-87B[+]/Pp1-87B^87Bg-6 is a suppressor | partially of wing phenotype of trx^B11/trx¹

(Rudenko et al., 2003)

Pp1-87B[+]/Pp1-87B^87Bg-6 is a suppressor | partially of haltere | ectopic phenotype of trx^B11/trx¹

(Rudenko et al., 2003)

Pp1-87B^87Bg-6 is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

Pp1-87B^87Bg-6 is a suppressor of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Pp1-87B^87Bg-6 is a suppressor of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

Other

Statement

Reference

NiPp1^UAS.EGFP, Pp1-87B^87Bg-6, Scer\GAL4^vg.PM has wing phenotype

(Parker et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The modest wing overgrowth phenotype caused by expression of put^Scer\UAS.cMa under the control of Scer\GAL4^vg.PM is enhanced by Pp1-87B^87Bg-6/+.

(Bennett et al., 2003)

Pp1-87B^87Bg-6 dominantly suppresses the transformation of wing to haltere seen in trx¹/trx^E2 flies; 36% show strong transformation, 42% show weak transformation and 22% show no transformation.

Pp1-87B^87Bg-6 dominantly suppresses the transformation of wing to haltere seen in trx¹/trx^B11 flies; 28% show strong transformation, 29% show weak transformation and 43% show no transformation.

(Rudenko et al., 2003)

Flies expressing NiPp1^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^vg.PM which are also carrying Pp1-87B^87Bg-6 have crumpled wings.

(Parker et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pp1-87B^87Bg-6 is rescued by Pp1-87B^+t5.8

(Baksa et al., 1993)

Comments

The lethality of Pp1-87B^87Bg-6 is rescued by Pp1-87B^+t5.8.

(Baksa et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

PP1α87B^87Bg-6

(Babu et al., 2005, Bennett et al., 2003, Rudenko et al., 2003, Parker et al., 2002)

Pp1-87B^87Bg-6

Pp1-87B^Bg6

(Roubinet et al., 2017)

Pp1-87B^hs46

(Bennett and Alphey, 2002)

ck19^hs46

(Axton et al., 1990, Dombradi et al., 1990)

hs46

(Baksa et al., 1993)

hs⁴⁶

(Gausz et al., 1981)

l(3)87Bg⁶

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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