FlyBase Allele Report: Dmel\ort[4]

General Information

Symbol

Dmel\ort⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0013287

Feature type

allele

Associated gene

Dmel\ort

Associated Insertion(s)

Carried in Construct

Also Known As

ort^US2515

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

No point mutation is detected in the open reading frame.

(Gengs et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology

(Rajaram et al., 2005)

abnormal optomotor response (with ort¹)

(Rister et al., 2007)

abnormal phototaxis (with ort¹)

(Gao et al., 2008)

abnormal phototaxis (with ort⁶)

(Gao et al., 2008)

chemical sensitive | adult stage

(Georgiev et al., 2002)

chemical sensitive | adult stage (with Df(3R)12)

(Georgiev et al., 2002)

chemical sensitive | larval stage

(Georgiev et al., 2002)

Phenotype Manifest In

rhabdomere

(O'Tousa et al., 1989)

Detailed Description

Statement

Reference

Compared to controls, ort¹/ort⁴ mutants exhibit weaker phototaxis towards either UV or green light, with UV phototaxis being affected the most severely in an intensity-dependent manner. ort⁴/ort⁶ mutants exhibit weaker phototaxis towards UV light.

(Gao et al., 2008)

Flies combining ort¹ with ort⁴ are largely, but not entirely, motion-blind, giving occasional small responses in head yaw and roll optomotor assays, but are entirely motion-blind when tested for optomotor yaw torque (with a fixed head position) and under low pattern contrast conditions.

Heteroallelic mutants ort¹/ort⁴ do not exhibit optomotor responses during tethered flight in low light intensities.These mutants do not respond to front-to-back or back-to-front motion at pattern contrasts of 5%, 10% or 40%.

(Rister et al., 2007)

After a 3-second light pulse, the return to baseline in the extracellular potentials measured at the surface of the eye tends to be slower in ort⁴ mutants compared to controls. Immediately after lights-off, both the wild-type and mutant electroretinograms start a sharp recovery, but soon the mutant lags behind to wild-type. This phenotype is much diminished after a 0.2-second pulse of light compared to a 3-second pulse. Indeed, in the majority of cases, the individual mutant flies that display slow termination kinetics after a long pulse have a faster repolarization after a short pulse.

(Rajaram et al., 2005)

Mutant larvae and adults show increased susceptibility to abamectin or ivermectin compared to wild type (viability is reduced). They do not show any increased sensitivity to ethanol compared to wild type. ort⁴/Df(3R)12 adults show increased susceptibility to abamectin compared to wild type (viability is reduced). Mutant adults do not show increased sensitivity to dieldrin compared to wild type.

(Georgiev et al., 2002)

Rhabdomeres of young flies are reduced in size and show aberrant packing of microvilli. Rhabdomeres are nearly wild type in adults.

(O'Tousa et al., 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

HisCl1¹³⁴, ninaE¹, ort⁴/ort¹ has abnormal phototaxis phenotype, enhanceable by ort^{UAS.2x.Tag:HA}/Scer\GAL4^DBD.C1a.ort/Hsim\VP16^{AD-VGlut-OK371}

(Karuppudurai et al., 2014)

Other

Statement

Reference

HisCl1¹³⁴, ninaE¹, ort⁴/ort¹ has abnormal phototaxis phenotype

(Karuppudurai et al., 2014)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

ort¹/ort⁴ in a HisCl1¹³⁴, ninaE¹ mutant background significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.

Expressing ort^{Scer\UAS.2x.T:Ivir\HA1} in Tm5c neurons in a homozygous ort¹, ort¹/ort⁴, ort¹/ort⁶ background (with HisCl1¹³⁴ and ninaE¹) under the control of Scer\GAL4^{C1a.DBD.ort.T:Zzzz\ZipRREEL} and Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\ZipEERRL,T:SV40\nls2} enhances the preference for green light over UV seen in the mutant flies alone.

(Karuppudurai et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ort⁴/ort¹ is rescued by Scer\GAL4^ort.C1-4/ort^UAS.cWa

(Gao et al., 2008)

ort⁴/ort¹ is rescued by Hsim\VP16^{AD-VGlut-OK371}/ort^UAS.cWa/Scer\GAL4^DBD.ort.C1-3

(Gao et al., 2008)

ort⁴/ort¹ is rescued by Scer\GAL4^ort.C2/ort^UAS.cWa

(Gao et al., 2008)

ort⁴/ort¹ is rescued by Scer\GAL4^NP0723/ort^UAS.cWa

(Rister et al., 2007)

ort⁴/ort¹ is rescued by Scer\GAL4^NP6298/ort^UAS.cWa

(Rister et al., 2007)

Partially rescued by

ort⁴/ort¹ is partially rescued by Scer\GAL4^NP6298/ort^UAS.cWa

(Gao et al., 2008)

ort⁴/ort¹ is partially rescued by Scer\GAL4^c202a/ort^UAS.cWa

(Rister et al., 2007)

ort⁴/ort¹ is partially rescued by Scer\GAL4^21D/ort^UAS.cWa

(Rister et al., 2007)

Not rescued by

ort⁴/ort¹ is not rescued by Scer\GAL4^DBD.ChAT/Scer\GAL4^{AD.ort.C1-3.Tag:NLS(SV40-largeT)}/ort^UAS.cWa

(Gao et al., 2008)

Comments

Driving ort^Scer\UAS.cWa with Scer\GAL4^NP6298 in the ort¹/ort⁴ mutant background restores fly head yaw and roll optomotor responses to wildtype levels.

Expressing ort^Scer\UAS.cWa with either Scer\GAL4^NP0723 or Scer\GAL4^c202a in the ort¹/ort⁴ mutant background restores optomotor responses to wildtype levels measured by fly head yaw and roll optomotor responses.

Expression of ort^Scer\UAS.cWa with Scer\GAL4^NP6298 in the ort¹/ort⁴ mutant background rescues optomotor yaw responses at low pattern contrast of 0.1%, but not when driven with either Scer\GAL4^c202a or Scer\GAL4^21D. With a pattern contrast of 1%, ort¹/ort⁴ mutants driving ort^Scer\UAS.cWa with Scer\GAL4^21D show optomotor responses only slightly reduced compared to controls, but are statistically indistinguishable from negative controls when driving with Scer\GAL4^c202a.

Overexpression of ort^Scer\UAS.cWa with Scer\GAL4^21D in the ort¹/ort⁴ mutant background rescues response to low level light intensity defects of the mutants alone, but not when expressed by Scer\GAL4^c202a. However, Scer\GAL4^c202a rescues this phenotype when the pattern wavelength is doubled from lambda = 18[o] to 36[o].

Expression of ort^Scer\UAS.cWa with Scer\GAL4^21D in the ort¹/ort⁴ mutant background rescues response to front-to-back motion, but not when driven by Scer\GAL4^c202a, with a pattern contrast of 5%. Rescue of back-to-front motion defects occurs when expressing with Scer\GAL4^c202a at >5% pattern contrast, whilst Scer\GAL4^21D only rescues when the pattern contrast is 40%.

(Rister et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Hardy, Orevi and Merriam.

(Lindsley and Zimm, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ort⁴

ort^US2515

(Karuppudurai et al., 2014, Gao et al., 2008, Gao et al., 2008, Rister et al., 2007, Rajaram et al., 2005, Gengs et al., 2002, Georgiev et al., 2002, O'Tousa et al., 1989)

ort^us2515

(Schnaitmann et al., 2018)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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