FB2024_03 , released June 25, 2024
Allele: Dmel\ortUAS.cWa
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General Information
Symbol
Dmel\ortUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of White
FlyBase ID
FBal0243314
Feature type
allele
Associated gene
Dmel\ort
Associated Insertion(s)
Carried in Construct
P{UAS-ort.W}
Also Known As
UAS-ort
Key Links
Transgenic product class
wild_type
(Rister et al., 2007)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Rister et al., 2007)
Mutagen
in vitro construct
(Rister et al., 2007)
Progenitor genotype
Carried in construct
P{UAS-ort.W}
Cytology
Description

Expression of ort coding region is governed by UASt regulatory sequences.

(Rister et al., 2007)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
ort
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4ort.C1-4/ortUAS.cWa rescues ort4/ort1

      (Gao et al., 2008)

      Hsim\VP16AD-VGlut-OK371, ortUAS.cWa, Scer\GAL4DBD.ort.C1-3 rescues ort4/ort1

      (Gao et al., 2008)

      Scer\GAL4ort.C2/ortUAS.cWa rescues ort4/ort1

      (Gao et al., 2008)

      Scer\GAL4NP0723/ortUAS.cWa rescues ort4/ort1

      (Rister et al., 2007)

      Scer\GAL4NP6298/ortUAS.cWa rescues ort4/ort1

      (Rister et al., 2007)
      Partially rescues

      Scer\GAL4NP6298/ortUAS.cWa partially rescues ort4/ort1

      (Gao et al., 2008)

      Scer\GAL4c202a/ortUAS.cWa partially rescues ort4/ort1

      (Rister et al., 2007)

      Scer\GAL421D/ortUAS.cWa partially rescues ort4/ort1

      (Rister et al., 2007)
      Fails to rescue

      Scer\GAL4DBD.ChAT, Scer\GAL4AD.ort.C1-3.Tag:NLS(SV40-largeT), ortUAS.cWa fails to rescue ort4/ort1

      (Gao et al., 2008)
      Comments

      Overexpression of ortScer\UAS.cRa by Scer\GAL4ort.C1-4 restores the preference of ort1/ort4 mutants for UV light over green light to wildtype levels.

      Overexpression of ortScer\UAS.cRa with Scer\GAL4NP6298 qualitatively restores ERG and optomotor defects, but not UV preference defects, in ort1/ort4 mutant flies.

      Overexpression of ortScer\UAS.cRa with combinatorial driving by Scer\GAL4DBD.ort.C1-3.T:Zzzz\ZipRREEL and Hsim\VP16VGlut-OK371-dVP16AD rescues UV preference defects in ort1/ort4 mutants. In contrast, expression in cholinergic neurons combinatorially using Scer\GAL4AD.ort.C1-3.T:Zzzz\ZipEERRL,T:SV40\nls2 and Scer\GAL4DBD.Cha.T:Zzzz\ZipRREEL in the mutant background either reduces UV attraction or enhances attraction to green light.

      Expressing ortScer\UAS.cRa with Scer\GAL4ort.C2 in the ort1/ort4 mutant background rescues UV preference to wildtype levels.

      (Gao et al., 2008)

      Driving ortScer\UAS.cWa with Scer\GAL4NP6298 in the ort1/ort4 mutant background restores fly head yaw and roll optomotor responses to wildtype levels.

      Expressing ortScer\UAS.cWa with either Scer\GAL4NP0723 or Scer\GAL4c202a in the ort1/ort4 mutant background restores optomotor responses to wildtype levels measured by fly head yaw and roll optomotor responses.

      Expression of ortScer\UAS.cWa with Scer\GAL4NP6298 in the ort1/ort4 mutant background rescues optomotor yaw responses at low pattern contrast of 0.1%, but not when driven with either Scer\GAL4c202a or Scer\GAL421D. With a pattern contrast of 1%, ort1/ort4 mutants driving ortScer\UAS.cWa with Scer\GAL421D show optomotor responses only slightly reduced compared to controls, but are statistically indistinguishable from negative controls when driving with Scer\GAL4c202a.

      Overexpression of ortScer\UAS.cWa with Scer\GAL421D in the ort1/ort4 mutant background rescues response to low level light intensity defects of the mutants alone, but not when expressed by Scer\GAL4c202a. However, Scer\GAL4c202a rescues this phenotype when the pattern wavelength is doubled from lambda = 18[o] to 36[o].

      Expression of ortScer\UAS.cWa with Scer\GAL421D in the ort1/ort4 mutant background rescues response to front-to-back motion, but not when driven by Scer\GAL4c202a, with a pattern contrast of 5%. Rescue of back-to-front motion defects occurs when expressing with Scer\GAL4c202a at >5% pattern contrast, whilst Scer\GAL421D only rescues when the pattern contrast is 40%.

      (Rister et al., 2007)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      ortScer\UAS.cRa
      ortScer\UAS.cWa
      ortUAS.cWa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of White
      Secondary FlyBase IDs
        References (9)