FB2024_03 , released June 25, 2024
Allele: Dmel\HisCl1134
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General Information
Symbol
Dmel\HisCl1134
Species
D. melanogaster
Name
FlyBase ID
FBal0243129
Feature type
allele
Associated gene
Dmel\HisCl1
Associated Insertion(s)
Carried in Construct
Also Known As
hiscl134
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Delta2-3 transposase
(Hong et al., 2006)
Progenitor genotype
HisCl1EP73547
(Hong et al., 2006)
Cytology
Description

Imprecise excision of P{EP}HisCl1EP73547 has produced a 1.7kb deletion removing -25 to +1691 of HisCl1.

(Hong et al., 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3R:11,787,151..11,788,867 [+]
Comment:

A deletion resulting from the imprecise excision of P{EP}HisCl1EP73547 that removes sequences from -25 to +1691 relative to HisCl1.

(Hong et al., 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      As in wild type, robust hyperpolarizing "on" and depolarizing "off" transients are invariably seen in the electroretinograms of HisCl1134 mutant flies. The "off" transients are very similar to wild type, but although the "on" transients appear qualitatively similar to wild type, the amplitude is approximately two fold higher.

      Intracellular recordings from HisCl1134 mutant large monopolar cells (LMCs) have a similar resting potential to wild type and produce similar graded transient hyperpolarizations in response to 10ms bright flashes. The maximum responses are of a similar amplitude, but the rise time of the response waveforms is significantly slower than in wild type cells, with the time to peak delayed by ~10-20ms. The responses are also slightly less sensitive to low intensity flashes.

      (Pantazis et al., 2008)

      The temperature preference profile of HisCl1134 homozygous adults is abnormal with a significantly increased preference for higher temperatures compared to wild-type. Locomotor activity levels in these animals appear to be normal. However, when placed at 40'C, these flies are rendered unconscious at a significantly faster rate than wild-type and their recovery from a 10 minute 40'C heat shock is significantly slower than wild-type.

      (Hong et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      ort1 in a HisCl1134, ninaE1 mutant background significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.

      ort1/ort4 in a HisCl1134, ninaE1 mutant background significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.

      ort1/ort6 in a HisCl1134, ninaE1 mutant background significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.

      Expressing ortScer\UAS.2x.T:Ivir\HA1 in Tm5c neurons in a homozygous ort1, ort1/ort4, ort1/ort6 background (with HisCl1134 and ninaE1) under the control of Scer\GAL4C1a.DBD.ort.T:Zzzz\ZipRREEL and Hsim\VP16AD.VGlut.OK371.T:Zzzz\ZipEERRL,T:SV40\nls2 enhances the preference for green light over UV seen in the mutant flies alone.

      (Karuppudurai et al., 2014)

      HisCl1134 ort1 double mutants fail to discriminate between wavelengths in the UV and green spectrum, though are not entirely blind.

      HisCl1134,ort1/HisCl1134,ort6 double mutants show weak phototaxis towards green light.

      (Gao et al., 2008)

      The temperature preference profile of HisCl1134, ort1 homozygous adults is abnormal. There is a broader spread of temperature preferences with a significantly with fewer flies choosing the wild-type majority preference of 24-25'C. Locomotor activity levels in these animals appear to be normal. However, when placed at 40'C, these flies are rendered unconscious at a significantly faster rate than wild-type. However, when placed at 40'C, these flies are rendered unconscious at a significantly faster rate than wild-type and their recovery from a 10 minute 40'C heat shock is significantly slower than wild-type. These two phenotypes (reduced tolerance for high temperatures and slower recovery following heat shock) are both largely rescued by HisCl1+t14.3; ort+t13.3.

      HisCl1134, ort1 homozygous adults have a significantly increased death rate after 6 days at 4'C compared to wild-type, but they recover more quickly from a cold induced coma. These phenotypes are largely rescued by HisCl1+t14.3; ort+t13.3.

      ortScer\UAS.cHa; Scer\GAL4ort.PH suppresses the abnormal temperature response profile of HisCl1134 homozygotes.

      (Hong et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (11)