Deletion of 8 nucleotides (-276 to -269 relative to the proposed translation start site).
8 bp deletion in the 5'UTR of the ort transcript.
The temperature preference profile of ort6 homozygous adults is abnormal. There is a broader spread of temperature preferences with a significantly with fewer flies choosing the wild-type majority preference of 24-25'C and an increased preference for lower temperatures. Locomotor activity levels in these animals appear to be normal.
Mutants lack the on/off transients of the electroretinogram. Laminar L1/L2 neurons still show robust histamine-activated currents, but much higher concentrations of histamine are required, with the EC500 shifted to approximately 10 fold higher concentrations than normal.
HisCl1134, ninaE1, ort6/ort1 has abnormal phototaxis phenotype, enhanceable by ortUAS.2x.Tag:HA/Scer\GAL4DBD.C1a.ort/Hsim\VP16AD-VGlut-OK371
HisCl1134, ninaE1, ort6/ort1 has abnormal phototaxis phenotype
HisCl1134, ort6/ort1 has abnormal phototaxis phenotype
ort1/ort6 in a HisCl1134, ninaE1 mutant background significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.
Expressing ortScer\UAS.2x.T:Ivir\HA1 in Tm5c neurons in a homozygous ort1, ort1/ort4, ort1/ort6 background (with HisCl1134 and ninaE1) under the control of Scer\GAL4C1a.DBD.ort.T:Zzzz\ZipRREEL and Hsim\VP16AD.VGlut.OK371.T:Zzzz\ZipEERRL,T:SV40\nls2 enhances the preference for green light over UV seen in the mutant flies alone.
Pak.