FlyBase Allele Report: Dmel\mei-9[a]

General Information

Symbol

Dmel\mei-9^a

Species

D. melanogaster

Name

FlyBase ID

FBal0012171

Feature type

allele

Associated gene

Dmel\mei-9

Associated Insertion(s)

Carried in Construct

Also Known As

mei-9a, A

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yamamoto and Wada, 1990)

Progenitor genotype

Cytology

Description

Amino acid replacement: D658N. Nucleotide substitution: G2289A.

(Yildiz et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,315,637..4,315,637 [-]

Nucleotide change:

G4315637A

Reported nucleotide change:

G2289A

Amino acid change:

D701N | mei-9-PA; D701N | mei-9-PB

Reported amino acid change:

D658N

Comment:

Position of mutation on reference sequence inferred by FlyBase curator. Authors protein sequence lacks 43 N-terminal amino acid residues.

(Yildiz et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chamber

(Sarantseva and Khromykh, 2001)

mitosis & nuclear chromosome

(Gatti, 1979)

presumptive oocyte

(Joyce and McKim, 2009)

Detailed Description

Statement

Reference

Two pro-oocytes are visible in 69.6% of region 3 cysts in homozygous females (as assayed by c(3)G staining) compared to a frequency of 9.5% in wild type.

(Joyce and McKim, 2009)

The progeny of mutant females show a 90% decrease in meiotic crossover products and a 60% increase in noncrossover products (NCOs) compared to wild type. The frequency of postmeiotic segregation in NCOs of mutant females (16%) is significantly higher than that in NCOs from wild type (where postmeiotic segregation is exceedingly rare).

(Radford et al., 2007)

Mutant females show reduction in the overall frequency of crossing over on the second chromosome compared to wild type. The reduction in crossing over is the same in the centromere-proximal interval as in other regions of the chromosome.

(Blanton et al., 2005)

mei-9^a/mei-9^a mutant female meioses exhibit a lower frequency of recombination, and an increase in post-meiotic segregation, as compared to wild type.

(Bhagat et al., 2004)

mei-9^a/mei-9^A2 females show 30% X chromosome nondisjunction, compared to 0.33% in wild-type females. mei-9^a/mei-9^A2 larvae are hypersensitive to ultraviolet light and nitrogen mustard.

(Yildiz et al., 2004)

Mutants show an increased frequency of egg chamber degeneration after irradiation, and also "rescue" of some oocytes from induction of dominant lethal mutations after irradiation.

(Sarantseva and Khromykh, 2001)

The formation of mobile nucleoli is enhanced in these excision repair mutants.

(Semenov, 1998)

Excision repair capacity is deficient and post-replication repair capacity is normal.

(Ferro and Eeken, 1993)

Mutations exhibit an absolute deficiency in the capacity to excise UV-induced pyrimidine dimers (FBrf0028769).

(Todo and Ryo, 1992)

The median and maximal lifespans of mei-9^a flies are significantly lower than that of wild-type flies.

(Fernandez and Munoz, 1991)

Flies are hypersensitive to killing by γ-rays.

(Gamo et al., 1990)

Sensitive to methyl methanesulfonate.

(Venugopal et al., 1990)

Homozygotes have an increased frequency of X chromosome non-disjunction.

(Yamamoto and Wada, 1990)

Excision repair deficient.

(Eeken et al., 1985)

The presence or absence of mei-9^a has little effect on the reversion rate of RpII215⁹.

(Voelker et al., 1984)

Approximately 20% of embryos hatch.

(Dusenbery et al., 1983)

Reduces exchange uniformly throughout the euchromatin. Deleted chromatids are occasionally recovered.

(Carpenter and Baker, 1982)

Meiotic crossing-over is reduced by a factor of 1/12 in homozygous or mei-9^a/mei-9^b females. The frequency of gene conversion at the ry locus is unaffected in homozygous or mei-9^a/mei-9^b females. Post-meiotic segregation events, manifested as mosaic progeny, are produced at high frequency in homozygous and mei-9^a/mei-9^b females.

(Carpenter, 1982)

Homozygotes and hemizygotes show a higher frequency of spontaneous chromosome aberrations in neuroblast metaphases than wild-type larvae. The ratio of chromatid breaks to isochromatid breaks is 4.8-6.2. Approximately half of the breaks are heterochromatic and half are euchromatic. The breaks appear to be randomly distributed among the chromosomes. Breaks are 1.4-1.6 times more frequent in females than in males.

(Gatti, 1979)

mutagen sensitive reduces exchange mitotic instability

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

mei-9^a has abnormal meiotic cell cycle phenotype, suppressible by pch2^EY01788a

(Joyce and McKim, 2010)

NOT Suppressor of

Statement

Reference

mei-9^a is a non-suppressor of abnormal mitotic cell cycle phenotype of Fancm⁰⁶⁹³/Df(3R)ED6058

(Kuo et al., 2014)

slx1^F93I/mei-9^a is a non-suppressor of abnormal mitotic cell cycle phenotype of Fancm⁰⁶⁹³/Df(3R)ED6058

(Kuo et al., 2014)

Other

Statement

Reference

Fancm⁰⁶⁹³/Df(3R)ED6058, mei-9^a has viable phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, mei-9^a, slx1^F93I has viable phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mei-9^a has viable phenotype

(Kuo et al., 2014)

Df(3R)3450/yem¹, mei-9^a has abnormal meiotic cell cycle | female phenotype

(Meyer et al., 2010)

mei-218¹, mei-9^a has abnormal meiotic cell cycle phenotype

(Bhagat et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

mei-9^a has presumptive oocyte phenotype, suppressible by mei-218¹

(Joyce and McKim, 2009)

mei-9^a has phenotype, suppressible by rad201¹

(Sarantseva and Khromykh, 2001)

NOT Suppressor of

Statement

Reference

mei-9^a is a non-suppressor of phenotype of mei-41^D18

(McKim et al., 2000)

Other

Statement

Reference

grp^unspecified, mei-9^a has oocyte nucleus & meiotic cell cycle phenotype

(McKim et al., 2000)

mei-41^D18, mei-9^a has oocyte nucleus & meiotic cell cycle phenotype

(McKim et al., 2000)

mei-41[+]/mei-41^D18, mei-9^a has oocyte nucleus & meiotic cell cycle phenotype

(McKim et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The rate of spontaneous mitotic crossovers in Fancm⁰⁶⁹³/Df(3R)ED6058 males is not affected if animals are also mutant for mei-9^a, or if these animals are also mutant for both slx1^F93I and mei-9^a.

(Kuo et al., 2014)

mei-9^a ; yemα¹/Df(3R)3450 oocytes undergo precocious anaphase I (as occurs in mei-9^a single mutants), but meiosis II spindles are rarely observed in the double mutants (in contrast to mei-9^a single mutants where a greater proportion of the oocytes reach meiosis II).

(Meyer et al., 2010)

The high frequency of region 3 cysts containing two pro-oocytes that is seen in mei-9^a homozygous females is suppressed by mei-218¹.

(Joyce and McKim, 2009)

mei-9^a/mei-9^a mei-218¹/mei-218¹ double mutant female meioses exhibit a lower frequency of recombination, as compared to wild type, and a lower frequency of post-meiotic segregation, as compared to mei-9^a/mei-9^a single mutants.

(Bhagat et al., 2004)

About a quarter of nuclei in mei-9^a/mei-9^a; mei-41^D18/+ oocytes exhibit premature anaphase (or later) meioses. When mei-9^a is added to either grp^unspecified or grp^unspecified/+ flies, precocious anaphases are sometimes seen.

(McKim et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (15)

Bloomington

5671

y¹ mei-9^a mei-41^D5 wap⁹/FM7a

5672

y¹ mei-9^a rtv¹⁰ mei-41^D5/FM7c

5650

y¹ l(1)2Fd¹⁹ mei-9^a mei-41^D5/FM7c

5713

l(1)1Bm^8-12-2 mei-9^a/FM7c/Dp(1;Y)y²67g19.1

5669

y¹ mei-9^a mei-41^D5 l(1)19Ed¹⁷/FM7c

4185

y² sc¹ w^a mei-9^a/Dp(1;Y)y⁺

5401

y¹ mei-9^a mei-41^D5 l(1)ESHS46²/FM7c

Kyoto

108392

mei-9^a sw^20-14/FM7c, ct^S

107619

y² sc¹ w^a mei-9^a/Dp(1;Y)y⁺

108352

y¹ mei-9^a l(1)ESHS27¹ mei-41^D5/FM7c

108353

y¹ mei-9^a l(1)ESHS29¹ mei-41^D5/FM7c

101246

y² sc¹ w^a mei-9^a / Dp(1;Y)y⁺

More stocks available...

Notes on Origin

Discoverer

Comments

An endonuclease activity which is specific for partially depurinated DNA is reduced in mei-9^a cell lines and in extracts of mei-9^a larval brain ganglia compared to wild-type.

(Osgood and Boyd, 1982)

The activity of an apurinic (AP) endonuclease is reduced by 98% in mei-9^a mutants compared to wild-type. Mixing experiments between extracts of mei-9^a and wild-type embryos suggests that the mei-9 locus probably does not encode the AP endonuclease itself.

(Venugopal et al., 1990)

Simple meiotic gene conversion tracts produced in mei-9 mutants have been compared with those produced in wild-type.

(Curtis and Bender, 1991)

mei-9^a has no detectable effect on the recovery of chromosomes undergoing P-element transposition.

(Banga et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

9^a

(Gatti, 1979)

(Yildiz et al., 2004)

(Johnson-Schlitz et al., 2007)

mei-9

(Carpenter and Baker, 1982)

mei-9^a

(Hartmann et al., 2019, Kuo et al., 2014, Lafave et al., 2014, Joyce and McKim, 2010, Koana and Tsujimura, 2010, Meyer et al., 2010, Joyce and McKim, 2009, Radford et al., 2007, Blanton et al., 2005, Bhagat et al., 2004)

mei-9^α

(Varentsova and Khromykh, 1997)

mei-9a

(Sarantseva and Khromykh, 2001, Sarantseva and Khromykh, 2001, Kawai, 1997, Margulies, 1990)

mei9^a

(Brady et al., 2018, Sarantseva and Khromykh, 1999)

Name Synonyms

Secondary FlyBase IDs

References (58)

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