FlyBase Allele Report: Dmel\yem[1]

General Information

Symbol

Dmel\yem¹

Species

D. melanogaster

Name

FlyBase ID

FBal0248670

Feature type

allele

Associated gene

Dmel\yem

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Meyer et al., 2010)

Progenitor genotype

Cytology

Description

Amino acid replacement: V478E.

(Meyer et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:29,120,520..29,120,520 [-]

Nucleotide change:

T29120520A

Amino acid change:

V478E | yem-PA; V478E | yem-PB

Reported amino acid change:

V478E

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Meyer et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile (with yem²)

(Orsi et al., 2013)

female sterile | recessive

(Meyer et al., 2010)

lethal - all die during embryonic stage | maternal effect (with Df(3R)3450)

(Orsi et al., 2013)

lethal - all die during embryonic stage | maternal effect | recessive

(Meyer et al., 2010)

some die during embryonic stage | recessive | maternal effect | recessive

(Meyer et al., 2010)

Phenotype Manifest In

male pronucleus | maternal effect (with Df(3R)3450)

(Orsi et al., 2013)

oocyte (with Df(3R)3450)

(Meyer et al., 2010)

Detailed Description

Statement

Reference

Eggs from yemα¹/Df(3R)3450 females are normally fertilised and they initiate development, but the embryos die before hatching.

The male pronucleus fails to decondense at the pronuclear apposition stage in eggs derived from yemα¹/Df(3R)3450 females. Paternal chromosomes are subsequently excluded from the first zygotic division, resulting in gynogenetic haploid development and embryonic lethality.

(Orsi et al., 2013)

70% of eggs laid by homozygous females enter mitotic cycles bu the embryos fail to hatch.

Mature stage S14 yemα¹/Df(3R)3450 oocytes show chromosome defects. The symmetry of the congressed chromosome masses relative to the spindle and the symmetry of the congressed chromosome masses relative to the position of the achiasmate 4th chromosomes is affected in the mutant oocytes. The shape of the congressed exchange chromosome mass is also altered in the mutant oocytes, with a higher proportion of monolobed masses.

(Meyer et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Df(3R)3450/yem¹, mei-9^a has abnormal meiotic cell cycle | female phenotype

(Meyer et al., 2010)

Df(3R)3450/yem¹, mei-W68¹ has abnormal meiotic cell cycle | female phenotype

(Meyer et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

mei-W68¹ ; yemα¹/Df(3R)3450 oocytes undergo precocious anaphase I (as occurs in mei-W68¹ single mutants), but meiosis II spindles are rarely observed in the double mutants (in contrast to mei-W68¹ single mutants where a greater proportion of the oocytes reach meiosis II).

mei-9^a ; yemα¹/Df(3R)3450 oocytes undergo precocious anaphase I (as occurs in mei-9^a single mutants), but meiosis II spindles are rarely observed in the double mutants (in contrast to mei-9^a single mutants where a greater proportion of the oocytes reach meiosis II).

A small but significant proportion of eggs produced by mei-218¹/mei-218⁸ ; yemα¹/Df(3R)3450 females are able to develop into adults. These exceptional adults do not display paternal chromosome markers, suggesting that they have developed from diploid gametes that have undergone gynogenesis, a form of parthenogenesis that requires fertilisation.

(Meyer et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Df(3R)3450/yem¹ is rescued by yem^HPF.Tag:FLAG

(Orsi et al., 2013)

Comments

yemα^{HPF.T:Zzzz\FLAG} rescues the sterility of yemα¹/Df(3R)3450 females.

(Orsi et al., 2013)

Images (0)