FlyBase Allele Report: Dmel\mei-9[A2]

General Information

Symbol

Dmel\mei-9^A2

Species

D. melanogaster

Name

FlyBase ID

FBal0012174

Feature type

allele

Associated gene

Dmel\mei-9

Associated Insertion(s)

Carried in Construct

Also Known As

mei-9^AT2

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Yildiz et al., 2004)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C436T.

(Yildiz et al., 2004)

Amino acid replacement: R104term.

(Yildiz et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,317,487..4,317,487 [-]

Nucleotide change:

C4317487T

Reported nucleotide change:

C436T

Amino acid change:

R147term | mei-9-PA; R147term | mei-9-PB

Reported amino acid change:

R104term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement. Identical to mei-9^A1 and mei-9^A3.

(Yildiz et al., 2004, Smith, 1976)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Bloom syndrome

modeled by Blm^D2, Blm^N1

(Lafave et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Yildiz et al., 2002)

abnormal oxidative stress response

(Mishra et al., 2014)

chemical sensitive

(Radford et al., 2005, Yildiz et al., 2004, Yildiz et al., 2002)

chemical sensitive (with mei-9¹¹)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^a)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^b)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^D2)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^D4)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^L1)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^RT1)

(Yildiz et al., 2004)

chemical sensitive (with mei-9^RT3)

(Yildiz et al., 2004)

radiation sensitive

(Radford et al., 2005, Yildiz et al., 2004, Yildiz et al., 2002)

radiation sensitive (with mei-9¹¹)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^a)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^b)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^D2)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^D4)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^L1)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^RT1)

(Yildiz et al., 2004)

radiation sensitive (with mei-9^RT3)

(Yildiz et al., 2004)

Phenotype Manifest In

presumptive oocyte

(Joyce and McKim, 2009)

Detailed Description

Statement

Reference

Exposure of mei-9^A2 mutant larvae to DDVP for 48 hours results in a significant increase in the migration of DNA in midgut cells at 0.15, 1.5 and 15ng/ml concentrations of DDVP as compared to controls.

(Mishra et al., 2014)

Homozygous females show a high frequency (approximately 70%) of region 3 cysts with two pro-oocytes (as assayed by c(3)G staining) compared to a frequency of only 9.5% in wild type.

(Joyce and McKim, 2009)

Meiotic crossing over on chromosome 3 is reduced in mutant females compared to wild type.

(Trowbridge et al., 2007)

mei-9^A2 mutants have a similar frequency of single-strand annealing repair (SSA) compared to controls in a P{wIw.FRT} hemizygous assay to study DNA double-stranded break repair when assayed at 32^oC or 38^oC.

(Wei and Rong, 2007)

Mutant larvae are hypersensitive to UV light compared to wild-type. Mutants are also hypersensitive to methyl methanesulfonate. Mutants show a reduction in crossing over along the 3rd chromosome.

(Radford et al., 2005)

Homozygous females show 37% X chromosome nondisjunction, compared to 0.33% in wild-type females. mei-9^A2 shows increased X chromosome nondisjunction compared to the 0.33% seen in wild-type females, when in transheterozygous combination with mei-9^a (30%), mei-9^b (31%), mei-9^D2 (29%), mei-9^D4 (24%), mei-9^RT3 (22%), mei-9^L1 (37%), mei-9¹¹ (39%) or mei-9¹² (23%). mei-9^RT1/mei-9^A2 females show 2% X chromosome nondisjunction, compared to 0.33% in wild-type females. Homozygous females show a severe decrease in the level of crossing over along the left arm of chromosome 2 compared to wild type. mei-9^RT1/mei-9^A2 females show a relatively small decrease in the level of crossing over along the left arm of chromosome 2 compared to wild type. mei-9^RT1/mei-9¹² females show a severe decrease in the level of crossing over along the left arm of chromosome 2 compared to wild type. mei-9^RT1/mei-9^D4 females show a severe decrease in the level of crossing over along the left arm of chromosome 2 compared to wild type. mei-9^RT1/mei-9^RT3 females show a severe decrease in the level of crossing over along the left arm of chromosome 2 compared to wild type. Homozygous larvae are hypersensitive to ultraviolet light and nitrogen mustard. Larvae carrying mei-9^A2 in transheterozygous combination with one of mei-9^RT1, mei-9^b, mei-9¹¹, mei-9^D2, mei-9^L1, mei-9^a or mei-9^RT3 are hypersensitive to ultraviolet light and nitrogen mustard. mei-9¹²/mei-9^A2 larvae are not hypersensitive to ultraviolet light or nitrogen mustard. mei-9^D4/mei-9^A2 larvae are hypersensitive to ultraviolet light and show intermediate sensitivity to nitrogen mustard.

(Yildiz et al., 2004)

mei-9^A2 homozygotes a much lower meiotic crossover frequency than wild-type flies. Survival of mei-9^A2 homozygous adults exposed to UV, or treated with DNA damaging agents Nitrogen mustard or methyl methanesulfonate, is significantly reduced compared to wild-type. mei-9^A2 homozygotes have a X non-disjunction rate of 37%.

(Yildiz et al., 2002)

Show mitotic instability.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

mei-9^A2 is a non-suppressor of chemical sensitive phenotype of mus312^Z1973

(Yildiz et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

mei-9^A2 has presumptive oocyte phenotype, suppressible by pch2^EY01788a

(Joyce and McKim, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The high frequency of region 3 cysts containing two pro-oocytes that is seen in mei-9^A2 homozygous females is suppressed by pch2^EY01788a.

(Joyce and McKim, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mei-9^A2 is rescued by mei-9^+tSa

(Sekelsky et al., 1995)

Comments

mei-9^+tSa is able to rescue the MMS sensitivity of mei-9^A2 mutants and meiotic nondisjunction phenotype of mei-9^A2 females.

(Sekelsky et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

4280

w¹ mei-9^A2/C(1)DX, y¹ f¹

Notes on Origin

Discoverer

Alleles mei-9^A1, mei-9^A2 and mei-9^A3 contain the same molecular lesion.

(Yildiz et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

mei-9^A2

(Lafave et al., 2014, Mishra et al., 2014, Joyce and McKim, 2009, Trowbridge et al., 2007, Wei and Rong, 2007)

mei-9^AT2

(Bhui-Kaur et al., 1998, Sekelsky et al., 1995)

mei9^A2

(Cohen et al., 2003)

mus-110^AT2

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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