FlyBase Allele Report: Dmel\Mbs[03802]

(Cooper and Kennison, 2011, Lee and Treisman, 2004, Tan et al., 2003, Mizuno et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal recessive nonsyndromic deafness 2

modeled by ck⁰⁷¹³⁰, ck¹³

(Todi et al., 2008)

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Mbs⁰³⁸⁰² flies expressing Mbs^hs.PT at the start of embryogenesis (to rescue lethality) are fertile. Depletion of Mbs^hs.PT results in the formation of small ring canals at stage 10 but has no effect on the number of mitotic divisions or on the morphology of the fusome network. In contrast, the scra-stained nascent ring canals in the homozygous mutants are significantly smaller than the corresponding rings in the heterozygous flies at cyst stage IV-f, prior to ring canal growth.

Homozygous Mbs⁰³⁸⁰², transheterozygous Mbs⁰³⁸⁰²/Mbs^T541 and Mbs⁰³⁸⁰²/Mbs^T666 mutants exhibit small ring canals. The ring canals in homozygous mutants continue constricting after their final division.

Homozygous Mbs⁰³⁸⁰² germ-line clones exhibit a failure in nurse cell cytoplasm transport that leads to nurse cell dumping. Fast phase nurse cell cytoplasm transport is blocked in these mutants. Egg chambers with homozygous Mbs⁰³⁸⁰² somatic follicle cells develop normally. Centriole transport is disrupted in Mbs⁰³⁸⁰² homozygous germ-line clones. Most of the centrioles fail to migrate into the oocyte. Instead, they appear to accumulate at the nurse cell ring canals, on the nurse cell side, with no clusters detected within oocytes.

(Ong et al., 2010)

Homozygotes for Mbs⁰³⁸⁰² are lethal during early larval stages.

Cells within Mbs³ mutant clones in wing discs lose their columnar morphology and seem to retract basally.

(Mitonaka et al., 2007)

44% of Mbs⁰³⁸⁰² homozygotes die as embryos, and the remainder as 1st instar larvae. Over 80% of the dead mutant embryos have a dorsal hole in their cuticle, indicative of failure of dorsal closure. An even larger hole is seen in the cuticles of Mbs⁰³⁸⁰²/Df(3L)th102 embryos. Mbs⁰³⁸⁰² mutant embryos show occasional disruption of cell shape and elongation in leading edge cells towards the end of dorsal closure. Mbs⁰³⁸⁰² germ-line mutant eggs are rare and are approximately 1/4 the size of wild-type eggs. In Mbs⁰³⁸⁰² germ-line mutant egg chambers, dumping of nurse cell cytoplasm into the oocyte fails to occur properly. This is probably caused by defective growth of ring canals, which throughout oogenesis remain approximately the size of ring canals in wild-type stage 2 egg chambers.

(Tan et al., 2003)

Germline clones produce abnormal eggs due to abnormal oogenesis: few eggs are laid and they are tiny.

(Perrimon et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Mbs[+]/Mbs⁰³⁸⁰² is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

Mbs[+]/Mbs⁰³⁸⁰² is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Mbs[+]/Mbs⁰³⁸⁰² is an enhancer of nurse cell ring canal | germline clone phenotype of flw^XE55A

(Yamamoto et al., 2013)

Mbs[+]/Mbs⁰³⁸⁰² is an enhancer of interommatidial bristle phenotype of Rho1^GMR.PH

(Tan et al., 2003)

Mbs[+]/Mbs⁰³⁸⁰² is an enhancer of eye phenotype of Rho1^GMR.PH

(Tan et al., 2003)

Mbs[+]/Mbs⁰³⁸⁰² is an enhancer of eye phenotype of Rac1^GMR.PN

(Tan et al., 2003)

NOT Enhancer of

Statement

Reference

Mbs[+]/Mbs⁰³⁸⁰² is a non-enhancer of eye phenotype of Cdc42^GMR.PN

(Tan et al., 2003)

NOT Suppressor of

Statement

Reference

Mbs[+]/Mbs⁰³⁸⁰² is a non-suppressor of eye phenotype of Cdc42^GMR.PN

(Tan et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Mbs⁰³⁸⁰²/+ enhances the severity of the small ring canal phenotype seen in homozygous flw^XE55A germline clones. In addition, the eggs produced by the mutant females are further reduced in size compared to females carrying homozygous flw^XE55A germline clones in a wild-type background.

(Yamamoto et al., 2013)

The small, rough eye phenotype of Rac1^GMR.PN flies is enhanced by heterozygosity for Mbs⁰³⁸⁰². The rough eye phenotype of Rho1^GMR.PH flies is mildly enhanced by Mbs⁰³⁸⁰²/+ (fewer bristles are formed). The rough eye phenotype of Cdc42^GMR.PN flies in unaffected by heterozygosity for Mbs⁰³⁸⁰².

(Tan et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Mbs^T111

(Lee and Treisman, 2004)

Mbs^T541

(Lee and Treisman, 2004)

Mbs^T666

(Lee and Treisman, 2004)