FlyBase Allele Report: Dmel\ck[13]

General Information

Symbol

Dmel\ck¹³

Species

D. melanogaster

Name

FlyBase ID

FBal0001693

Feature type

allele

Associated gene

Dmel\ck

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Glowinski et al., 2014)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Gubb et al., 1984, O'Donnell et al., 1977)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Gubb et al., 1984)

Description

Amino acid replacement: R768term.

(Kiehart et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:15,049,991..15,049,991 [-]

Nucleotide change:

C15049991T

Amino acid change:

R768term | ck-PA; R768term | ck-PB; R768term | ck-PC; R768term | ck-PD

Reported amino acid change:

R768term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kiehart et al., 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of auditory system disease

CEA

(Todi et al., 2005)

model of autosomal recessive nonsyndromic deafness 2

CEC

(Todi et al., 2008)

CEC with ck⁰⁷¹³⁰

(Todi et al., 2008)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

FlyBase curator comment: "autosomal recessive nonsyndromic deafness 2" is associated with human MYO7A (the ortholog of ck).

(Todi et al., 2008)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

arista

(Kiehart et al., 2004)

brush border

(Glowinski et al., 2014)

brush border | somatic clone

(Glowinski et al., 2014)

follicle cell

(Glowinski et al., 2014)

follicle cell | somatic clone

(Glowinski et al., 2014)

Johnston's organ & dendritic cap cell

(Todi et al., 2005)

Johnston's organ & dendritic cap cell (with ck⁰⁷¹³⁰)

(Todi et al., 2005)

Johnston's organ & scolopidium

(Todi et al., 2005)

Johnston's organ & scolopidium (with ck⁰⁷¹³⁰)

(Todi et al., 2005)

macrochaeta

(Kiehart et al., 2004)

microchaeta

(Kiehart et al., 2004)

microvillus

(Glowinski et al., 2014)

microvillus | somatic clone

(Glowinski et al., 2014)

oocyte

(Glowinski et al., 2014)

trichogen cell

(Kiehart et al., 2004)

wing hair

(Gubb et al., 1984)

wing hair | ectopic

(Kiehart et al., 2004)

Detailed Description

Statement

Reference

Vitelline bodies have an irregular distribution appear shorter than normal in homozygous stage 10 follicle cells. The microvilli appear shorter than normal and appear to form a denser network than in wild-type follicle cells.

Vitelline bodies form narrower columns in mid-stage 10b follicles of mutant females. The follicle cell microvilli often show irregularities in orientation. The brush border of the oocyte is highly irregular and the surface of the vitelline bodies is jagged.

(Glowinski et al., 2014)

The morphology of follicle cell microvilli in homozygous females is indistinguishable from that of wild type.

(Demontis and Dahmann, 2009)

ck¹³ flies show no response to the pulse song, or to sine frequencies up to 1000 Hz, as measured via sound-evoked potentials from the antennal nerve. ck¹³/ck⁰⁷¹³⁰ transheterozygotes show a significantly reduced response to the pulse song compared to ck⁰⁷¹³⁰/+ controls. The Johnston's organ located at antennal segment 2 shows overall disorganization in ck¹³ mutants, with apical detachment of the scolopidia. The dendritic cap of the scolopidium often fails to enclose one of the cilia and may is sometimes divided into several compartments. However, scolopidial basal attachments to the a2 cuticle are not affected and a2/a3 joint epithelial cells are present, showing that detachment is not due to their degeneration. This phenotype is also observed in ck¹³/ck⁰⁷¹³⁰ transheterozygotes, although to a lesser extent. Expression of ck^{tail.Scer\UAS.T:Avic\GFP} in scolopale cells driven by Scer\GAL4^sqh.PW, or in neurons driven by Scer\GAL4^elav-C155, leads to a reduction in sound-evoked potentials from ck¹³/ck⁰⁷¹³⁰ flies.

(Todi et al., 2005)

ck¹³ flies are able to exclude markers from the cap cell-scolopale cell junction to the same extent as wild-type flies.

(Todi, 2005)

Nearly all homozygotes die as embryos. Mutant escapers are not fertile. Hemizygous escapers have a number of defects, including deep ridges in and aberrant projections from the shaft of the macrochaetae on the thorax. Both macrochaetae and microchaetae are stubby, branched, frequently twisted and not uniformly or smoothly tapered. Aristae are shorter and more highly branched than wild type. Wing hairs show a multiple wing hair phenotype.

(Kiehart et al., 2004)

Hemizygotes escape lethality with a frequency of 1%-2%. Escapers exhibit multiple trichomes on the wing. Escapers show two discrete periods of death, half as larvae and the remainder pupariate and develop into pharate adults, most die in the pupal case, only a few eclose.

(Gubb et al., 1984)

Class 2 (survival at >1% <5% expected).

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ck[+]/ck¹³ is a suppressor of visible phenotype of Scer\GAL4^en-e16E, zip^Rod.UAS.GFP

(Franke et al., 2010)

ck[+]/ck¹³ is a suppressor | partially of visible phenotype of Scer\GAL4^hs.PB, tow^UAS.cCa

(Chung et al., 2007)

ck¹³ is a suppressor of visible | recessive phenotype of dsh¹

(Winter et al., 2001)

ck[+]/ck¹³ is a suppressor of visible | heat sensitive phenotype of fz^I.hs

(Winter et al., 2001)

Phenotype Manifest In

Enhancer of

Statement

Reference

ck[+]/ck¹³ is an enhancer of eye phenotype of Rac1^GMR.PN

(Tan et al., 2003)

Suppressor of

Statement

Reference

ck[+]/ck¹³ is a suppressor of wing hair | increased number phenotype of Scer\GAL4^en-e16E, zip^Rod.UAS.GFP

(Franke et al., 2010)

ck[+]/ck¹³ is a suppressor | partially of wing hair | increased number phenotype of Scer\GAL4^hs.PB, tow^UAS.cCa

(Chung et al., 2007)

ck¹³ is a suppressor of wing hair | increased number phenotype of dsh¹

(Winter et al., 2001)

ck[+]/ck¹³ is a suppressor of wing hair | increased number phenotype of fz^I.hs

(Winter et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The multiple wing hair phenotype caused by expression of zip^{Rod.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^en-e16E is suppressed by ck¹³/+.

(Franke et al., 2010)

The multiple wing hair phenotype caused by expression of tow^Scer\UAS.cCa under the control of Scer\GAL4^hs.PB using heat shock at 24 hours after puparium formation is partially suppressed by one copy of ck¹³.

(Chung et al., 2007)

The polarity of hairs surrounding ck¹³, d^GC13 clones in the abdomen appear normal.

(Mao et al., 2006)

S^48-5/ck¹³ mutants show 5.41%+-2.22 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone.

(Gaengel and Mlodzik, 2003)

The small, rough eye phenotype of Rac1^GMR.PN flies is enhanced by heterozygosity for ck¹³.

(Tan et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ck¹³ is rescued by ck^UAS.GFP/Scer\GAL4^sqh.PW

(Todi et al., 2005)

Partially rescued by

ck¹³ is partially rescued by ck^UAS.GFP/Scer\GAL4^nompA.cK

(Todi et al., 2005)

ck¹³ is partially rescued by Scer\GAL4^elav-C155/ck^UAS.GFP

(Todi et al., 2005)

Comments

Expression of ck^{Scer\UAS.T:Avic\GFP} in the scolopale cells, driven by Scer\GAL4^nompA.cK, or in neurons, driven by Scer\GAL4^elav-C155, leads to a significant partial rescue of the ck¹³ auditory phenotype.

(Todi et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (10)

Bloomington

42722

w^* f^36a; P{f⁺13}30B ck¹³ P{neoFRT}40A/CyO

2202

f^36a; P{f⁺13}30B ck¹³ pr¹ pwn¹/CyO

6329

y¹; Dp(1;2)sc¹⁹, ck¹³ pr¹ pwn¹/CyO

5259

y¹; P{Car20y}25F ck¹³ P{neoFRT}40A/CyO; kar² ry⁵⁰⁶

43337

P{Ubx-FLP}1, y¹ w^*; P{Car20y}25F wb³ ck¹³ P{neoFRT}40A/CyO; P{neoFRT}82B P{πM}87E Sb^63b P{Car20y}96E/TM3, Ser¹

Kyoto

101563

wg^l-12 ck¹³ pr¹ cn¹ / CyO

108737

y¹; Dp(1;2)sc¹⁹, ck¹³ pr¹ pwn¹/CyO

108128

y¹; P{Car20y}25F ck¹³ P{neoFRT}40A/CyO; kar² ry⁵⁰⁶

102016

f^36a ; P{f⁺13}30B ck¹³ pr¹ pwn¹ / CyO ; mwh¹ jv¹

101288

Adh^D ck¹³ pr¹ cn¹ / CyO, Duox^Cy dpy^lv1 Adh^nB pr¹ cn²

Notes on Origin

Discoverer

Comments

Phenotypic data included in FBrf0051973.

(Roote, 1995.5.4)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CH-52

(O'Donnell et al., 1977)

CH52

ck-13

(Gaengel and Mlodzik, 2003)

ck¹³

(Sallee et al., 2021, Chen et al., 2018, Glowinski et al., 2014, Franke et al., 2010, Demontis and Dahmann, 2009, Todi et al., 2008, Christensen and Cook, 2007.3.22, Christensen and Cook, 2007.5.8, Chung et al., 2007, Mao et al., 2006)

ck^CH52

(Gubb et al., 1984)

ck^P13

(Tan et al., 2003)

l(2)br27^CH52

(Ashburner, 1982)

stch

(Baker, 2006.4.24)

Name Synonyms

Secondary FlyBase IDs

References (22)

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