FlyBase Allele Report: Dmel\Hph[02255]

General Information

Symbol

Dmel\Hph⁰²²⁵⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0009440

Feature type

allele

Associated gene

Dmel\Hph

Associated Insertion(s)

P{PZ}Hph⁰²²⁵⁵

Carried in Construct

Also Known As

l(3)02255

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

Progenitor genotype

Associated Insertion(s)

P{PZ}Hph⁰²²⁵⁵

Cytology

Description

P{PZ} insertion 336bp upstream of the initiation codon of Hph.

(Lavista-Llanos et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:5,265,203..5,265,203 [-]

(Doronkin et al., 2010, Centanin et al., 2005, Frei and Edgar, 2004, Lavista-Llanos et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size | adult stage (with Hph^S030304)

(Frei and Edgar, 2004)

decreased cell size | somatic clone | wandering third instar larval stage

(Frei and Edgar, 2004)

lethal (with Df(3R)3-4)

(Lavista-Llanos et al., 2002)

lethal | larval stage

(Frei and Edgar, 2004)

lethal | recessive

(Lavista-Llanos et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of first instar larval stage

(Centanin et al., 2005)

partially lethal - majority die (with Hph^S030304)

(Frei and Edgar, 2004)

partially lethal - majority die | embryonic stage

(Frei and Edgar, 2004)

partially lethal - majority die | embryonic stage (with Df(3R)3-4)

(Frei and Edgar, 2004)

partially lethal - majority die | larval stage (with Df(3R)3-4)

(Frei and Edgar, 2004)

some die during first instar larval stage

(Centanin et al., 2005)

some die during pupal stage (with Hph^S030304)

(Frei and Edgar, 2004)

Phenotype Manifest In

embryonic/larval fat body | somatic clone

(Frei and Edgar, 2004)

Detailed Description

Statement

Reference

(Frei and Edgar, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hph⁰²²⁵⁵/Hph^f03923, Scer\GAL4^slbo.2.6 is a suppressor | partially of abnormal cell migration phenotype of Scer\GAL4^slbo.2.6, sima^UAS.cBa

(Doronkin et al., 2010)

Hph[+]/Hph⁰²²⁵⁵ is a suppressor | partially of increased cell size | somatic clone phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Frei and Edgar, 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

Hph⁰²²⁵⁵/Hph^f03923, Scer\GAL4^slbo.2.6 is a suppressor | partially of border follicle cell phenotype of Scer\GAL4^slbo.2.6, sima^UAS.cBa

(Doronkin et al., 2010)

Hph[+]/Hph⁰²²⁵⁵ is a suppressor of wing disc | somatic clone | third instar larval stage phenotype of Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^Act5C.PP

(Datar et al., 2006)

Hph[+]/Hph⁰²²⁵⁵ is a suppressor | partially of ommatidium phenotype of Cdk4^UAS.cMa, CycD^UAS.cDa, Scer\GAL4^GMR.PF

(Frei and Edgar, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Trans-heterozygous Hph⁰²²⁵⁵/Hph^f03923 partially suppresses the block in migration caused by Scer\GAL4^slbo.2.6>sima^Scer\UAS.cBa expression.

(Doronkin et al., 2010)

Hph⁰²²⁵⁵/+ partially suppresses the increase in size of ommatidia and interommatidial bristles seen in seen in the eyes of Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF flies. FACS analysis of dissociated eye discs from Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF indicates that Scer\GAL4 expressing cells are larger than non-expressing and a decreased proportion are in S-phase. The increase in cell size, but not the reduction in S-phase, is partially suppressed by Hph⁰²²⁵⁵/+. FACS analysis of dissociated eye discs from Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF indicates that Scer\GAL4 expressing cells are larger than non-expressing. This increase in cell size is partially suppressed by Hph⁰²²⁵⁵/+.

(Frei and Edgar, 2004)

Xenogenetic Interactions

Statement

Reference

Clones of cells in the wing disc co-expressing Mmus\Ccnd1^Scer\UAS.cDa and Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP show a 60-70% increase in median clone size compared to wild-type controls. Hph⁰²²⁵⁵/+ suppresses this phenotype.

(Datar et al., 2006)

Complementation and Rescue Data

Fails to complement

Hph⁷⁸⁴

(Ghabrial et al., 2011)

Rescued by

Hph^S030304/Hph⁰²²⁵⁵ is rescued by Hph^UAS.cFa/Scer\GAL4^hs.PB

(Frei and Edgar, 2004)

Comments

P{UAS-Hph.F}II.1; P{UAS-Hph.F}II.2; Scer\GAL4^hs.PB rescues the lethality of Hph⁰²²⁵⁵/Hph^S030304 even in the absence of heat shock. Hph^Scer\UAS.cFa rescues the increase in ommatidial size in Cdk4^Scer\UAS.cMa; CycD^Scer\UAS.cDa; Scer\GAL4^GMR.PF suppressed by Hph⁰²²⁵⁵/+.

(Frei and Edgar, 2004)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11561

P{PZ}Hph⁰²²⁵⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Notes on Origin

Discoverer

A. Spradling.

Comments

P{PZ} inserted 104 bp upstream of putative transcription start site of Hph.

(Frei and Edgar, 2004)

Precise excision of the insertion reverts the mutant hypoxic response.

(Centanin et al., 2005)

Complements: l(3)01374⁰¹³⁷⁴. Complements: l(3)09904c^09904c. Complements: stumps^09904b. Complements: l(3)L3051^L3051.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Hph⁰²²⁵⁵

dHph⁰²²⁵⁵

(Ghabrial et al., 2011)

fatiga⁰²²⁵⁵

(Dong et al., 2015)

fga⁰²²⁵⁵

(Centanin et al., 2005)

hph⁰²²⁵⁵

(Doronkin et al., 2010, Datar et al., 2006)

l(3)02255

(Centanin et al., 2005, Frei and Edgar, 2004, Lavista-Llanos et al., 2002, Spradling et al., 1999)

l(3)02255⁰²²⁵⁵

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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