FlyBase Allele Report: Hsap\CDK4[UAS.cDa]

General Information

Symbol

Hsap\CDK4^UAS.cDa

Species

H. sapiens

Name

Saccharomyces cerevisiae UAS construct a of Datar

FlyBase ID

FBal0281053

Feature type

allele

Associated gene

Hsap\CDK4

Associated Insertion(s)

Carried in Construct

P{UAS-hCDK4.D}

Key Links

Transgenic product class

wild_type

(Datar et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Datar et al., 2006)

Mutagen

in vitro construct

(Datar et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-hCDK4.D}

Cytology

Description

UASt regulatory sequences drive expression of Hsap\CDK4.

(Datar et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Hsap\CDK4

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Hsap\CDK4^UAS.cDa/Scer\GAL4^da.G32 is a suppressor of abnormal size phenotype of Cdk4³

(Datar et al., 2006)

Other

Statement

Reference

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^GMR.PF has visible phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^GMR.PF has increased cell size phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^GMR.PF has abnormal size phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^Act5C.PP has abnormal endomitotic cell cycle | somatic clone | third instar larval stage phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^Act5C.PP has increased cell size | somatic clone | third instar larval stage phenotype

(Datar et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^Act5C.PP has wing disc | somatic clone | third instar larval stage phenotype, suppressible by Hph[+]/Hph⁰²²⁵⁵

(Datar et al., 2006)

Other

Statement

Reference

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^GMR.PF has eye phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^GMR.PF has ommatidium phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^Act5C.PP has wing disc | somatic clone | third instar larval stage phenotype

(Datar et al., 2006)

Hsap\CDK4^UAS.cDa, Mmus\Ccnd1^UAS.cDa, Scer\GAL4^Act5C.PP has embryonic/larval fat body | somatic clone | third instar larval stage phenotype

(Datar et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^da.G32 partially suppresses the reduced body weight of Cdk4³ flies.

Co-expression of Mmus\Ccnd1^Scer\UAS.cDa and Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF results in enlargement of the eye together with an increase in ommatidial size. Ommatidial packing is not disrupted and thus the eyes do not appear rough. Cells from third larval instar eye discs co-expressing Mmus\Ccnd1^Scer\UAS.cDa and Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^GMR.PF are 30% larger than wild-type controls, while cell proliferation is normal.

Clones of cells in the third larval instar fat body co-expressing Mmus\Ccnd1^Scer\UAS.cDa and Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP are larger than wild-type controls and contain more DNA.

Clones of cells in the wing disc co-expressing Mmus\Ccnd1^Scer\UAS.cDa and Hsap\CDK4^Scer\UAS.cDa under the control of Scer\GAL4^Act5C.PP show a 60-70% increase in median clone size compared to wild-type controls. Hph⁰²²⁵⁵/+ suppresses this phenotype.

(Datar et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\CDK4^Scer\UAS.cDa

Hsap\CDK4^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Datar

Secondary FlyBase IDs

References (1)

Report Sections

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