FlyBase Insertion Report: Dmel\P{PZ}Hph[02255]

General Information

Symbol

Dmel\P{PZ}Hph⁰²²⁵⁵

Species

D. melanogaster

Name

FlyBase ID

FBti0005451

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)02255

Affected gene(s)

Ecol\lacZ, Hph

Viability / fertility

Associated allele(s)

Ecol\lacZ^Hph-02255, Hph⁰²²⁵⁵

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 82F8 )

Sequence Location

3R:5,265,203..5,265,203 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:5,265,203..5,265,203 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

82F8 (inferred by FlyBase from sequence location)

Cytological location (reported)

82F4-82F7 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026177

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(French et al., 2003, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Hph

(Doronkin et al., 2010, Centanin et al., 2005, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Hph-02255

Phenotypic Class Phenotype Manifest in Detailed Description

Hph⁰²²⁵⁵

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

In wandering third instar larva, no detectable β-gal staining.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)02255

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11561

P{PZ}Hph⁰²²⁵⁵ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:1090924-1090925 determined by FlyBase alignment of dbGSS accession AQ026177 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1561

(Meister and Braun, 1995.10)

P{PZ}Hph⁰²²⁵⁵

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}l(3)02255

(Ostrowski et al., 2002)

P{PZ}l(3)02255⁰²²⁵⁵

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)02255⁰²²⁵⁵

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000512

References (16)

Report Sections

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