FlyBase Allele Report: Dmel\snRNP-U1-70K[02107]

General Information

Symbol

Dmel\snRNP-U1-70K⁰²¹⁰⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0007994

Feature type

allele

Associated gene

Dmel\snRNP-U1-70K

Associated Insertion(s)

P{PZ}snRNP-U1-70K⁰²¹⁰⁷

Carried in Construct

Also Known As

U1-70K¹, l(2)02107

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Tseng et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{PZ}snRNP-U1-70K⁰²¹⁰⁷

Cytology

Description

Insertion in the 5' UTR.

(Salz et al., 2004)

The P{PZ} insertion is 115bp upstream of the translation start site of snRNP70K.

(Cline et al., 1999)

P-element insertion in the 5' untranslated region.

(Tseng et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:6,971,656..6,971,656 [+]

(Sofola et al., 2007, Salz et al., 2004, Cline et al., 1999, Mount, 1999.5.23, Spradling et al., 1999, Tseng et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with snRNP-U1-70K²)

(Salz et al., 2004)

lethal | recessive

(Spradling et al., 1999, Tseng et al., 1998)

lethal - all die during embryonic stage | recessive

(Salz et al., 2004)

some die during embryonic stage | recessive

(Salz et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygotes complete embryogenesis with no consistent cuticular phenotype, but never hatch.

(Salz et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Sxl^fP7B0, snRNP-U1-70K⁰²¹⁰⁷, snf^J210 has lethal phenotype

(Nagengast et al., 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

snRNP-U1-70K⁰²¹⁰⁷ has phenotype, suppressible by snRNP-U1-70K::Hsap\SRSF1^Tag:FLAG

(Cline et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

When mothers heterozygous for both snf^J210 and snRNP70K⁰²¹⁰⁷ are crossed with Sxl^fP7B0 males, a significant reduction in viability is seen in Sxl^fP7B0/+ daughters. When mothers heterozygous for both snf^J210 and snRNP70K⁰²¹⁰⁷ are crossed with Sxl^fP7B0 males, a significant reduction in viability is seen in Sxl^fP7B0/+ daughters.

(Nagengast et al., 2003)

Xenogenetic Interactions

Statement

Reference

The addition of snRNP70K::Hsap\SFRS1^T:Zzzz\FLAG rescues the snRNP70K⁰²¹⁰⁷ phenotype.

(Cline et al., 1999)

Complementation and Rescue Data

Fails to complement

snRNP-U1-70K²

(Salz et al., 2004)

Rescued by

snRNP-U1-70K⁰²¹⁰⁷ is rescued by snRNP-U1-70K^hs.PS

(Salz et al., 2004)

snRNP-U1-70K⁰²¹⁰⁷ is rescued by snRNP-U1-70K^+t9

(Salz et al., 2004)

Comments

A transgene construct lacking the arginine-rich domain also rescues the lethality.

(Tseng et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11177

P{PZ}snRNP-U1-70K⁰²¹⁰⁷ cn¹/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

A. Spradling.

Comments

Excision of the P-element restores viability, as do transgenes carrying either a wildtype genomic fragment or cDNA under the control of the Hsp70 promoter. Some partial revertants show partial female-specific lethality and defects in oogenesis.

(Tseng et al., 1998)

"FBti0005210 == FBti0000289 == P{PZ}snRNP70K⁰²¹⁰⁷" was stated as revision.

(Mount, 1999.5.23)

Complements: Hrb27C⁰²⁶⁴⁷. Complements: smt3⁰⁴⁴⁹³. Complements: smt3⁰⁴⁸⁴¹. Complements: Hrb27C¹⁰²⁸⁰. Complements: smt3¹⁰⁶³⁶. Complements: milt^k06704. Complements: Coprox^k10617. Complements: milt^k14514b. Complements: cuc⁰⁶⁴⁹¹.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

70K¹

(Mount, 1999.5.23, Tseng et al., 1998)

U1-70K¹

(Salz et al., 2004, Nagengast et al., 2003, Cline et al., 1999)

U1-70k¹

(Penn et al., 2008)

l(2)02107

(Salz et al., 2004, Spradling et al., 1999)

l(2)02107⁰²¹⁰⁷

(Mount, 1999.5.23)

snRNP-U1-70K⁰²¹⁰⁷

snRNP27D⁰²¹⁰⁷

snRNP27D¹

snRNP70K⁰²¹⁰⁷

(Sofola et al., 2007)

snRNPU1-70K⁰²¹⁰⁷

(Carnesecchi et al., 2020)

Name Synonyms

Secondary FlyBase IDs

FBal0086333

References (12)

Report Sections

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