FlyBase Insertion Report: Dmel\P{PZ}snRNP-U1-70K[02107]

General Information

Symbol

Dmel\P{PZ}snRNP-U1-70K⁰²¹⁰⁷

Species

D. melanogaster

Name

FlyBase ID

FBti0005210

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02107

Affected gene(s)

Ecol\lacZ, snRNP-U1-70K

Viability / fertility

Associated allele(s)

Ecol\lacZ^{snRNP-U1-70K-02107}, snRNP-U1-70K⁰²¹⁰⁷

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 27D1 )

Sequence Location

2L:6,971,656..6,971,656 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:6,971,656..6,971,656 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

27D1 (inferred by FlyBase from sequence location)

Cytological location (reported)

27D1-27D2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025594

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

snRNP-U1-70K

(Sofola et al., 2007, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^{snRNP-U1-70K-02107}

Phenotypic Class Phenotype Manifest in Detailed Description

snRNP-U1-70K⁰²¹⁰⁷

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval brain | presumptive

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval peripheral nervous system

(Jackle and Janning, 1998.8.10)

embryonic/larval hindgut | anterior

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In embryo, expression in PNS not certain.

(Jackle and Janning, 1998.8.10)

In wandering third instar larva, weak expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02107

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11177

P{PZ}snRNP-U1-70K⁰²¹⁰⁷ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:6971656-6971657 confirmed by FlyBase alignment of dbGSS accession AQ025594 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1177

(Meister and Braun, 1995.10)

P{PZ}l(2)02107⁰²¹⁰⁷

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}snRNP-U1-70K⁰²¹⁰⁷

P{PZ}snRNP70K⁰²¹⁰⁷

(FlyBase, 2005, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02107⁰²¹⁰⁷

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000289

References (16)

Report Sections

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