FlyBase Allele Report: Dmel\cact[3]

General Information

Symbol

Dmel\cact³

Species

D. melanogaster

Name

FlyBase ID

FBal0001509

Feature type

allele

Associated gene

Dmel\cact

Associated Insertion(s)

Carried in Construct

Also Known As

cact^D13

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Bergmann et al., 1996)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Bergmann et al., 1996, Roth et al., 1991, Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Premature stop codon at amino acid 188.

(Bergmann et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,324,463..16,324,463 [-]

Nucleotide change:

C16324463T

Amino acid change:

Reported amino acid change:

?188term

Comment:

Reported as a premature stop codon at amino acid 188 (V). The point mutation is annotated as Q189@, since Gln to Stop is more likely than Val to Stop and the statement could be interpreted as a premature stop after codon 188.

(Bergmann et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Manfruelli et al., 1999, Qiu et al., 1998, Tearle and Nusslein-Volhard, 1987)

lethal - all die before end of larval stage (with cact^E8)

(Dolezal et al., 2005)

melanotic mass phenotype | third instar larval stage (with cact^E8)

(Dolezal et al., 2005)

some die during larval stage (with cact^E8)

(Dolezal et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

The mean circulating hemocyte number of cact^E10/cact³ mutants is not statistically different from that of controls.

(Chiu et al., 2005)

The ability of cact^E8/cact³ larvae to encapsulate L.boulardi eggs is significantly increased compared to that of control larvae.

(Sorrentino et al., 2004)

Lamellocytes appear in circulation during the second instar stage in cact⁷/cact³ larvae and are abundant in the hemolymph of third instar larvae (5-20%), where they participate in the formation of melanotic tumours. The crystal cell population is not affected. Lamellocytes are present in the lymph glands of third instar mutant larvae.

(Lanot et al., 2001)

cact^E8/cact³ larvae are viable at 24 and 48 hours after fertilisation. By 72 hours after fertilisation there is some lethality and delay in the rate of development compared to control siblings. Only 40% of the larvae survive to 120 hours after fertilisation, and only 4% of the larvae make white prepupae. Over 90% of larvae have melanotic capsules. Hemocyte density in cact^E8/cact³ hemolymph is more than 10-fold higher than in the hemolymph of control or heterozygous larvae. There are an increased number of lamellocytes in the hemolymph compared to control larvae. The lymph glands of 5 to 8 day old animals are abnormally large and there are many more hemocytes per lobe. Heterozygous adults do not have melanotic capsules.

(Qiu et al., 1998)

Embryos derived from germline clones show a strong ventralised phenotype but retain dorsoventral polarity.

(Bergmann et al., 1996)

Strong ventralizing phenotype: lack of all dorsally and laterally derived structures and the expansion of the ventral epidermis around the entire circumference. Expansion of twi expression in terminal regions.

(Roth et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cact³/cact^E8 has lethal | larval stage phenotype, suppressible | partially by Adgf-A^hs.PD

(Dolezal et al., 2005)

cact³ has lethal | recessive phenotype, suppressible by Df(2L)TW119

(Manfruelli et al., 1999)

NOT suppressed by

Statement

Reference

cact³/cact^E8 has melanotic mass phenotype | third instar larval stage phenotype, non-suppressible by Adgf-A^hs.PD

(Dolezal et al., 2005)

Other

Statement

Reference

Df(2L)TW119, cact³ has viable phenotype

(Manfruelli et al., 1999)

Tl³, cact³/cact[+] has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Tl^10b, cact³/cact[+] has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Tl[+]/Tl^10b, cact³ has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Tl[+]/Tl³, cact³ has melanotic mass phenotype phenotype

(Qiu et al., 1998)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

cact³/cact^E8 has melanotic mass | third instar larval stage phenotype, non-suppressible by Adgf-A^hs.PD

(Dolezal et al., 2005)

Other

Statement

Reference

Tl³, cact³/cact[+] has melanotic mass phenotype

(Qiu et al., 1998)

Tl^10b, cact³/cact[+] has melanotic mass phenotype

(Qiu et al., 1998)

Tl[+]/Tl^10b, cact³ has melanotic mass phenotype

(Qiu et al., 1998)

Tl[+]/Tl³, cact³ has melanotic mass phenotype

(Qiu et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The lethality associated with homozygous cact³ mutants is rescued by Df(2L)TW119, which removes Dif and dl.

(Manfruelli et al., 1999)

43% of cact³/+ ; Tl³/+ adults and 50.9% of cact³/+ ; Tl⁸/+ adults have melanotic capsules. No cact³/cact^BQ; Tl³/+ adults or cact³/cact^BQ; Tl⁸/+ adults have melanotic capsules.

(Qiu et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

cact³/cact⁷ is partially rescued by cact^UAS.cQa/Scer\GAL4^T98

(Qiu et al., 1998)

cact³/cact⁷ is partially rescued by Scer\GAL4^T59/cact^UAS.cQa

(Qiu et al., 1998)

cact³/cact⁷ is partially rescued by Scer\GAL4^e33C/cact^UAS.cQa

(Qiu et al., 1998)

cact³/cact⁵ is partially rescued by cact^UAS.cQa/Scer\GAL4^T98

(Qiu et al., 1998)

cact³/cact⁵ is partially rescued by Scer\GAL4^T59/cact^UAS.cQa

(Qiu et al., 1998)

cact³/cact⁵ is partially rescued by Scer\GAL4^e33C/cact^UAS.cQa

(Qiu et al., 1998)

cact³/cact⁵ is partially rescued by cact^UAS.cQa/Scer\GAL4^T100

(Qiu et al., 1998)

Not rescued by

cact³/cact⁷ is not rescued by cact^UAS.cQa/Scer\GAL4^e13C

(Qiu et al., 1998)

cact³/cact⁵ is not rescued by cact^UAS.cQa/Scer\GAL4^e13C

(Qiu et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

cact³

cact^D13

(Chiu et al., 2005, Dolezal et al., 2005, Sorrentino et al., 2004, Fernandez et al., 2001, Lanot et al., 2001, Rutschmann et al., 2000, Manfruelli et al., 1999, Qiu et al., 1998, Bergmann et al., 1996, Roth et al., 1991, Tearle and Nusslein-Volhard, 1987)

cactus^D13

(Novakova and Dolezal, 2011)

Name Synonyms

Secondary FlyBase IDs

References (13)

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