FlyBase Recombinant Construct Report: P{rk-GAL4.pan}

General Information

Symbol

P{rk-GAL4.pan}

FlyBase ID

FBtp0093267

Feature type

transgenic_transposable_element

Component Allele(s)

rk^pan.GAL4

Scer\GAL4^rk.pan

Also Known As

Rk^PAN-Gal4, RK-Gal4

Size

Expression Data

GAL4 reporter/driver

Associated insertion(s)

0 available

Molecular map

Description and Uses

Construct components

Component allele Scer\GAL4^rk.pan

Transgenic product class

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Description

The "T2A" peptide from Thosea asigna followed by the GAL4 coding region has been inserted within exon 14 of rk within the CH322-119A8 Pacman genomic clone that contains the complete rk locus. Exon 14 is shared by all predicted rk splice variants. The insertion of this sequence results in a non-functional truncated rk protein being produced. The "T2A" peptide supports ribosomal skipping, allowing the GAL4 protein to be translated independently of the upstream rk protein sequences, resulting in functional GAL4 protein being expressed under the control of rk regulatory sequences.

(Diao and White, 2012)

Component allele rk^pan.GAL4

Transgenic product class

inframe_deletion

(Diao and White, 2012)

loss_of_function_variant

(Diao and White, 2012)

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Description

The "T2A" peptide from Thosea asigna followed by the Scer\GAL4 coding region has been inserted within exon 14 of rk within the CH322-119A8 Pacman genomic clone that contains the complete rk locus. Exon 14 is shared by all predicted rk splice variants. The insertion of this sequence results in a non-functional truncated rk protein being produced. The "T2A" peptide supports ribosomal skipping, allowing the Scer\GAL4 protein to be translated independently of the upstream rk protein sequences, resulting in functional Scer\GAL4 protein being expressed under the control of rk regulatory sequences.

(Diao and White, 2012)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

rk^pan.GAL4

Phenotypic Class Phenotype Manifest In Detailed Description

Scer\GAL4^rk.pan

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

P-stage

neuron | subset of pupal central nervous system

(Diao et al., 2017)

pupal stage -- adult stage

adult midgut muscle cell

(Scopelliti et al., 2014)

pharate adult stage

adult central nervous system

(Diao and White, 2012)

adult stage | female

spermatheca

(Diao and White, 2012)

adult stage

adult midgut

(Diao and White, 2012)

adult visceral muscle cell of adult midgut region R4

(Scopelliti et al., 2019)

adult visceral muscle cell of adult midgut region R5

(Scopelliti et al., 2019)

terminal tracheal cell | subset

(Scopelliti et al., 2019)

neuron | subset of adult central nervous system

(Scopelliti et al., 2019)

nervus corporis cardiaci

(Scopelliti et al., 2019)

adult stage A1

wing

(Diao and White, 2012)

adult epidermis

(Diao and White, 2012)

Additional Information

Statement

Reference

ScerGAL4^rk.pan drives expression in the visceral muscle of the adult midgut in the region R4/R5 transition region. ScerGAL4^rk.pan also drives expression in terminal tracheal cells in close proximity to adult fat bodies, and in neurons of the corpus cardiacum.

(Scopelliti et al., 2019)

ScerGAL4^rk.pan drives expression in a subset of CNS neurons synaptically downstream of CCAP/burs neurons. The expression pattern is almost entirely non-overlapping with that of ScerGAL4^{CCAP-R-MI05804-TG4.1}, which drives in the pattern of the CCAP receptor.

(Diao et al., 2017)

ScerGAL4^rk.pan drives expression in the visceral muscle of the midgut at pupal and adult stages.

(Scopelliti et al., 2014)

ScerGAL4^rk.pan drives expression in a pattern fully recapitulating that of endogenous rk, including in the epidermis and unexpanded wings of newly eclosed flies, in the pharate adult CNS, in both virgin and mated spermatheca, and in the adult midgut.

(Diao and White, 2012)

Marker for

Reflects expression of

(Scopelliti et al., 2019, Scopelliti et al., 2014, Diao and White, 2012)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

descendant(s)

Comments

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

P{rk-GAL4.pan}

(FlyBase, 1992-)

RK-Gal4

(Diao et al., 2017)

Rk-Gal4

(Diao et al., 2017)

Rk^PAN-Gal4

(Harwood et al., 2014)

Rk^pan-Gal4

(Diao et al., 2017)

lgr2^PAN-Gal4

(Scopelliti et al., 2019)

rk GAL4

(Melnattur et al., 2020)

rk-gal4

(Melnattur et al., 2020)

rk-pan GAL4

(Melnattur et al., 2020)

rk^pan-Gal4

(Luan et al., 2020)

rk^pangal4

(Scopelliti et al., 2014)

Secondary FlyBase IDs

References (8)

Report Sections

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