FlyBase Allele Report: Scer\GAL4[rk.pan]

General Information

Symbol

Scer\GAL4^rk.pan

Species

S. cerevisiae

Name

FlyBase ID

FBal0295408

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{rk-GAL4.pan}

Also Known As

Rk^PAN-Gal4, RK-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Diao and White, 2012)

Progenitor genotype

Carried in construct

P{rk-GAL4.pan}

Cytology

Description

The "T2A" peptide from Thosea asigna followed by the GAL4 coding region has been inserted within exon 14 of rk within the CH322-119A8 Pacman genomic clone that contains the complete rk locus. Exon 14 is shared by all predicted rk splice variants. The insertion of this sequence results in a non-functional truncated rk protein being produced. The "T2A" peptide supports ribosomal skipping, allowing the GAL4 protein to be translated independently of the upstream rk protein sequences, resulting in functional GAL4 protein being expressed under the control of rk regulatory sequences.

(Diao and White, 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

P-stage

neuron | subset of pupal central nervous system

(Diao et al., 2017)

pupal stage -- adult stage

adult midgut muscle cell

(Scopelliti et al., 2014)

pharate adult stage

adult central nervous system

(Diao and White, 2012)

adult stage | female

spermatheca

(Diao and White, 2012)

adult stage

adult midgut

(Diao and White, 2012)

adult visceral muscle cell of adult midgut region R4

(Scopelliti et al., 2019)

adult visceral muscle cell of adult midgut region R5

(Scopelliti et al., 2019)

terminal tracheal cell | subset

(Scopelliti et al., 2019)

neuron | subset of adult central nervous system

(Scopelliti et al., 2019)

nervus corporis cardiaci

(Scopelliti et al., 2019)

adult stage A1

wing

(Diao and White, 2012)

adult epidermis

(Diao and White, 2012)

Additional Information

Statement

Reference

ScerGAL4^rk.pan drives expression in the visceral muscle of the adult midgut in the region R4/R5 transition region. ScerGAL4^rk.pan also drives expression in terminal tracheal cells in close proximity to adult fat bodies, and in neurons of the corpus cardiacum.

(Scopelliti et al., 2019)

ScerGAL4^rk.pan drives expression in a subset of CNS neurons synaptically downstream of CCAP/burs neurons. The expression pattern is almost entirely non-overlapping with that of ScerGAL4^{CCAP-R-MI05804-TG4.1}, which drives in the pattern of the CCAP receptor.

(Diao et al., 2017)

ScerGAL4^rk.pan drives expression in the visceral muscle of the midgut at pupal and adult stages.

(Scopelliti et al., 2014)

ScerGAL4^rk.pan drives expression in a pattern fully recapitulating that of endogenous rk, including in the epidermis and unexpanded wings of newly eclosed flies, in the pharate adult CNS, in both virgin and mated spermatheca, and in the adult midgut.

(Diao and White, 2012)

Marker for

Reflects expression of

(Scopelliti et al., 2019, Scopelliti et al., 2014, Diao and White, 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )