FlyBase Allele Report: Dmel\Burs::Pburs[UAS.Tag:TM(hTNF),mCherry]

General Information

Symbol

Dmel\Burs::Pburs^{UAS.Tag:TM(hTNF),mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0318510

Feature type

allele

Associated gene

Burs::Pburs

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry-tBur-β-α}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Harwood et al., 2014)

Progenitor genotype

Carried in construct

P{UAS-mCherry-tBur-β-α}

Cytology

Description

UASt regulatory sequences drive expression of a membrane-anchored fusion protein encoding the two subunits of the bursicon heterodimer (Pburs followed by Burs) in a single protein. The fusion protein is tagged at the N-terminal end with mCherry, and then consists of a membrane tethering signal (Tag:TM(hTNF)), the Pburs coding region and the Burs coding region.

(Harwood et al., 2014)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Burs::Pburs

Tagged with

mCherry

red fluorescent protein

Tag:TM(hTNF)

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^Act5C.PI

(Harwood et al., 2014)

partially lethal - majority die, with Scer\GAL4^Act88F.1.3

(Harwood et al., 2014)

partially lethal - majority die, with Scer\GAL4^ap-md544

(Harwood et al., 2014)

partially lethal - majority die, with Scer\GAL4^how-24B

(Harwood et al., 2014)

partially lethal - majority die, with Scer\GAL4^rk.pan

(Harwood et al., 2014)

partially lethal - majority live, with Scer\GAL4^Mef2.PR

(Harwood et al., 2014)

partially lethal - majority live, with Scer\GAL4^Toll-6-D42

(Harwood et al., 2014)

visible, with Scer\GAL4^Act5C.PI

(Harwood et al., 2014)

visible, with Scer\GAL4^Act88F.1.3

(Harwood et al., 2014)

visible, with Scer\GAL4^ap-md544

(Harwood et al., 2014)

visible, with Scer\GAL4^CCAP.PU

(Harwood et al., 2014)

visible, with Scer\GAL4^how-24B

(Harwood et al., 2014)

visible, with Scer\GAL4^Mef2.PR

(Harwood et al., 2014)

visible, with Scer\GAL4^rk.pan

(Harwood et al., 2014)

visible, with Scer\GAL4^Toll-6-D42

(Harwood et al., 2014)

Phenotype Manifest In

wing, with Scer\GAL4^Act5C.PI

(Harwood et al., 2014)

wing, with Scer\GAL4^Act88F.1.3

(Harwood et al., 2014)

wing, with Scer\GAL4^ap-md544

(Harwood et al., 2014)

wing, with Scer\GAL4^CCAP.PU

(Harwood et al., 2014)

wing, with Scer\GAL4^how-24B

(Harwood et al., 2014)

wing, with Scer\GAL4^Mef2.PR

(Harwood et al., 2014)

wing, with Scer\GAL4^rk.pan

(Harwood et al., 2014)

wing, with Scer\GAL4^Toll-6-D42

(Harwood et al., 2014)

Detailed Description

Statement

Reference

Expression of Burs::Pburs^{Scer\UAS.T:Hsap\TM-TNF,T:Disc\RFP-mCherry} driven by Scer\GAL4^Act5C.PI or Scer\GAL4^rk.pan results in a significant decrease in survival through eclosion (less than 20% or 40% survive, respectively); escapers fail to expand their wings (100%). When expression is driven by Scer\GAL4^CCAP.PI, flies do not have reduced survival but have wing expansion defects; when driven by Scer\GAL4^how-24B, Scer\GAL4^Toll-6-D42, Scer\GAL4^ap-md544, Scer\GAL4^Mef2.PR or Scer\GAL4^Act88F.1.3 flies have both significantly reduced survival (survival of around 20%, 80%, 10%, 80%, 10%) and escapers have wing expansion defects (100%, 30%, 100%, 35%, 100%).

(Harwood et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Burs::Pburs^{Scer\UAS.T:Hsap\TM-TNF,T:Disc\RFP-mCherry}

Burs::Pburs^{UAS.Tag:TM(hTNF),mCherry}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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