The "T2A" peptide from Thosea asigna followed by the Scer\GAL4 coding region has been inserted within exon 14 of rk within the CH322-119A8 Pacman genomic clone that contains the complete rk locus. Exon 14 is shared by all predicted rk splice variants. The insertion of this sequence results in a non-functional truncated rk protein being produced. The "T2A" peptide supports ribosomal skipping, allowing the Scer\GAL4 protein to be translated independently of the upstream rk protein sequences, resulting in functional Scer\GAL4 protein being expressed under the control of rk regulatory sequences.
rkpan.GAL4 fails to rescue rk4
rkpan.GAL4 fails to rescue the wing expansion defects of rk4 mutant flies.