FB2024_03 , released June 25, 2024
Allele: Dmel\rkpan.GAL4
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General Information
Symbol
Dmel\rkpan.GAL4
Species
D. melanogaster
Name
FlyBase ID
FBal0295410
Feature type
allele
Associated gene
Dmel\rk
Associated Insertion(s)
Carried in Construct
P{rk-GAL4.pan}
Also Known As
RkPAN-Gal4, RK-Gal4
Key Links
Transgenic product class
inframe_deletion
(Diao and White, 2012)
loss_of_function_variant
(Diao and White, 2012)
Mutagen
Nature of the Allele
Transgenic product class
inframe_deletion
(Diao and White, 2012)
loss_of_function_variant
(Diao and White, 2012)
Mutagen
in vitro construct
(Diao and White, 2012)
Progenitor genotype
Carried in construct
P{rk-GAL4.pan}
Cytology
Description

The "T2A" peptide from Thosea asigna followed by the Scer\GAL4 coding region has been inserted within exon 14 of rk within the CH322-119A8 Pacman genomic clone that contains the complete rk locus. Exon 14 is shared by all predicted rk splice variants. The insertion of this sequence results in a non-functional truncated rk protein being produced. The "T2A" peptide supports ribosomal skipping, allowing the Scer\GAL4 protein to be translated independently of the upstream rk protein sequences, resulting in functional Scer\GAL4 protein being expressed under the control of rk regulatory sequences.

(Diao and White, 2012)
Allele components
Component
Use(s)
Regulatory region(s)
rk
Encoded product / tool
rk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      rkpan.GAL4 fails to rescue rk4

      (Diao and White, 2012)
      Comments

      rkpan.GAL4 fails to rescue the wing expansion defects of rk4 mutant flies.

      (Diao and White, 2012)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      rkpan.GAL4
      Name Synonyms
      Secondary FlyBase IDs
        References (2)