FlyBase Experimental Tool Report: Tag:M(rCd2)

FB2024_03 , released June 25, 2024

FB2024_03 , released June 25, 2024

Experimental tool: Tag:M(rCd2)

General Information

Symbol

Tag:M(rCd2)

FlyBase ID

FBto0000214

Name

Membrane tethering sequence from rCd2

Description

Description

Sequence from the Rattus norvegicus Cd2 gene (RGD:2297) that results in a tagged protein being tethered to the membrane (may comprise the entire Cd2 gene or only a part of it).

Uses

membrane protein localization tag

External Crossreferences and Linkouts

Related experimental tools

Transgenic Constructs

Tagged with the tool (24)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

PBac{UAS(B3RT.B2)BoNTA}

Cbot\BoNT-A^{B3RT.B2.UAS.VHH(GFP4),Tag:M(rCd2),Tag:CS(TEVp)}

UAS

B2R: recombinase

Tag:M(rCd2): membrane protein localization tag
Tag:CS(TEVp): protease target site
VHH(GFP4): nanobody

B3RT: recombinase target site

PBac{UAS-extra.SNAPf.CD2}

Zzzz\SNAP^{SNAPf.extra.UAS.CD2}

UASt

SNAPf-tag: chemical labeling reporter

Tag:M(rCd2): membrane protein localization tag
Tag:SS(pat-3): signal sequence

P{LexAop2(RSRT.Flp1)TEV.CD2.P10}

TEV\NIa^{RSRT.Flp1.LexAop2.Tag:M(rCd2),Tag:MYC}

lexAop

FLPD5: recombinase

Tag:MYC: epitope tag
Tag:M(rCd2): membrane protein localization tag

RSRT: recombinase target site

P{LexAop2(RSRT.Flp1)TEV^3xmut.CD2.P10}

TEV\NIa^{3xmut.RSRT.Flp1.LexAop2.Tag:M(rCd2),Tag:MYC}

lexAop

FLPD5: recombinase
TEVp(3xmut): protease

Tag:MYC: epitope tag
Tag:M(rCd2): membrane protein localization tag

RSRT: recombinase target site

P{LexAop2(RSRT.Flp1)TEV^T173V.CD2.P10}

TEV\NIa^{T173V.RSRT.Flp1.LexAop2.Tag:M(rCd2),Tag:MYC}

lexAop

FLPD5: recombinase
TEVp(T173V): protease

Tag:MYC: epitope tag
Tag:M(rCd2): membrane protein localization tag

RSRT: recombinase target site

TEV\NIa^{LexAop2.Tag:M(rCd2),Tag:MYC}

lexAop

TEV\NIa

Tag:MYC: epitope tag
Tag:M(rCd2): membrane protein localization tag

RSRT: recombinase target site

P{LexAop2-TEV^3xmut.CD2.P10}

TEV\NIa^{3xmut.LexAop2.Tag:M(rCd2),Tag:MYC}

lexAop

TEVp(3xmut): protease

Tag:MYC: epitope tag
Tag:M(rCd2): membrane protein localization tag

RSRT: recombinase target site

P{LexAop2-TEV^T173V.CD2.P10}

TEV\NIa^{T173V.LexAop2.Tag:M(rCd2),Tag:MYC}

lexAop

TEVp(T173V): protease

Tag:MYC: epitope tag
Tag:M(rCd2): membrane protein localization tag

RSRT: recombinase target site

P{sog.eve.st2.T:CD2}

sog^CD2.st2.eve

eve

sog

Tag:M(rCd2): membrane protein localization tag

FRT: recombinase target site

P{sog.FRT.st2.T:CD2}

sog^{CD2.FRT.st2.eve}

eve

sog

Tag:M(rCd2): membrane protein localization tag

FRT: recombinase target site

P{UAS-crb.Intra16-29-WT.T:CD2}

crb^{CD2.Intra16-29.UAS}

UASt

crb

Tag:M(rCd2): membrane protein localization tag

P{UAS-crb.Intra16-37-WT.T:CD2}

crb^{CD2.Intra16-37.UAS}

UASt

crb

Tag:M(rCd2): membrane protein localization tag

P{UAS-crb.IntraCE.T:CD2}

Cele\crb-1^{CD2.IntraCE.UAS}

UASt

Cele\crb-1

Tag:M(rCd2): membrane protein localization tag

P{UAS-crb.IntraWT.T:CD2}

crb^{CD2.IntraWT.UAS}

UASt

crb

Tag:M(rCd2): membrane protein localization tag

P{UAS-dlp.-HS.CD2}

dlp^-HS.CD2.UAS

UASt

dlp

Tag:M(rCd2): membrane protein localization tag

P{UAS-dlp.-HS.GFP.CD2}

dlp^{-HS.CD2.UAS.EGFP}

UASt

dlp

EGFP: green fluorescent protein
Tag:M(rCd2): membrane protein localization tag

P{UAS-dlp.N-CD2}

UASt

dlp

Tag:M(rCd2): membrane protein localization tag

P{UAS-GFP-Bsg.extra.CD2}

Bsg^{extra.UAS.Tag:M(rCd2),GFP}

UAS

Bsg

GFP: green fluorescent protein
Tag:M(rCd2): membrane protein localization tag

P{UAS-GFP-dlp-CD2}

dlp^{UAS.GFP,Tag:M(rCd2)}

UAS

dlp

GFP: green fluorescent protein
Tag:M(rCd2): membrane protein localization tag

P{UAS-hhCD2}

UASt

hh

Tag:M(rCd2): membrane protein localization tag

P{UAS-spi.CS.s.CD2.HA}

spi^{CS.s.UAS.Tag:M(rCd2).Tag:HA}

UAS

spi

Tag:HA: epitope tag
Tag:M(rCd2): membrane protein localization tag

PBac{UAS(B3RT.B2)HBMBoNTA}

Cbot\BoNT-A^{B3RT.B2.HBM.UAS.VHH(GFP4),Tag:M(rCd2),Tag:CS(TEVp)}

UAS

B2R: recombinase

Tag:M(rCd2): membrane protein localization tag
Tag:CS(TEVp): protease target site
VHH(GFP4): nanobody

B3RT: recombinase target site

PBac{UAS-BoNTA}

Cbot\BoNT-A^{UAS.VHH(GFP4),Tag:M(rCd2),Tag:CS(TEVp)}

UAS

Cbot\BoNT-A

Tag:M(rCd2): membrane protein localization tag
Tag:CS(TEVp): protease target site
VHH(GFP4): nanobody

B3RT: recombinase target site

PBac{UAS-orion.B.CD2.mIFP}

orion^{UAS.B.CD2,mIFP}

UAS

orion

mIFP: infra-red fluorescent protein
Tag:M(rCd2): membrane protein localization tag

Insertions ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tag:M(rCd2)

Tag:TM(rCd2)

Name Synonyms

Membrane tethering sequence from rCd2

Secondary FlyBase IDs

References (11)

Report Sections